Dataset: Breviolum antillogorgium cell counts

Name: Cell counts of Breviolum antillogorgium cultures isolated from the octocoral Antillogorgia bipinnata by sample and treatment for cultures historically grown at 26C and 30C and reciprocally grown at 26C and 30C, Oct - Dec 2018
Published: 2019-02-20
Keywords: Breviolum antillogorgium, symbiont growth, biota, oceans

Cite This Dataset

DOI:10.1575/1912/bco-dmo.756510.1

Spatial Extent: N:25.1326 E:-80.2635 S:25.1326 W:-80.2635

Temporal Extent: 2018-10-02 - 2018-12-24

Project:

RUI: Collaborative Research: Genetic variation as a driver of host and symbiont response to increased temperature on coral reefs (Host Symbiont Temp Response)

Principal Investigator:

Mary Alice Coffroth (State University of New York at Buffalo, SUNY Buffalo)

Co-Principal Investigator:

Casey terHorst (California State University Northridge, CSUN)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2019-02-20

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Cell counts of Breviolum antillogorgium cultures isolated from the octocoral Antillogorgia bipinnata by sample and treatment for cultures historically grown at 26C and 30C and reciprocally grown at 26C and 30C, Oct - Dec 2018

Abstract:

Cell counts of Breviolum antillogorgium cultures isolated from the octocoral Antillogorgia bipinnata by sample and treatment for cultures historically grown at 26 and 30 degrees C and reciprocally grown at 26 and 30 degrees C

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2019-02-20	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.1575/1912/bco-dmo.756510.1	2019-03-04

Description

Cell counts of Breviolum antillogorgium cultures isolated from the octocoral Antillogorgia bipinnata by sample and treatment for cultures historically grown at 26 and 30 degrees C and reciprocally grown at 26 and 30 degrees C.

Methods & Sampling

Breviolum antillogorgium cultures were initially isolated from Antillogorgia bipinnata colonies collected at in the upper Florida Reef tract at Elbow Reef (N 25 07.956 W 80 15.810) in September 2016. Culture isolations and cell growth experimentation occurred in the Coffroth lab, University at Buffalo.

A total of six Breviolum antillogorgium genotypes were used in this experiment. Three cultures had been grown at 26 degrees C since isolation in 2016 (16-0590F, 16-0875 and 16-1631) and three had been grown at 30 degrees C since isolation in 2016 (16-0587, 16-0763 and 16-0764). Three replicates of each of the six Breviolum antillogorgium cultures were grown at 26 and 30 C for a total of four treatments (26 degrees at 26 and 30 C and 30 degrees C at 26 and 30 C). A total of four to six replicate counts from each culture was made every three to twelve days using light microscopy and a hemocytometer. Mean symbiont density per ml was calculated.

Data Processing: Mean x 10000 were calculated from the counts.

Data Processing Description

BCO-DMO Processing notes:- created separate files for each daily cell count
- added columns for Count_5 and Count_6 to data from day 56 to day 83 to match days 1 to 55
- created a toplevel file with date and day from each Excel sheet, which calls the daily files

Data Files

File
cell_counts_growth.csv (Comma Separated Values (.csv), 31.10 KB) MD5:77fbfe3947021f159b8238e8dbc08033 Primary data file for dataset ID 756510

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1559286

Deployments

None available yet

Instruments

Hemocytometer

Supplied Name: Reichert Brightline hemocytometer

Supplied Description:

Used to make cell counts.

Instrument Type

Generic Name: Hemocytometer

Generic Description:

A hemocytometer is a small glass chamber, resembling a thick microscope slide, used for determining the number of cells per unit volume of a suspension. Originally used for performing blood cell counts, a hemocytometer can be used to count a variety of cell types in the laboratory. Also spelled as "haemocytometer". Description from:
http://hlsweb.dmu.ac.uk/ahs/elearning/RITA/Haem1/Haem1.html.

Microscope - Optical

Supplied Name: Zeiss

Supplied Description:

Instrument Type

Generic Name: Microscope - Optical

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/

Generic Description:

Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Date	date of count formatted as yyyy-mm-dd	unitless	date
Day	Number of days since inoculation	days	days
Culture_ID	Identification of sample given as culture name (16-0587; 16-0590F; 16-1631; etc.); treatment temperature (26 or 30 C) and replicate (1; 2; 3)	unitless	sample
Count_1	First replicate of culture counted	cells x 10^4/milliliter (cells x 10^4 ml-1)	cell_concentration
Count_2	Second replicate of culture counted	cells x 10^4/milliliter (cells x 10^4 ml-1)	cell_concentration
Count_3	Third replicate of culture counted	cells x 10^4/milliliter (cells x 10^4 ml-1)	cell_concentration
Count_4	Fourth replicate of culture counted	cells x 10^4/milliliter (cells x 10^4 ml-1)	cell_concentration
Count_5	Fifth replicate of culture counted	cells x 10^4/milliliter (cells x 10^4 ml-1)	cell_concentration
Count_6	Sixth replicate of culture counted	cells x 10^4/milliliter (cells x 10^4 ml-1)	cell_concentration
Mean	Mean of replicate counts	cells x 10^4/milliliter (cells x 10^4 ml-1)	cell_concentration