Dataset: Plocamium growth and biomass

Name: Experimental results of Plocamium cartilagineum growth and biomass as a function of pCO2 and temperature (Seaweed OA Resilience project)
Published: 2018-03-23
Keywords: biota, oceans

Cite This Dataset

DOI:10.1575/1912/bco-dmo.726452.1

Spatial Extent: N:34 E:-118 S:33 W:-119

Temporal Extent: 2014-06-04 - 2015-02-23

Project:

Ocean Acidification: Scope for Resilience to Ocean Acidification in Macroalgae (Seaweed OA Resilience)

Program:

Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA) (SEES-OA)

Principal Investigator:

Janet E. Kubler (California State University Northridge, CSU-Northridge)

Co-Principal Investigator:

Steve Dudgeon (California State University Northridge, CSU-Northridge)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2018-03-23

Restricted:

Release Date:

2019-06-03

Validated:

Yes

Current State:

Final no updates expected

Data URL:

https://www.bco-dmo.org/dataset/726452/data

Experimental results of Plocamium cartilagineum growth and biomass as a function of pCO2 and temperature (Seaweed OA Resilience project)

Abstract:

Experimental results of Plocamium cartilagineum growth and biomass as a function of pCO2 and temperature.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2018-03-23	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.1575/1912/bco-dmo.726452.1	2019-06-03

Description

This dataset includes measurements of frond area of Plocamium cartilagineum grown under various temperatures and CO2 levels. Biomass-specific growth and dynamic average of weekly growth is also calculated.

Related Datasets:
Plocamium carbon nitrogen and stable isotopes: Plocamium carbon and nitrogen content and stable isotope values, 2014-2015 (Seaweed OA Resilience project)

Plocamium culture carbonate chemistry: Carbonate chemistry in experimental cultures of Plocamium cartilagineum cultured at different temperatures and pCO2 levels (Seaweed OA Resilience project)

Plocamium culture: seawater delta13C: Stable isotope ratio and concentration of carbon in seawater during Plocamium culture experiments, 2014-2015 (Seaweed OA Resilience project)

Plocamium cultures pH and temperature: Plocamium culture pot pH and temperature time-series at 10 minute sampling intervals from 2014-2015 (Seaweed OA Resilience project)

Plocamium exptl treatments summary: Summary of pCO2 and temperature treatment combinations for each culture pot and experimental trial (Seaweed OA Resilience project)

Plocamium pigments: Photosynthetic pigment concentrations in Plocamium cartilagineum, trials 3-8, 2014-2015 (Seaweed OA Resilience project)

Plocamium: pH drift: Carbonate chemistry over a time-course in pH drift experiments with Plocamium growth collected at Catalina Island, 2014-2015 (Seaweed OA Resilience project)

Rapid Light Curves_PAM: Measurements of fluorescence of photosystem II in Plocamium cartilagineum under various and pCO2 and temperature conditions

Acquisition Description

Plocamium cartilagineum was collected from Catalina Island in June - Nov. 2014 and Jan. 2015.

Culture pots were placed in large thermally insulated coolers in a temperature-controlled water bath at either 15 or 20°C under saturating illumination of ~150 µmoles photons/m^2/s. pCO2 treatments were supplied to closed culture pots by use of a gas mixing system combining Nitrogen, Oxygen and Carbon Dioxide to specific CO2 partial pressures, 20.9% oxygen and the balance being Nitrogen.

Areal growth: Frond pieces were placed under glass without overlap of branches adjacent to a ruler for scale and imaged using an Infinity 2 camera with attached macro lens mounted on a copy stand. Images and calibrated and surface area of fronds measured using Image J. Measurements were taken at the start and end of the trial. For all measures of growth (by surface area [adj_growth], and biomass [growth_biom] and [growth_dyn]) the calculations are specific growth rates, meaning LOG(measure@t2 / measure@t1)/(t2-t1). For surface area measures (adj_growth) and biomass (as growth_biom), t1 is the initial measure at the start of the experiment and t2 is the final measure at the end of the experiment. For biomass (asgrowth_dyn), t1 is the measure at the start of each weeklong interval and t2 is the measure at the end of the interval. Thus, there were typically 3 such intervals during a trial in which growth was measured and the datum in the column growth_dyn represents the average of those interval specific measures over the course of a trial.

Note: Since tagging individual fronds compromised frond performance, all fronds in a culture pot were treated as sub-samples that were summed to give the datum for the culture pot that was treated as the replicate.

