Dataset: Coral physiological measurements

Name: Montipora capitata and Porites compressa Physiological Measurements for Experimental Corals and Parent Colonies from 2014-2015 (RAPID Hawaii project)
Published: 2023-11-06
Keywords: coral physiology, bleaching, ocean acidification, feeding, Montipora capitata, Porites compressa, oceans

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Spatial Extent: N:21.435 E:-57.6817 S:21.3267 W:-57.7867

Temporal Extent: 2014-11 - 2015-12

Project:

Will corals recover from bleaching under ocean acidification conditions? (RAPID Hawaii)

Principal Investigator:

Andréa G. Grottoli (Ohio State University)

Robert J. Toonen (University of Hawaii)

Co-Principal Investigator:

Kerri Dobson (Ohio State University)

Dr Christopher P. Jury (University of Hawaii)

Contact:

Kerri Dobson (University of Southampton)

BCO-DMO Data Manager:

Sawyer Newman (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2023-11-06

Restricted:

Yes

Release Date:

2023-11-14

Validated:

Current State:

Preliminary and in progress

Montipora capitata and Porites compressa Physiological Measurements for Experimental Corals and Parent Colonies from 2014-2015 (RAPID Hawaii project)

Abstract:

Physiological data for Montipora capitata and Porites compressa: endosymbiont cell density (cells cm-2), Contribution of Total Carbon to Animal Respiration (CTAR, %), calcification (mg day-1 cm-2), biomass (g cm-2), total lipids (J gdw-1), photosynthesis and respiration (µmol hr-1 cm-2), Contribution of Zooxanthellae (Symbiodiniaceae) to Animal Respiration (CZAR, %), feeding rate (brine shrimp hr-1 cm-2), and Contribution of Heterotrophy to Animal Respiration (CHAR, %). Measurements for experimental corals were taken from one of three time points (November 2015, June 2014, or December 2015).

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Methods & Sampling

Between 10–12 November 2014, three weeks following the peak of the 2014 thermal stress event, six visibly non-bleached and six visibly bleached parent colonies of M. capitata and P. compressa were collected from 1–3m depth from the fringing reefs surrounding Moku o Loʻe in Kāneʻohe Bay (centered at 21°26’6” N, 157°47’12” W) and in Waimānalo Bay (centered at 21°19’36” N, 157°40’54” W) using a hammer and chisel. One ramet from each colony was immediately placed in a -20 °C freezer to evaluate the initial physiology of the parent colonies prior to experimental manipulation. An additional twelve ramets were collected from each parent colony of each species from each location (totaling 576 fragments) and mounted on pre-labeled plastic tiles for use in the manipulative experiment.

All experimental ramets were returned to the Hawai‘i Institute of Marine Biology and allowed to recover under ambient seawater conditions in outdoor, unfiltered, flow-through seawater tanks for three days. Natural light levels were reduced using 40% neutral density shade cloth such that photosynthetically active radiation levels mimicked that at collection depth.

On 15 November 2014, ramets were equally divided among eight outdoor, 38 L experimental tanks supplied with unfiltered, flow-through seawater. Though the incoming seawater was unfiltered, virtually no zooplankton is delivered to experimental tanks at HIMB due to scouring of the inflowing seawater by in-line invertebrates. Two experimental tanks were randomly assigned to each of the following four treatments: 1) Control (ambient seawater of pH 7.97 and unfed), 2) Fed (ambient seawater of pH 7.97 and fed), 3) OA (acidified seawater of pH 7.74 and unfed), and 4) OA+Fed (acidified seawater pH 7.74 and fed).

Coral holobiont physiology was assessed for parent colonies (i.e., in situ reef controls) and a subset of experimental corals in December 2014 (2 months after 2014 peak thermal stress; 1 month in experimental tanks), June 2015 (8 months after 2014 peak thermal stress; 7 months in experimental tanks), and November 2015 (one month after 2015 peak thermal stress; 13 months in experimental tanks).

Data Processing Description

Differences in the physiological profiles (including endosymbiont cell density, CTAR, calcification, tissue biomass, and total lipids) between species, sites, and sites within species was assessed with a multivariate analysis of similarity (ANOSIM).

