Detailed methods can be found in McCormick, et al., 2019.

Briefly, the time series test recorded electroretinogram (ERG) responses to a 1 s square step of light at a constant irradiance of 3.56 μmol photons m−2 s−1 repeated every 20 s, providing a nearly continuous measure of ERG response in a tethered, live larva during the experimental manipulation of partial pressure of oxygen (pO2). There was a constant flow of pH-buffered sterile seawater in the chamber where the larva was held, and after a brief period in “normoxia” (surface-ocean oxygen levels), the pO2 was decreased, and then held at a low pO2 before re-oxygenating the solution. This dataset shows additional experimental details for the “TimeSeries” and “OxygenMetrics_Vision” datasets, including the original experiment name (that can be matched with the experiment name from the other datasets), the time periods for each oxygen exposure, and the pH and temperature of the experiments.

Oxygen was measured using a Microx4 (PreSens) oxygen meter and a Pst-7 oxygen optode probe.