Dataset: Mesocosm Growth Data Winter 2017

Name: Size of B. neritina colonies with and without symbiont grown at different temperatures
Published: 2018-10-18
Keywords: biota, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.748480.1

Spatial Extent: N:37.3 E:-75.9 S:34.7 W:-76.8

Project:

Biogeography of a marine defensive microbial symbiont: relative importance of host defense vs. abiotic factors (BiogeogDefensiveSymb)

Principal Investigator:

Nicole Lopanik (Georgia Institute of Technology, GA Tech)

Co-Principal Investigator:

Grace Lim-Fong (Georgia Institute of Technology, GA Tech)

BCO-DMO Data Manager:

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2018-10-18

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Size of B. neritina colonies with and without symbiont grown at different temperatures

Abstract:

This dataset includes sizes of B. neritina colonies with and without symbiont grown at different temperatures.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2018-10-18	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.748480.1	2020-11-25

Methods & Sampling

B. neritina colonies were collected by hand off the sides of floating docks around Morehead City, NC, and Oyster, VA. Larvae from individual colonies were collected and settled into 10 mL of filtered seawater with (treated) or without (control) 100 ug/mL of gentamicin as described in Lopanik et al. 2004. Larvae were allowed to settle overnight. The control and treated seawater was changed the next morning for the next 3 days. The plates were transported to Georgia Tech where they were placed in 2.5 gallon tanks with artificial seawater maintained in environmental chambers at 16°C and 22°C either with or without 2 invertebrate predators (tunicates). They were fed ad libitum an artificial diet of the phytoplankton Rhodomonas lens for approximately three months. After a 5 week period, the size of the colonies was measured by counting the number of zooids in each colony. Samples of larvae, juvenile and the grown colonies were placed in RNAlater for symbiont quantification analysis by quantitative PCR. After 13 and 25 weeks, the size of the colonies was measured by counting the number of bifurcations, and colonies were placed into RNAlater for symbiont quantification. Data was analyzed using IBM SPSS Statistics 24.

Data Processing Description

BCO-DMO Processing: modified parameter names to conform with BCO-DMO naming conventions (replaced spaces with underscores, replaced "+" with "plus", replaced "-" with "minus", replaced "+SE" with "SE").

Data Files

File
mesocosm_winter_17.csv (Comma Separated Values (.csv), 1.94 KB) MD5:c9504246a122163cbc5122bd51cc3502 Primary data file for dataset ID 748480

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1608709

Deployments

None available yet

Instruments

Aquarium

Supplied Name: tank

Supplied Description:

Instrument Type

Generic Name: Aquarium

Generic Description:

Aquarium - a vivarium consisting of at least one transparent side in which water-dwelling plants or animals are kept

Microscope - Optical

Supplied Name: Leica stereomicroscope

Supplied Description:

A Leica stereomicroscope was used while counting the number of colony bifurcations.

Instrument Type

Generic Name: Microscope - Optical

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/

Generic Description:

Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".

qPCR Thermal Cycler

Supplied Name: quantitative PCR

Supplied Description:

Instrument Type

Generic Name: qPCR Thermal Cycler

Acronym: qPCR

Generic Description:

An instrument for quantitative polymerase chain reaction (qPCR), also known as real-time polymerase chain reaction (Real-Time PCR).

