Dataset: Cell counts in polyps – Fall 2018 experiment

Name: Cell counts in newly settled polyps of Antillogorgia bipinnata inoculated with one of six genotypes of Breviolum antillogorgium and reared at 26 and 30 degrees Celsius
Published: 2021-11-16
Keywords: Symbiosis, Symbiodiniaceae, Octocorals, thermal tolerance, biota, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.809388.1

Spatial Extent: N:24.7525 E:-81.754583 S:24.7525 W:-81.754583

Temporal Extent: 2018 - 2018

Project:

RUI: Collaborative Research: Genetic variation as a driver of host and symbiont response to increased temperature on coral reefs (Host Symbiont Temp Response)

Principal Investigator:

Casey terHorst (California State University Northridge, CSUN)

Co-Principal Investigator:

Mary Alice Coffroth (State University of New York at Buffalo, SUNY Buffalo)

Contact:

Mary Alice Coffroth (State University of New York at Buffalo, SUNY Buffalo)

BCO-DMO Data Manager:

Karen Soenen (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2021-11-16

Restricted:

Validated:

Current State:

Final no updates expected

Cell counts in newly settled polyps of Antillogorgia bipinnata inoculated with one of six genotypes of Breviolum antillogorgium and reared at 26 and 30 degrees Celsius

Abstract:

Cell counts in newly settled polyps of Antillogorgia bipinnata inoculated with one of six genotypes of Breviolum antillogorgium and reared at 26 and 30 degrees Celsius.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2021-11-16	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.809388.1	2021-11-16

Description

Cell counts in newly settled polyps of Antillogorgia bipinnata inoculated with one of six genotypes of Breviolum antillogorgium and reared at 26 and 30 degrees C.

Methods & Sampling

Larvae were collected from Antillogorgia bipinnata colonies that were maintained in seawater tables at the Keys Marine Lab on Long Key, FL. Antillogorgia bipinnata colonies were initially collected from Tennessee Reef (N 24° 45.150' W 81° 45.275')

Breviolum antillogorgium cultures, representing five genotypes, were used to infect polyps reared from larvae collected from A. bipinnata. Three cultures had been grown at 26-degree C since isolation in 2016 (G1 [16-0590F], G2 [16-0875] and G3 [16-1631]) and two had been grown at 30-degree C since isolation in 2016 (G4 [16-0587] and G5 [16-0763]). Polyps from all treatments were reared at both 26 and 30 degree C in the lab under 12:12 light dark cycles. When the experiment was terminated (69 days after initial inoculations), polyps were sampled to determine cell density.

Data Processing Description

Polyps (aged 92-115 days) were removed from incubation containers and homogenized in 0.1 ml filtered seawater. Two replicate cell counts of each polyp was made using light microscopy and a hemocytometer. Mean symbiont density per ml was calculated.

Mean x 10,000 were calculated from the counts to give cells/ml and then divided by 10 to give cells/polyp

BCO-DMO Processing notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions

Data Files

File
polyp_cell_count.csv (Comma Separated Values (.csv), 10.36 KB) MD5:ccefae1e38186b5f6c2fccda1bfea3ef Primary data file for dataset ID 809388

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1559286

Deployments

None available yet

Instruments

Hemocytometer

Supplied Name: Reichert Brightline hemocytometer

Supplied Description:

Used to make cell counts.

Instrument Type

Generic Name: Hemocytometer

Generic Description:

A hemocytometer is a small glass chamber, resembling a thick microscope slide, used for determining the number of cells per unit volume of a suspension. Originally used for performing blood cell counts, a hemocytometer can be used to count a variety of cell types in the laboratory. Also spelled as "haemocytometer". Description from:
http://hlsweb.dmu.ac.uk/ahs/elearning/RITA/Haem1/Haem1.html.

Microscope - Optical

Supplied Name: Zeiss

Supplied Description:

Instrument Type

Generic Name: Microscope - Optical

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/

Generic Description:

Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Container	Identification of container replicate	unitless	sample_descrip
Polyp_ID	Identification of polyp that was sampled given as container and mapped polyp number (eg. 2.06 would be container 2; mapped polyp 6)	unitless	sample
Culture	Culture used to inoculate the polyps	unitless	sample_descrip
Polyp_age	Age of the polyp	days	age
Temperature	Temperature at which the polyp was reared	Degrees C	temp
Count_1	First replicate of cells counted	unitless	count
Count_2	Second replicate of cells counted	unitless	count
Mean	Mean of replicate counts	unitless	count
Cells_ml	Cells in one ml	Cells ml-1	cell_concentration
Cells_polyp	Cells in the sampled polyp	Cells_polyp	cell_concentration

Status message

Database

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Dataset: Cell counts in polyps – Fall 2018 experiment