Dataset: HHQ Chlorophyll

Name: Chlorophyll measurements from HHQ experiments conducted during the MesoHux mesocosm experiment, May 2017, Bergen, Norway
Published: 2019-01-23
Keywords: oceans

Cite This Dataset

DOI:10.1575/1912/bco-dmo.753388.1

Spatial Extent: N:60.221 E:5.281 S:60.221 W:5.281

Temporal Extent: 2017-05-16 - 2017-05-31

Project:

Collaborative Research: Building a framework for the role of bacterial-derived chemical signals in mediating phytoplankton population dynamics (HHQSignals)

Principal Investigator:

Elizabeth Harvey (Skidaway Institute of Oceanography, SkIO)

Co-Principal Investigator:

David Rowley (University of Rhode Island, URI)

Kristen E. Whalen (Haverford College)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2019-01-23

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Chlorophyll measurements from HHQ experiments conducted during the MesoHux mesocosm experiment, May 2017, Bergen, Norway

Abstract:

This dataset includes chlorophyll measurements from HHQ experiments conducted during the MesoHux mesocosm experiment, May 2017, Bergen, Norway. Microbial mesocosms were spiked with 2-heptyl-4-quinolone (HHQ).

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2019-01-23	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.1575/1912/bco-dmo.753388.1	2019-03-14

Description

Methods & Sampling

Water samples for chlorophyll extraction were collected either from the mesocosms via a 5 L Niskin or subsampled from experimental bottles. Chlorophyll samples were filtered in triplicate through a 25mm Glass Fiber Filter (GFF), and immediately extracted in 6 mL of ethanol for 12-18 hours in the dark at room temperature. After extraction, filters were removed from the sample, and the fluorescence of the sample was read on a Turner AU10. The sample was then acidified with 1 drop of 10% HCL and re-read on the same instrument. The fluorometer was calibrated prior to using with a chlorophyll standard purchased from Sigma.

Mesocosm treatment for all HHQ experiments was as follows:
Redfield: N:P added in a 16:1 ratio during the first 3 days of the experiment, no shading

HHQ treatments here are as follows:
High HHQ - 100 ng mL-1 (410 uM) added to triplicate 5L bottles.
DMSO control - equivalent (v:v) DMSO added to triplicate 5L bottles.

All bottles were incubated for 24h in a flow-through tank, that was shaded to mimic in situ conditions. Chlorophyll samples were taken at T0 and T24 for all experiments.

Data were processed in Excel with statistics run in Excel, R, or Matlab.

Data Processing Description

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- reduced precision of total chlorophyll columns from (5 to 15) to 2 decimal places

Data Files

File
chlorophyll.csv (Comma Separated Values (.csv), 4.77 KB) MD5:28df4bedc5ac8fc7d022a8513b0541d3 Primary data file for dataset ID 753388

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1657898

Deployments

None available yet

Instruments

Fluorometer

Supplied Name: Turner AU10 fluorometer

Supplied Description:

Instrument Type

Generic Name: Fluorometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/113/

Generic Description:

A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.

Niskin bottle

Supplied Name: 5 L Niskin

Supplied Description:

Used to collect water samples.

Instrument Type

Generic Name: Niskin bottle

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/

Generic Description:

A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Time	time since start of experiment	hours	time_inc
Total_chl_with_phaeo	total chlorophyll including phaeophytin	micrograms chlorophyll/Liter (ug/L)	chl_tot
Total_Chl_no_phaeo	total chlorophyll NOT including phaeophytin	micrograms chlorophyll/Liter (ug/L)	no_bcodmo_term
Extract_Volume_mL	volume extracted	milliliters (mL)	volume
Volume_Filtered_mL	volume filtered	milliliters (mL)	fluor_vol_filt
F_o	initial fluorescence reading	Relative Fluorescence Units (RFU)	fluor
F_o_blank	initial fluorescence of control blank	Relative Fluorescence Units (RFU)	fluor
F_a	fluorescence after acidification	Relative Fluorescence Units (RFU)	fluor
F_a_blank	fluorescence of control blank after acidification	Relative Fluorescence Units (RFU)	fluor
Dilution_Factor	dilution factor	unitless	dilution
Date	sampling date formatted as Mon dd yyyy	unitless	date
Sample	sample identifier	unitless	sample
Experiment_num	experiment number	unitless	exp_id
Replication	replicate number	unitless	replicate

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Dataset: HHQ Chlorophyll

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Dataset: HHQ Chlorophyll

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