Dataset: PNPC and Nitrogen Uptake Rates

Name: Particulate Carbon and Nitrogen data from CTD casts from R/V Hugh R. Sharp HRS1610 in the Mid-Atlantic coastal waters from August 2016 (CyanateInTheSea project)
Published: 2024-02-19
Keywords: nitrogen, uptakes, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.920423.1

Spatial Extent: N:37.6683 E:-73.3708 S:33.3306 W:-78.5848

Mid-Atlantic coastal waters, 33-38⁰ N, 73-79⁰ W

Temporal Extent: 2016-08-07 - 2016-08-17

Project:

Cyanate in the Sea: Sources, Sinks, and Quantitative Significance (CyanateInTheSea)

Chief Scientist:

Margaret Mulholland (Old Dominion University, ODU)

Principal Investigator:

Margaret Mulholland (Old Dominion University, ODU)

Student:

Yifan Zhu (Old Dominion University, ODU)

Contact:

Peter W. Bernhardt (Old Dominion University, ODU)

Margaret Mulholland (Old Dominion University, ODU)

Technician:

Peter W. Bernhardt (Old Dominion University, ODU)

Data Manager:

Peter W. Bernhardt (Old Dominion University, ODU)

Yifan Zhu (Old Dominion University, ODU)

BCO-DMO Data Manager:

Sawyer Newman (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2024-02-19

Restricted:

Validated:

Current State:

Preliminary and in progress

Particulate Carbon and Nitrogen data from CTD casts from R/V Hugh R. Sharp HRS1610 in the Mid-Atlantic coastal waters from August 2016 (CyanateInTheSea project)

Abstract:

Particulate Carbon and Nitrogen (PNPC) data collected from CTD casts aboard the R/V Huge R. Sharp cruise to Mid-Atlantic coastal waters in August 2016. Nitrogen uptake rates were calculated from 15N-isotope labeled incubation. The parameters include PN, PC, and six N species uptake rates by whole water community at three depths: near surface, the depth above the Chl maximum (or near the bottom of the mixed layer), and the depth of the Chl maximum.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2024-02-19	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.920423.1	2024-03-19

Methods & Sampling

Particulate Carbon and Nitrogen (PNPC)

Whole water from three target depths (near surface, the depth above the Chl maximum (or near the bottom of the mixed layer), and the depth of the Chl maximum) at each station was collected from Niskin bottles into 10 L carboys and transported to the on-board laboratory where water samples were mixed and sub-samples (0.2–1.4 L) were collected onto pre-combusted GF-75 filters (Whatman®, nominal pore size 0.3 μm) in triplicate, for analysis of particulate nitrogen (PN) and carbon (PC) concentrations and the natural abundance of 15N and 13C. The filters were stored in cryovials and immediately frozen and stored in a freezer at -20 °C until analysis. Prior to their analysis, filters were dried at 40 ºC, pelletized in tin capsules, and analyzed on a Europa 20/20 isotope ratio mass spectrometer equipped with an automated N and C analyzer. The detection limit of the mass spectrometer was 0.0018 and 0.0005 atom% for 15N and 13C, respectively; these values were derived based on three times the standard deviation (3 × SD) of the atom% of 12.5 µg N and 100 µg C standards (n=40).

Nitrogen Uptake

Parallel sets of triplicate whole water (0.5−2 L) from the three target depths mentioned above (near surface, above the depth of the Chl maximum (or near the bottom of the mixed layer), and at the depth of the Chl maximum) from 27 stations were dispensed from 10 L carboys into acid-cleaned polyethylene terephthalate glycol incubation bottles (NalgeneTM).

Nitrogen uptake incubations were initiated by amending incubation bottles with highly enriched (98−99%) 15N-labeled substrates (Cambridge Isotope Laboratories, Inc., USA), including ammonium chloride (15NH4Cl), potassium nitrate (K15NO3), potassium nitrite (K15NO2), and 15N- and 13C-labeled potassium cyanate (KO13C15N), urea (13CO(15NH2)2), and algal amino acid mixture.

Problems/Issues

Nitrogen uptake rates should be considered as the potential rates rather than in-situ rates.

Data Processing Description

Nitrogen update rates were calculated via the formula included in the supplemental files section of this metadata landing page.

Within this formula, (atom% PN)initial and (atom% PN)final represent the 15N isotopic composition of the particulate pool at the initial and final time points of the incubation period; atom% N source pool is the 15N isotopic enrichment of the dissolved nitrogen pool after the tracer addition; [PN] represents the concentration of the particulate nitrogen pool; here, we used the average value between the initial and final PN concentrations for this calculation.

