Dataset: March Incubation Microscopy Cell Counts

Name: Microscopy cell counts from multivariate mesocosm experiments conducted with a natural phytoplankton community from Narragansett Bay, RI
Published: 2021-04-14
Keywords: Multiple drivers, environmental filtering, phytoplankton physiology, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.848977.1

Spatial Extent: N:41.57 E:-71.39 S:41.57 W:-71.39

Temporal Extent: 2017-03-20

Project:

Dimensions: Collaborative Research: Genetic, functional and phylogenetic diversity determines marine phytoplankton community responses to changing temperature and nutrients (Phytoplankton Community Responses)

Program:

Dimensions of Biodiversity (Dimensions of Biodiversity)

Principal Investigator:

Stephanie I. Anderson (University of Rhode Island, URI-GSO)

Co-Principal Investigator:

Gayantonia Franzè (Institute of Marine Research (Bergen, Norway), IMR)

David A. Hutchins (University of Southern California, USC)

Joshua D. Kling (University of California-Berkeley, UC Berkeley)

Colin T. Kremer (University of California-Los Angeles, UCLA)

Elena Litchman (Michigan State University, MSU)

Susanne Menden-Deuer (University of Rhode Island, URI-GSO)

Tatiana A. Rynearson (University of Rhode Island, URI-GSO)

Paul Wilburn (Michigan State University, MSU)

Contact:

Stephanie I. Anderson (University of Rhode Island, URI-GSO)

BCO-DMO Data Manager:

Taylor Heyl (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2021-04-14

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Microscopy cell counts from multivariate mesocosm experiments conducted with a natural phytoplankton community from Narragansett Bay, RI

Abstract:

This dataset represents microscopy cell counts from multivariate mesocosm experiments conducted with a natural phytoplankton community from Narragansett Bay, RI. These data were assessed in Anderson et al. The Interactive Effects of Temperature and Nutrients on a Spring Phytoplankton Community (in prep).

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2021-04-14	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.848977.1	2021-04-28

Methods & Sampling

Multivariate mesocosm experiments were conducted with a natural phytoplankton community from Narragansett Bay, RI. Water was incubated in triplicate at -0.5ºC, 2.6ºC, and 6ºC for 10 days. At each temperature, treatments included both nutrient amendments (N, P, Si addition) and controls (no macronutrients added).

At the onset and conclusion of the incubation experiments, the microplankton community was identified and quantified to discern treatment effects on community composition. Aliquots from each incubation were fixed in triplicate with 2% acid Lugol’s solution for microscopy, final concentration. Cell enumeration was conducted by performing cell counts ~>10 micrometer (µm) on a 1 milliliter (ml) Sedgewick cell-counting chamber (Structure Probe Inc.) using an Eclipse E800 microscope (Nikon).

These data were assessed as part of “The Interactive Effects of Temperature and Nutrients on a Spring Phytoplankton Community” (Anderson et al, in prep).

Data Processing Description

BCO-DMO processing description:

- Adjusted field/parameter names to comply with database requirements
- Missing data identifier ‘NA’ and ‘N/A’ replaced with 'nd' (BCO-DMO's default missing data identifier)
- Added a conventional header with dataset name, PI names, version date

Data Files

File
microscopy_cell_counts.csv (Comma Separated Values (.csv), 5.69 KB) MD5:93c4d6bf74809c9dad3b258ccb8d7864 Primary data file for dataset ID 848977

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1638834

Deployments

None available yet

Instruments

Microscope - Optical

Supplied Name: Eclipse E800 microscope (Nikon)

Supplied Description:

Instrument Type

Generic Name: Microscope - Optical

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/

Generic Description:

Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Treatment	Treatment identification. Letter corresponds to temperature treatment, L=low (-5_C), M=medium (2.6_C), H=high (6_C); and symbol describes whether macronutrients were added (+) or not (-). T0 is initial community.	days	treatment
Replicate	Biological replicate identification	unitless	replicate
Temperature	Nutrient treatment in which incubation was conducted	degrees celcius (¡C)	temp
Nutrient	Nutrient treatment in which incubation was conducted	unitless	treatment
Total_abundance	Total cell abundance	cells per liter (cells/L)	abundance
Alexandrium_sp	Species abundance	cells per liter (cells/L)	abundance
Asterionellopsis_glacialis	Species abundance	cells per liter (cells/L)	abundance
Bacteriastrum_sp	Species abundance	cells per liter (cells/L)	abundance
Cerataulina_pelagica	Species abundance	cells per liter (cells/L)	abundance
Chaetoceros_affinis	Species abundance	cells per liter (cells/L)	abundance
Chaetoceros_atlanticus	Species abundance	cells per liter (cells/L)	abundance
Chaetoceros_compressus	Species abundance	cells per liter (cells/L)	abundance
Chaetoceros_costatus	Species abundance	cells per liter (cells/L)	abundance
Chaetoceros_danicus	Species abundance	cells per liter (cells/L)	abundance
Chaetoceros_debilis	Species abundance	cells per liter (cells/L)	abundance
Chaetoceros_decipiens	Species abundance	cells per liter (cells/L)	abundance
Chaetoceros_diadema	Species abundance	cells per liter (cells/L)	abundance
Chaetoceros_didymus	Species abundance	cells per liter (cells/L)	abundance
Chaetoceros_laciniosus	Species abundance	cells per liter (cells/L)	abundance
Chaetoceros_mitra	Species abundance	cells per liter (cells/L)	abundance
Chaetoceros_similis	Species abundance	cells per liter (cells/L)	abundance
Chaetoceros_spp	Species abundance	cells per liter (cells/L)	abundance
Chaetoceros_subtilis	Species abundance	cells per liter (cells/L)	abundance
Chaetoceros_winghamii	Species abundance	cells per liter (cells/L)	abundance
Coscinodiscus_spp	Species abundance	cells per liter (cells/L)	abundance
Cylindrotheca_closterium	Species abundance	cells per liter (cells/L)	abundance
Dactyliosolen_blavyanus	Species abundance	cells per liter (cells/L)	abundance
Dactyliosolen_fragilissimus	Species abundance	cells per liter (cells/L)	abundance
Diatom_unknown	Species abundance	cells per liter (cells/L)	abundance
Dictyocha_speculum	Species abundance	cells per liter (cells/L)	abundance
Dinoflagellates_unknown	Species abundance	cells per liter (cells/L)	abundance
Ditylum_brightwellii	Species abundance	cells per liter (cells/L)	abundance
Flagellate_unknown	Species abundance	cells per liter (cells/L)	abundance
Guinardia_delicatula	Species abundance	cells per liter (cells/L)	abundance
Gymnodinium_spp	Species abundance	cells per liter (cells/L)	abundance
Gyrodinium_spp	Species abundance	cells per liter (cells/L)	abundance
Heterocapsa_rotundata	Species abundance	cells per liter (cells/L)	abundance
Heterocapsa_triquetra	Species abundance	cells per liter (cells/L)	abundance
Leptocylindrus_danicus	Species abundance	cells per liter (cells/L)	abundance
Leptocylindrus_minimus	Species abundance	cells per liter (cells/L)	abundance
Licmophora_spp	Species abundance	cells per liter (cells/L)	abundance
Melosira_spp	Species abundance	cells per liter (cells/L)	abundance
Odontella_spp	Species abundance	cells per liter (cells/L)	abundance
Paralia_sulcata	Species abundance	cells per liter (cells/L)	abundance
Pennate_unknown	Species abundance	cells per liter (cells/L)	abundance
Phaeocystis_spp	Species abundance	cells per liter (cells/L)	abundance
Pleurosigma_spp	Species abundance	cells per liter (cells/L)	abundance
Prorocentrum_spp	Species abundance	cells per liter (cells/L)	abundance
Protoperidinium_spp	Species abundance	cells per liter (cells/L)	abundance
Pseudonitzschia_spp	Species abundance	cells per liter (cells/L)	abundance
Rhabdonema_adriaticum	Species abundance	cells per liter (cells/L)	abundance
Rhizosolenia_pungens	Species abundance	cells per liter (cells/L)	abundance
Rhizosolenia_setigera	Species abundance	cells per liter (cells/L)	abundance
Rhizosolenia_spp	Species abundance	cells per liter (cells/L)	abundance
Rhizosolenia_styliformis_imbricata	Species abundance	cells per liter (cells/L)	abundance
Skeletonema_spp	Species abundance	cells per liter (cells/L)	abundance
Thalassionema_nitzschioides	Species abundance	cells per liter (cells/L)	abundance
Thalassiosira_nordenskioeldii	Species abundance	cells per liter (cells/L)	abundance
Thalassiosira_rotula	Species abundance	cells per liter (cells/L)	abundance
Thalassiosira_spp	Species abundance	cells per liter (cells/L)	abundance

Status message

Database

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Dataset: March Incubation Microscopy Cell Counts