Dataset: Rapid Light Curves_PAM

Name: Measurements of fluorescence of photosystem II in Plocamium cartilagineum under various and pCO2 and temperature conditions
Published: 2018-04-11
Keywords: biota, oceans

Cite This Dataset

DOI:10.1575/1912/bco-dmo.733238.1

Spatial Extent: N:34 E:-118 S:33 W:-119

Temporal Extent: 2014-06-26 - 2015-02-12

Project:

Ocean Acidification: Scope for Resilience to Ocean Acidification in Macroalgae (Seaweed OA Resilience)

Program:

Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA) (SEES-OA)

Principal Investigator:

Janet E. Kubler (California State University Northridge, CSUN)

Co-Principal Investigator:

Steve Dudgeon (California State University Northridge, CSUN)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2018-04-11

Restricted:

Release Date:

2019-06-03

Validated:

Yes

Current State:

Final no updates expected

Measurements of fluorescence of photosystem II in Plocamium cartilagineum under various and pCO2 and temperature conditions

Abstract:

This dataset includes photosynthetic pigment concentrations in Plocamium cartilagineum grown under various temperatures and CO2 levels, from July 2014 to February 2015. The parameters reported are: the concentrations of Chlorophyll a, Phycoerythrin, Phycocyanin, Allophycocyanin, and total Phycobiliprotein, and the ratio of total Phycobiliprotein to Chlorophyll A.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2018-04-11	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.1575/1912/bco-dmo.733238.1	2019-06-03

Description

Related Datasets:
Plocamium carbon nitrogen and stable isotopes: Plocamium carbon and nitrogen content and stable isotope values, 2014-2015 (Seaweed OA Resilience project)

Plocamium culture carbonate chemistry: Carbonate chemistry in experimental cultures of Plocamium cartilagineum cultured at different temperatures and pCO2 levels (Seaweed OA Resilience project)

Plocamium culture: seawater delta13C: Stable isotope ratio and concentration of carbon in seawater during Plocamium culture experiments, 2014-2015 (Seaweed OA Resilience project)

Plocamium cultures pH and temperature: Plocamium culture pot pH and temperature time-series at 10 minute sampling intervals from 2014-2015 (Seaweed OA Resilience project)

Plocamium exptl treatments summary: Summary of pCO2 and temperature treatment combinations for each culture pot and experimental trial (Seaweed OA Resilience project)

Plocamium growth and biomass: Experimental results of Plocamium cartilagineum growth and biomass as a function of pCO2 and temperature (Seaweed OA Resilience project)

Plocamium: pH drift: Carbonate chemistry over a time-course in pH drift experiments with Plocamium growth collected at Catalina Island, 2014-2015 (Seaweed OA Resilience project)

Methods & Sampling

Plocamium cartilagineum was collected from Catalina Island in June - Nov. 2014 and Jan. 2015.

Culture pots were placed in large thermally insulated coolers in a temperature-controlled water bath at either 15 or 20°C under saturating illumination of ~150 µmoles photons/m^2/s. pCO2 treatments were supplied to closed culture pots by use of a gas mixing system combining Nitrogen, Oxygen and Carbon Dioxide to specific CO2 partial pressures, 20.9% oxygen and the balance being Nitrogen.

Measurements of chlorophyll fluorescence of photosystem II were made using a pulse amplitude modulated (PAM) fluorometer in situ in culture pots. All samples were dark-adapted prior to measurement by placing a leaf clip in the closed position over the area to be sampled for 5 minutes prior to measurement. For fluorescence measurements, the fiber optic probe was placed directly on the leaf clip perpendicular to the sample. The leaf clip was then opened and a rapid light curve program function was initiated to measure fluorescence at each of 9 photon flux densities (PFD’s). PFD’s of the light curve generated by the fluorometer were calibrated using an external LiCor light meter. Following measurement, the leaf clip was removed and the sample remained in the culture chamber.

Data were downloaded from the Diving PAM using WinControl version 3 software for Diving PAM.

Note: Trial 1 was a pilot test of culture system and methodological procedures so was not used for data collection in the testing of hypotheses.

See Supplemental Files for a table of culture conditions for each of the 8 trials (pdf).

Data Processing Description

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- re-formatted date from dd.mm.yy to yyyy-mm-dd
- reordered columns to correspond with good database practices

Data Files

File
RLC_PAM.csv (Comma Separated Values (.csv), 500.10 KB) MD5:28511621764b1616f2e73fced4acc66d Primary data file for dataset ID 733238

Supplemental Files

File

Table of culture conditions for each of the 8 trials
filename: Dudgeon_seaweed_trial_meta_2018-01.pdf

(Portable Document Format (.pdf), 429.04 KB)
MD5:5a4a9cbd3cb5092a419b4883c96979b8

Notes: Trial 1 was a pilot test of culture system and methodological procedures so was not used for data collection in the testing of hypotheses. In each culture pot, pCO2 was set by the supply rate of CO2 in the corresponding mass-flow controlled gas mixing system to be within a target range of either near ambient, moderately elevated or highly elevated in each trial. The near-ambient range was narrower than the other target ranges (set points typically ~380 - 390 micro-atm) as it served as the control range in each trial. However, actual pCO2 in solution in each culture pot varied slightly from constant target values on a diurnal cycle associated with the metabolic activities of the algae contained within each pot. Unique average values of pCO2 in each culture pot based on different set values for each mass-flow controlled mixer within the qualitative ranges of ambient, moderate and highly increased pCO2 levels that were replicated in each trial of the experiment enabled a more powerful regression-type experimental design. With a regression type approach we could estimate the functional relationship between response variables and pCO2, which was not possible with a simple categorical treatment design.

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1316198

Deployments

None available yet

Instruments

Fluorometer

Supplied Name: Walz Underwater fluorometer - Diving-PAM, Heinz Walz, Effeltrich, Germany

Supplied Description:

Used to measure chlorophyll fluorescence

Instrument Type

Generic Name: Fluorometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/113/

Generic Description:

A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
date	date of data collection formatted as yyyy-mm-dd	unitless	date_local
F	Relative fluorescence of photosystem II	unitless	fluor
Fm	Maximum fluorescence	unitless	fluor
ID	Unique identifier for every sample; concatenation of trial_tank ID_plant ID_week	unitless	sample
light	Photosynthetically active radiation (PAR)	micromole photons/meter^2/second ( µmole photons . m^-2 . s^-1)	PAR
NPQ	Non-photochemical quenching normalized to Fm measured in the light	unitless	no_bcodmo_term
pCO2	Partial pressure of CO2 in solution in culture pot	atm	pCO2
Plant_ID	Plant ID number in culture pot	unitless	sample
qN	Non-photochemical quenching expressed as a fraction of difference between Fm and minimum F	unitless	no_bcodmo_term
qP	Photochemical quenching	unitless	no_bcodmo_term
rETR	Relative Electron Transport Rate	unitless	no_bcodmo_term
Tank_ID	Identifying number of culture pot	unitless	sample
Temp	Temperature of seawater in culture pot	degrees Celsius (°C)	temp
Trial	Trial number of the experiment	unitless	replicate
Week	Week number of given trial	unitless	time_point
Yield	Effective (or Optimal if dark-adapted) quantum yield. Calculated as Yield = (Fm - F)/Fm	unitless	Fv_Fm