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Nplus_Ant_SE_5w	Std. error of avg. size in N+ antibiotic treated colonies after N/A	unitless	count
Nplus_Cont_N_13w	Number of colonies in which size was measured	unitless	count
Nplus_Cont_Avg_25w	Avg size in bifurcs of N+ cont colonies after 25 weeks	unitless	count
Nplus_Ant_N_5w	Number of colonies in which size was measured	unitless	count
Nplus_Ant_Avg_13w	Avg size in bifurcs of N+ antibiotic treated colonies after 13 weeks N/A	unitless	count
Nplus_Cont_SE_25w	Std. error of avg. size in N+ cont colonies	unitless	count
Nminus_Cont_Avg_5w	Avg size in zooids of N- cont colonies after 5 weeks	unitless	count
Nplus_Ant_SE_13w	Std. error of avg. size in N+ antibiotic treated colonies	unitless	count
Nplus_Cont_N_25w	Number of colonies in which size was measured	unitless	count
Nminus_Cont_SE_5w	Std. error of avg. size in N- cont colonies	unitless	count
Nplus_Ant_N_13w	Number of colonies in which size was measured	unitless	count
Nplus_Ant_Avg_25w	Avg size in bifurcs of N+ antibiotic treated colonies after 25 weeks N/A	unitless	count
Nminus_Cont_N_5w	Number of colonies in which size was measured	unitless	count
Nminus_Cont_Avg_13w	Avg size in bifurcs of N+ cont colonies after 13 weeks	unitless	count
Nplus_Ant_SE_25w	Std. error of avg. size in N+ antibiotic treated colonies	unitless	count
Nminus_Ant_Avg_5w	Avg size in zooids of N- antibiotic treated colonies after 5 weeks	unitless	count
Nminus_Cont_SE_13w	Std. error of avg. size in N+ cont colonies	unitless	count
Nplus_Ant_N_25w	Number of colonies in which size was measured	unitless	count
Tank_Tmt	Mesocosm treatment (Cold or Warm, With or without predator)	unitless	treatment
Nminus_Ant_SE_5w	Std. error of avg. size in N- antibiotic treated colonies	unitless	count
Nminus_Cont_N_13w	Number of colonies in which size was measured	unitless	count
Nminus_Cont_Avg_25w	Avg size in bifurcs of N+ cont colonies after 25 weeks	unitless	count
Splus_Cont_Avg_5w	Avg size in zooids of S+ cont colonies after 5 weeks	unitless	count
Splus_Cont_SE_5w	Std. error of avg. size in S+ cont colonies	unitless	count
Nminus_Ant_N_5w	Number of colonies in which size was measured	unitless	count
Nminus_Ant_Avg_13w	Avg size in bifurcs of N+ antibiotic treated colonies after 13 weeks N/A	unitless	count
Nminus_Cont_SE_25w	Std. error of avg. size in N+ cont colonies	unitless	count
Splus_Cont_N_5w	Number of colonies in which size was measured	unitless	count
Splus_Cont_Avg_13w	Avg size in bifurcations of S+ cont colonies after 13 weeks N/A	unitless	count
Nminus_Ant_SE_13w	Std. error of avg. size in N+ antibiotic treated colonies	unitless	count
Nminus_Cont_N_25w	Number of colonies in which size was measured	unitless	count
Splus_Ant_Avg_5w	Avg size in zooids S+ antibiotic treated colonies after 5 weeks	unitless	count
Splus_Cont_SE_13W	Std. error of avg. size in S+ cont colonies	unitless	count
Nminus_Ant_N_13w	Number of colonies in which size was measured	unitless	count
Nminus_Ant_Avg_25w	Avg size in bifurcs of N+ antibiotic treated colonies after 25 weeks N/A	unitless	count
Splus_Ant_SE_5w	Std. error of avg. size in S+ antibiotic treated colonies	unitless	count
Splus_Cont_N_13w	Number of colonies in which size was measured	unitless	count
Splus_Cont_Avg_25w	Avg size in bifurcations of S+ cont colonies after 25 weeks N/A	unitless	count
Nminus_Ant_SE_25w	Std. error of avg. size in N+ antibiotic treated colonies	unitless	count
Splus_Ant_N_5w	Number of colonies in which size was measured	unitless	count
Splus_Ant_Avg_13w	Avg size in bifurcs S+ antibiotic treated colonies after 13 weeks	unitless	count
Splus_Cont_SE_25w	Std. error of avg. size in S+ cont colonies	unitless	count
Nminus_Ant_N_25w	Number of colonies in which size was measured	unitless	count
Nplus_Cont_Avg_5w	Avg size in zooids of N+ cont colonies after 5 weeks	unitless	count
Splus_Ant_SE_13w	Std. error of avg. size in S+ antibiotic treated colonies	unitless	count
Splus_Cont_N_25w	Number of colonies in which size was measured	unitless	count
Nplus_Cont_SE_52	Std. error of avg. size in N+ cont colonies	unitless	count
Splus_Ant_N_13w	Number of colonies in which size was measured	unitless	count
Splus_Ant_Avg_25w	Avg size in bifurcs S+ antibiotic treated colonies after 25 weeks	unitless	count
Nplus_Cont_N_5w	Number of colonies in which size was measured	unitless	count
Nplus_Cont_Avg_13w	Avg size in bifurcs of N+ cont colonies after 13 weeks	unitless	count
Splus_Ant_SE_25W	Std. error of avg. size in S+ antibiotic treated colonies	unitless	count
Nplus_Ant_Avg_5w	Avg size in zooids of N+ antibiotic treated colonies after 5 weeks	unitless	count
Nplus_Cont_SE_13w	Std. error of avg. size in N+ cont colonies	unitless	count
Splus_Ant_N_25w	Number of colonies in which size was measured	unitless	count

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Dataset: Mesocosm Growth Data Winter 2017