Physiological profiles of experimental ramets in December 2014 and June 2015 were evaluated using both multivariate and univariate analyses.

Following the 2015 thermal stress event, almost all P. compressa ramets died, resulting in sample sizes too small for analysis in December 2015.

Additional details of statistical analyses are outlined in Dobson et al..

BCO-DMO Processing

Time_point (date) field converted from %d-%y (e.g., Nov-14) to %m-%Y (e.g., 11-2014) format

Added decimal degree representations of latitude and longitude to the dataset and rounded these fields to six degrees of precision

Empty cells represent "No data" within the dataset

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1514859
NSF Division of Ocean Sciences	OCE-1514861

Deployments

None available yet

Instruments

Dissolved Oxygen Sensor

Supplied Name: YSI ProODO Optical DIssolved Oxygen

Supplied Description:

YSI ProODO Optical Dissolved Oxygen probes were used to measure dissolved oxygen during coral incubations for use in the calculation of photosynthesis and respiration rates.

Instrument Type

Generic Name: Dissolved Oxygen Sensor

Generic Description:

An electronic device that measures the proportion of oxygen (O2) in the gas or liquid being analyzed

Spectrophotometer

Supplied Name: Thermo Scientific Genesys

Supplied Description:

A Thermo Scientific Genesys spectrophotometer was used to measure the intensity of light transmission at specific wavelengths for chlorophyll a.

Instrument Type

Generic Name: Spectrophotometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/

Generic Description:

An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Species	Species of parent colony from which samples were collected. MC = Montipora capitata; PC = Porites compressa.	unitless	species
Collection_site	Collection site representing the location of the parent colony from which samples were collected. KB = K_ne_ohe Bay; W = Waim_nalo Bay.	unitless	site
Latitude_decimal_degrees	Collection site latitude in decimal degrees. A positive value indicates a Northern coordinate.	decimal degrees	lat
Longitude_decimal_degrees	Collection site longitude in decimal degrees. A negative value indicates a Western coordinate.	decimal degrees	lon
Lat	Collection site latitude in degrees, minutes, seconds.	decimal degrees	lat
Long	Collection site longitude in degrees, minutes, seconds.	decimal degrees	lon
Colony	Colony identification number.	unitless	sample_descrip
Sample_ID	Unique sample identification number.	unitless	sample
Health_status	Health status of parent colony. NB = non-bleached; B = bleached.	unitless	sample_descrip
pH	Seawater pH of experimental tanks. AMB = ambient seawater pH (7.97); OA = acidified seawater / low pH (7.74).	unitless	treatment
Feeding_status	Feeding status of experimental tank. NF = not fed; F = fed.	unitless	treatment
Treatment	Treatment or parent colony status of experimental sample. Parent colony = parent colony; BL AMB F = bleached, ambient pH, fed; BL AMB NF = bleached, ambient pH, not fed; BL OA F = bleached, low pH, fed; BL OA NF = bleached, low pH, not fed; NB AMB F = non-bleached	unitless	treatment
Tank	Tank identification number.	unitless	tank
Time_point	Collection time for Parent colony and time of removal from experiment for those in treatment. Format: %m-%Y	unitless	time_point
Endo_density	Endosymbiont cell density of coral sample.	cells cm-2	density
Calc	Calcification of coral sample.	mg day-1 cm-2	calcification
Total_biomass	Total biomass of sample.	g cm-2	biomass
Total_lipids	Total lipids.	J gdw-1	lipids_tot
Gross_P	Gross photosynthesis.	umol O2 min-1 cm-2	photosynthesis
LEDR	Light enhanced dark respiration.	unitless	resp
CZAR	Contribution (percentage) of Zooxanthellae (Symbiodiniaceae) to Animal Respiration.	unitless	resp
FR	Feeding rate.	Brine shrimp hr-1 cm-2	food_consumption
CHAR	Contribution (percentage) of heterotrophy to animal respiration.	unitless	resp
CTAR	Contribution (percentage) of total fixed carbon to animal respiration.	unitless	resp

Database

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Dataset: Coral physiological measurements