Dataset: AE1812 incubation Chl a

Name: Chlorophyll a concentrations from addition incubation experiments performed aboard AE1812 while transecting from the Sargasso Sea to Coastal Rhode Island in May 2018.
Published: 2020-08-14
Keywords: chlorophyll a, subsurface chlorophyll maximum, in situ samples, on deck incubations, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.820938.1

Spatial Extent: N:41.193917 E:-63.2384 S:31.684933 W:-70.96835

Temporal Extent: 2018-05-07 - 2018-05-15

Project:

Collaborative Research: Defining the biogeochemical drivers of diatom physiological ecology in the North Atlantic (North Atlantic Diatoms)

Lead Principal Investigator:

Tatiana A. Rynearson (University of Rhode Island, URI-GSO)

Co-Principal Investigator:

Bethany D. Jenkins (University of Rhode Island, URI-GSO)

BCO-DMO Data Manager:

Christina Haskins (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2020-08-14

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Chlorophyll a concentrations from addition incubation experiments performed aboard AE1812 while transecting from the Sargasso Sea to Coastal Rhode Island in May 2018.

Abstract:

Chlorophyll a concentrations from addition incubation experiments performed aboard AE1812 while transecting from the Sargasso Sea to Coastal Rhode Island in May 2018.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2020-08-14	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.820938.1	2020-08-26

Methods & Sampling

Chlorophyll a was measured at the conclusion of each incubation experiment conducted in triplicate on AE1812. Samples were taken from biological replicates and filtered onto GF/F, 20 µm, 5µm polycarbonate filters.

Chl a was extracted from filters in 100% denatured ethanol for 12 hours, measured on a 10-AU Fluorometer, and chlorophyll a and phaeophytin concentrations were calculated based on a predetermined calibration curve. Concentrations on each filter were used to determine total chl a within each size fraction.

Data Processing Description

Data was processed in R 3.6.2 (R-Core-Team 2019)

BCO-DMO Data Manager Processing Notes:
* added a conventional header with dataset name, PI name, version date
* modified parameter names to conform with BCO-DMO naming conventions
* blank values in this dataset are displayed as "nd" for "no data." nd is the default missing data identifier in the BCO-DMO system.
* converted datetime into ISO_DateTime_UTC format
* set types for each data column

Data Files

File
chla_incub.csv (Comma Separated Values (.csv), 9.68 KB) MD5:c18996f801454420f457c753e86dd4d9 Primary data file for dataset ID 820938

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1558490

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
AE1812		2018-05-01	R/V Atlantic Explorer	Tatiana A. Rynearson (Chief Scientist)	data info

Instruments

Turner Designs Fluorometer 10-AU

Supplied Name: 10AU Fluorometer (Turner Designs, San Jose, CA)

Supplied Description:

Instrument Type

Generic Name: Turner Designs Fluorometer 10-AU

Acronym: Turner Fluorometer 10-AU

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0393/

Generic Description:

The Turner Designs 10-AU Field Fluorometer is used to measure Chlorophyll fluorescence. The 10AU Fluorometer can be set up for continuous-flow monitoring or discrete sample analyses. A variety of compounds can be measured using application-specific optical filters available from the manufacturer. (read more from Turner Designs, turnerdesigns.com, Sunnyvale, CA, USA)

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
ISO_DateTime_UTC	Date and time of incubation conclusion	YYYY-MM-DDTHH:MM:SS[.xx]Z	ISO_DateTime_UTC
experiment	Experiment number in series	unitless	exp_id
treatment	Experimental treatment	unitless	treatment
bottle_rep	Biological replicate of triplicate incubations	unitless	replicate
size_fraction	size fraction analyzed based on pore size of filter	m	no_bcodmo_term
vol_filtered	Volume of seawater filtered for chl a analysis	ml	vol_filt
vol_extracted	Volume of ethanol used to extract chl a	ml	sample_volume
Fb	Fluorescence before acidification	RFU	fluor
Fa	Fluorescence after acidification	RFU	fluor
blank_subt_Fb	Blank corrected fluorescence before acidification	RFU	instrument_blank
blank_subt_Fa	Blank corrected fluorescence after acidification	RFU	instrument_blank
chla	Chlorophyll a concentration	g/L	chl_a
phaeo	Phaeophytin concentration	g/L	phaeo

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Database

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Dataset: AE1812 incubation Chl a