Dataset: Phillips_2011 - Experiment 1 Larval Size

In this experiment, larvae were placed into feeding treatments with different types of phytoplankton to determine larval nutritional strategies.

Larvae hatched on Sept 24, 2009 and were distributed into tubs on 500mL filtered sea water (FSW). Three larval feeding treatments with different species of phytoplankton, all at 10 x 104 cells mL-1: Isochrysis galbana (‘‘Iso’’ treatment), Dunaliella tertiolecta (‘‘Dun’’ treatment), a 1:1 ratio of I. galbana and D. tertio- lecta (‘‘Mixed’’ treatment), plus an Unfed treatment in which larvae were raised in FSW. Investigators used a hemocytometer to count algal cells and calculate densities of phytoplankton stocks and amount of stock to add to containers for each treatment. On days 3, 6, 9 and 18, six larvae per container were haphazardly sampled and preserved them in 70% ethanol for later measurement of protoconch height Protoconch height was measured in microns. Each larvae was sampled once. Sometimes only 5 larvae were in the preserved vials, so the 6th one is marked as missing. On day 0 (day of hatch) larvae were samples from those placed in experiment, but the data are not included in any analysis.