Dataset: AE1812 Alkaline phosphatase

Name: Alkaline phosphatase activities for in situ and incubation samples from RV/Atlantic Explorer cruise AE1812 cruise transect from Bermuda to Rhode Island in May 2018.
Published: 2018-07-16
Keywords: Alkaline phosphatase, diatoms, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.739973.1

Spatial Extent: N:40.42 E:-56.56 S:31.42 W:-70.58

Temporal Extent: 2018-05-02 - 2018-05-15

Project:

Collaborative Research: Dynamics of dissolved organic phosphorus production, composition and bioavailability along a natural marine phosphate gradient (DOP Dynamics-N Atl)

Collaborative Research: Defining the biogeochemical drivers of diatom physiological ecology in the North Atlantic (North Atlantic Diatoms)

Principal Investigator:

Sonya T. Dyhrman (Lamont-Doherty Earth Observatory, LDEO)

Co-Principal Investigator:

Bethany D. Jenkins (University of Rhode Island, URI-GSO)

Tatiana A. Rynearson (University of Rhode Island, URI-GSO)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2018-07-16

Restricted:

Release Date:

2020-06-29

Validated:

Yes

Current State:

Final no updates expected

Alkaline phosphatase activities for in situ and incubation samples from RV/Atlantic Explorer cruise AE1812 cruise transect from Bermuda to Rhode Island in May 2018.

Abstract:

This dataset reports alkaline phosphatase activities (APA) for 3 incubation runs and 33 in situ samples collected on RV/Atlantic Explorer cruise AE1812 in May 2018. The samples were collected between Bermuda and Rhode Island.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2018-07-16	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.739973.1	2020-06-29

Description

Methods & Sampling

For APA analysis, triplicate biological samples (250 mL) from in situ and incubation samples were filtered onto 47-mm polycarbonate membranes (0.2 μm). Stored at −20°C until analysis.

APA was assayed after Dyhrman and Ruttenberg (2006) using the fluorogenic phosphatase substrate 6,8-difluoro-4-methylumbelliferyl phosphate. Values were normalized to both volume and chl a. Reagents/Abs/Em used:

D-6567 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP):
- Storage upon receipt: ≤ 20°C; Desiccate
- Abs/Em = 358/455
- Molecular Formula: C10H7F2O6P
- Molecular Weight: 292.1
- CAS Name/Number: 2H-1-Benzopyran-2-one, 6,8-difluoro-4-methyl-7-(phosphonooxy)-/ 214491-43-7

D-6566 6,8-difluoro-7-Hydroxy-4-Methylcoumarin (DiFMU) - Reference Standard:
- Storage upon receipt: Room temp.; protect from light
- Molecular Formula: C10H6F2O3
- Molecular Weight: 212.15
- CAS Name/Number: 2H-1-Benzopyran-2-one, 6,8-difluoro-7-hydroxy-4-methyl-/ 215868-23-8

Incubation key:
Control = no addition of nutrients or deep water
DSW = deep seawater addition (added 20% deep seawater (700 m))
+P = Added phosphate only (0.5 µM final for incubations 1 and 2, 1 µM final for incubation 3)
+N = Added nitrate only (6 µM final for incubations 1 and 2, 12 µM final for incubation 3)
phi_P = All but P added (N, Si, Fe, B12)
phi_N = All but N added (P, Si, Fe, B12)
-1, -2, -3 = biological replicates

In situ key:
IS = in situ
-1, -2, -3 = biological replicates

Lost = sample was lost

Data Processing Description

BCO-DMO Processing:
-combined 3 incubations and in situ data into one dataset.
-decreased number of decimal places for APA from various to 3.
-changed date format from m/d/yyyy to yyyy-mm-dd
-changed sample name phi symbol to text

Data Files

File
APA.csv (Comma Separated Values (.csv), 4.71 KB) MD5:a85b927a7e2a67cf8b063e5553870489 Primary data file for dataset ID 739973

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1558506
NSF Division of Ocean Sciences	OCE-1756337
NSF Division of Ocean Sciences	OCE-1756964

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
AE1812		2018-05-01	R/V Atlantic Explorer	Tatiana A. Rynearson (Chief Scientist)	data info

Instruments

plate reader

Supplied Name: Biotek Synergy fluorescent plate reader

Supplied Description:

Samples were run on a Biotek Synergy fluorescent plate reader using black plates

Instrument Type

Generic Name: plate reader

Generic Description:

Plate readers (also known as microplate readers) are laboratory instruments designed to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 6-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 uL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. From: http://en.wikipedia.org/wiki/Plate_reader, 2014-09-0-23.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
cast	Cast number on cruise	unitless	cast
date_harvest	Day on which samples were filtered and stored; formatted as yyyy-mm-dd	unitless	date
lat	latitude; north is positive	decimal degrees	lat
lon	longitude; east is positive	decimal degrees	lon
APA_nmolP_hr_liter	Alkaline phosphatase activity; volume normalized	nanomol Phosphate/hour/liter [nmol P/h/L]	no_bcodmo_term
APA_nmolP_hr_ug_chla	Alkaline phosphatase activity; chl a normalized	nanomol Phosphate/hour/microgram chlorophyll-a [nmol P/h/µg Chl a]	no_bcodmo_term
incubation	Incubation replicate or in situ sampling	unitless	replicate
sample	Sample identifier	unitless	sample
station	Station identification number	unitless	station