Biomass: Frond pieces were gently blotted dry with paper toweling to remove surface water and weighed at weekly intervals to estimate biomass specific growth rates (growth_biom and growth_dyn). Note: since tagging individual fronds compromised frond performance, all fronds in a culture pot were treated as sub-samples that were summed to give the datum for the culture pot that was treated as the replicate.

Note: Trial 1 was a pilot test of culture system and methodological procedures so was not used for data collection in the testing of hypotheses.

See Supplemental Files for a table of culture conditions for each of the 8 trials (pdf).

Processing Description

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- combined data from 3 submitted files: CO2trt_x_pot_trial.csv, Plo_areal_growth_CO2_Temp.csv, Plo_bio_growth_CO2_Temp.csv
- added columns for date_start and date_end; moved trial to first column
- reduced precision of area and growth columns from 7 to 2 decimal places and adj_growth from 9 to 5 places.

Supplemental Files

File

Table of culture conditions for each of the 8 trials
filename: Dudgeon_seaweed_trial_meta_2018-01.pdf

(Portable Document Format (.pdf), 429.04 KB)
MD5:5a4a9cbd3cb5092a419b4883c96979b8

Notes: Trial 1 was a pilot test of culture system and methodological procedures so was not used for data collection in the testing of hypotheses. In each culture pot, pCO2 was set by the supply rate of CO2 in the corresponding mass-flow controlled gas mixing system to be within a target range of either near ambient, moderately elevated or highly elevated in each trial. The near-ambient range was narrower than the other target ranges (set points typically ~380 - 390 micro-atm) as it served as the control range in each trial. However, actual pCO2 in solution in each culture pot varied slightly from constant target values on a diurnal cycle associated with the metabolic activities of the algae contained within each pot. Unique average values of pCO2 in each culture pot based on different set values for each mass-flow controlled mixer within the qualitative ranges of ambient, moderate and highly increased pCO2 levels that were replicated in each trial of the experiment enabled a more powerful regression-type experimental design. With a regression type approach we could estimate the functional relationship between response variables and pCO2, which was not possible with a simple categorical treatment design.

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1316198

Deployments

None available yet

Instruments

Aquarium chiller

Supplied Name: Aqua Logic Cyclone Chiller

Supplied Description:

Used to control the temperature of the growth chambers.

Instrument Type

Generic Name: Aquarium chiller

Generic Description:

Immersible or in-line liquid cooling device, usually with temperature control.

Camera

Supplied Name: Lumenera Corp. Infinity 2 2.0 Megapixel Color CCD Camera, Navitar TV Zoom 7000 Macro lens

Supplied Description:

To take images of fronds for area measurements.

Instrument Type

Generic Name: Camera

Acronym: camera

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/311/

Generic Description:

All types of photographic equipment including stills, video, film and digital systems.

Mass Flow Controller

Supplied Name: Qubit Systems Mass Flow Controllers (MFC)

Supplied Description:

Used to mix gasses: Nitrogen, 1 L/min; Oxygen, 250 ml/min; CO2, 2 ml/min.

Instrument Type

Generic Name: Mass Flow Controller

Acronym: MFC

Generic Description:

Mass Flow Controller (MFC) - A device used to measure and control the flow of fluids and gases

Scale

Supplied Name: Mettler Toledo AG204 Delta Range Analytical Balance

Supplied Description:

To measure biomass of fronds

Instrument Type

Generic Name: Scale

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB13/

Generic Description:

An instrument used to measure weight or mass.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
pot	culture pot identifier	unitless	sample
pCO2	partial pressure of dissolved CO2	microatmospheres (uatm)	pCO2
temp	Temperature	degrees Celsius	temp
trial	experimental trial consisting of 3 PCO2 levels with one always ~380 as control	unitless	exp_id
date_start	start date for trial formatted as yyyy-mm-dd	unitless	date_start
date_end	end date for trial formatted as yyyy-mm-dd	unitless	date_end
area_t0	frond surface area on day 0 (start)	square centimeters (cm^2)	surface_area
area_end	frond surface area at expt. end	square centimeters (cm^2)	surface_area
days	Number of days for experimental trial	days	days
adj_growth	Area-specific growth rate	square centimeters/square centimeters/day (cm^2/cm^2/day)	growth
growth_biom	biomass-specific growth between t=0 and t=19 or 20 or 21 days	milligrams/gram/day (mg/g/day)	growth
growth_dyn	dynamic average of weekly growth rates over the 3-4 week experiment	milligrams/gram/day (mg/g/day)	growth
pCO2_target_range	Relative range for the target pCO2. See Supplemental Files for the table of culture conditions for each of the 8 trials.	unitless	pCO2