Dataset: Respirometry data for pelagic crustaceans, cephalopods, and fish collected on R/V Sikuliaq cruise SKQ201701S from January to February 2017

Final no updates expectedDOI: 10.26008/1912/bco-dmo.855732.1Version 1 (2021-07-22)Dataset Type:Cruise Results

Principal Investigator, Contact: Brad Seibel (University of South Florida)

Co-Principal Investigator: Christopher Neil Roman (University of Rhode Island)

Co-Principal Investigator: Karen Wishner (University of Rhode Island)

BCO-DMO Data Manager: Shannon Rauch (Woods Hole Oceanographic Institution)


Project: Collaborative Research: A metabolic index to predict the consequences of climate change for midwater ecosystems (Metabolic Index)


Abstract

This dataset comprises results of respirometry experiments conducted at sea using pelagic crustaceans, cephalopods, and fish collected on R/V Sikuliaq cruise SKQ201701S from January to February 2017. Experimental animals were collected using either a modified tucker trawl or MOCNESS.

This dataset comprises results of respirometry experiments conducted at sea. Experimental animals were collected using either a modified tucker trawl or MOCNESS. Metabolic rates and critical oxygen pressures were measured using published methods (Birk et al., 2018; 2019; Wishner et al., 2018; Seibel et al., 2021). Briefly, individual specimens were placed in darkened sealed chambers filled with 0.2 μm filtered seawater that had been treated with antibiotics (25 mg/L each of streptomycin and actinomycin) to minimize bacterial respiration. The ratio of chamber size to body mass was approximately 1:20. Oxygen content was continuously monitored using Pyroscience Firesting or Presens oxygen meters and fiber optic probes with oxygen-sensitive fluorescent spots. Oxygen meters were calibrated with air-saturated seawater and concentrated Na2SO3 solution (PO2 = 0). Chamber water was mixed with magnetic stirrers (Cole-Parmer Immersible Stirrer EW-04636-50) to ensure homogenous oxygen levels throughout the chamber. Oxygen consumption rates (MO2) were calculated from the slope of PO2 over time. Only linear regressions with an R2 > 0.8 were included. The median MO2 value from non-hypoxic oxygen levels was calculated for each individual. The Pcrit for each individual was calculated using three metrics, including the traditional "breakpoint" method, the "sub-prediction interval (sub-PI)" and the alpha-method.


Related Datasets

IsRelatedTo

Dataset: Copepod Abundances in MOCNESS tows
Wishner, K., Outram, D., Seibel, B., Roman, C. (2021) Abundances of copepod species in each net from MOCNESS tows in the Eastern Tropical North Pacific collected on four research cruises from 2007-2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-07-09 doi:10.26008/1912/bco-dmo.855395.1
IsRelatedTo

Dataset: SKQ201701S Event Log
Relationship Description: The SKQ201701S Event Log contains the date, time, latitude, and longitude of each event conducted during the cruise.
Wishner, K., Roman, C., Seibel, B. (2021) Event log from R/V Sikuliaq SKQ201701S from January to February 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-01-10 doi:10.26008/1912/bco-dmo.755088.1

Related Publications

Results

Birk, M. A., Mislan, K. A. S., Wishner, K. F., & Seibel, B. A. (2019). Metabolic adaptations of the pelagic octopod Japetella diaphana to oxygen minimum zones. Deep Sea Research Part I: Oceanographic Research Papers, 148, 123–131. doi:10.1016/j.dsr.2019.04.017
Results

Seibel, B. A., Andres, A., Birk, M. A., Burns, A. L., Shaw, C. T., Timpe, A. W., & Welsh, C. J. (2021). Oxygen supply capacity breathes new life into critical oxygen partial pressure (Pcrit). Journal of Experimental Biology, 224(8). doi:10.1242/jeb.242210
Results

Wishner, K. F., Seibel, B. A., Roman, C., Deutsch, C., Outram, D., Shaw, C. T., … Riley, S. (2018). Ocean deoxygenation and zooplankton: Very small oxygen differences matter. Science Advances, 4(12), eaau5180. doi:10.1126/sciadv.aau5180
Results

Wishner, K. F., Seibel, B., & Outram, D. (2020). Ocean deoxygenation and copepods: coping with oxygen minimum zone variability. Biogeosciences, 17(8), 2315–2339. doi:10.5194/bg-17-2315-2020
Methods

Birk, M. A., McLean, E. L., & Seibel, B. A. (2018). Ocean acidification does not limit squid metabolism via blood oxygen supply. Journal of Experimental Biology. doi:10.1242/jeb.187443