Dataset: Average polyp survival – Fall 2018 experiment

Name: Survivorship of newly settled polyps of Antillogorgia bipinnata inoculated with one of five genotypes of Breviolum antillogorgium and reared at 26 and 30 degrees Celsius.
Published: 2021-11-16
Keywords: Symbiosis, Symbiodiniaceae, Octocorals, thermal tolerance, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.855116.1

Spatial Extent: N:24.7525 E:-81.754583 S:24.7525 W:-81.754583

Project:

RUI: Collaborative Research: Genetic variation as a driver of host and symbiont response to increased temperature on coral reefs (Host Symbiont Temp Response)

Principal Investigator:

Casey terHorst (California State University Northridge, CSUN)

Co-Principal Investigator:

Mary Alice Coffroth (State University of New York at Buffalo, SUNY Buffalo)

Contact:

Mary Alice Coffroth (State University of New York at Buffalo, SUNY Buffalo)

BCO-DMO Data Manager:

Karen Soenen (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2021-11-16

Restricted:

Release Date:

2021-10-01

Validated:

Yes

Current State:

Final no updates expected

Survivorship of newly settled polyps of Antillogorgia bipinnata inoculated with one of five genotypes of Breviolum antillogorgium and reared at 26 and 30 degrees Celsius.

Abstract:

Survivorship of newly settled polyps of Antillogorgia bipinnata inoculated with one of five genotypes of Breviolum antillogorgium and reared at 26 and 30 degrees Celsius.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2021-11-16	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.855116.1	2021-11-16

Methods & Sampling

Larvae were collected from Antillogorgia bipinnata colonies that were maintained in seawater tables at the Keys Marine Lab on Long Key, FL. Antillogorgia bipinnata colonies were initially collected from Tennessee Reef (N 24° 45.150' W 81° 45.275').

Breviolum antillogorgium cultures, representing five genotypes, were used to infect polyps reared from larvae collected from A. bipinnata. Three cultures had been grown at 26-degree C since isolation in 2016 (G1 [16-0590F], G2 [16-0875] and G3 [16-1631]) and two had been grown at 30-degree C since isolation in 2016 (G4 [16-0587] and G5 [16-0763]). Polyps from all treatments were reared at both 26 and 30 degree C in the lab under 12:12 light dark cycles. Containers with polyps were inoculated on Day 1 and Day 5. Starting on Day 32, containers were inoculated three times a week with water changes. Polyp locations in the containers were mapped and survival monitored every 3-4 days.

Data Processing Description

Survivorship was determined based on the number of polyps present within a given container divided by the initial number of polyps within that container.

Presence/absence of the polyps was assessed visual using a Leica dissecting microscope.

No BCO-DMO processing notes.

Data Files

File
polyp_survival.csv (Comma Separated Values (.csv), 25.63 KB) MD5:64114e4911fbba2f085c6c03c5d70d22 Primary data file for dataset ID 855116

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1559286

Deployments

None available yet

Instruments

Microscope - Optical

Supplied Name: Leica dissecting microscope

Supplied Description:

Status of the polyps (healthy or not healthy, expanded or not, dead) was assessed visually using a Leica dissecting microscope approximately every 3 days.

Instrument Type

Generic Name: Microscope - Optical

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/

Generic Description:

Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Temperature	Temperature at which the polyp was reared	Degrees Celsius (°C)	temperature
Day	Day indicates the timepoint, post-inoculation, at which survivorship was determined.	Unitless	sample_descrip
Container	Identification of container replicate	Unitless	sample_descrip
Survivorship	Proportion of polyps that were alive at that timepoint	Unitless	sample_descrip
Initial_number	Indicates the number of polyps that were in the container on Day 1	Unitless	sample_descrip
Genotype	Genotype of culture (based on 5 microsatellite loci) used to inoculate the polyps,	Unitless	sample_descrip

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Database

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Dataset: Average polyp survival – Fall 2018 experiment