BCO-DMO Processing

- Converted date values from %m/%d/%Y format to %Y-%m-%d format

Data Files

File
920423_v1_PNPC_and_Nitrogen_Uptake_Rates.csv (Comma Separated Values (.csv), 26.01 KB) MD5:9e973dc5c28f062e90bc12b79e068fc7 Primary data file for dataset ID 920423, version 1

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1459698

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
HRS1610	Cyanate In the Sea	2016-08-05	R/V Hugh R. Sharp	Margaret Mulholland (Chief Scientist)	data info

Instruments

Mass Spectrometer

Supplied Name:

Supplied Description:

An isotope ratio mass spectrometer was used for PNPC measurement.

Instrument Type

Generic Name: Mass Spectrometer

Acronym: Mass Spec

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/

Generic Description:

General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components.

Niskin bottle

Supplied Name: 10 L Niskin bottles

Supplied Description:

Dissolved seawater samples were collected using a sampling rosette equipped with a 12 X 10 L Niskin bottles.

Instrument Type

Generic Name: Niskin bottle

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/

Generic Description:

A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Station	Station number (1-41)	unitless	sta_id
Cast	CTD cast number	unitless	cast
ISO_DateTime_UTC	Datetime of CTD cast in UTC Format: %Y-%m-%dT%H:%M:%SZ	unitless	ISO_DateTime_UTC
Latitude	Latitude of cast location in decimal degrees; a positive value indicates a Northern coordinate	decimal degrees	lat
Longitude	Longitude of cast location in decimal degrees; a negative value indicates a Western coordinate	decimal degrees	lon
Depth	Depth value from CTD	meters (m)	depth
Temp	Temperature value from CTD	Celcius (C)	temp
Salinity	Salinity value from CTD	practical salinity unit (PSU)	sal
Fluorescence	Fluorescence value from WET Labs ECO-AFL/FL	milligram per cubic meter (mg/m^3)	fluor
Total_Chl	Total chlorophyll (chl) value measured through high-performance liquid chromatoraphy (HPLC)	microgram per liter (µg/L^1)	chl_a
Chl_SD	Standard deciation of chlorophyll	microgram per liter (µg/L^1)	std_dev
Ammonium	Ambient NH4 concentration	micromole per liter (µmol/L^1)	NH4
Nitrate	Ambient NO3 concentration	micromole per liter (µmol/L^1)	NO3
Nitrite	Ambient NO2 concentration	micromole per liter (µmol/L^1)	NO2
Urea	Ambient Urea concentration	micromole N per liter (µmol N /L^1)	Urea
Phosphate	Ambient PO4 concentration	micromole per liter (µmol/L^1)	PO43-
Dissolved_free_amino_acids	Ambient Dissloved free amino acids concentration	micromole N per liter (µmol N /L^1)	amino_conc
Cyanate	Ambient cyanate concentration	micromole per liter (µmol/L^1)	cyanate
PC	Initial particulate carbon concentration	micromole per liter (µmol/L^1)	TPC
PN	Initial particulate nitrogen concentration	micromols N per liter (µmol N/L^1)	N
NH4_avg_uptake_rate	Ammonium average uptake rate	micromols N per liter per hour (µmol N /L^1/h^1)	NH4
SD_NH4	Standard deviation of ammonium uptake rate (triplicates)	micromols N per liter per hour (µmol N /L^1/h^1)	std_dev
NO3_avg_uptake_rate	Nitrate average uptake rate	micromols N per liter per hour (µmol N /L^1/h^1)	NO2
SD_NO3	Standard deviation of nitrate uptake rate (triplicates)	micromols N per liter per hour (µmol N /L^1/h^1)	std_dev
NO2_avg_uptake_rate	Nitrite average uptake rate	micromols N per liter per hour (µmol N /L^1/h^1)	NO2
SD_NO2	Standard deviation of nitrite uptake rate (triplicates)	micromols N per liter per hour (µmol N /L^1/h^1)	std_dev
Urea_avg_uptake_rate	Urea average uptake rate	micromols N per liter per hour (µmol N /L^1/h^1)	Urea
SD_Urea	Standard deviation of urea uptake rate (triplicates)	micromols N per liter per hour (µmol N /L^1/h^1)	std_dev
AAs_avg_uptake_rate	Amino acids average uptake rate	micromols N per liter per hour (µmol N /L^1/h^1)	pAmino
SD_AAs	Standard deviation of amino acids uptake rate (triplicates)	micromols N per liter per hour (µmol N /L^1/h^1)	std_dev
Cyanate_avg_uptake_rate	Cyanate average uptake rate	micromols N per liter per hour (µmol N /L^1/h^1)	pCyanate
SD_cyanate	Standard deviation of cyanate uptake rate (triplicates)	micromols N per liter per hour (µmol N /L^1/h^1)	std_dev
Specific_NH4_uptake_rate	Specific ammonium value independent from partiulate nitrogen concentration	per hour (h^-1)	pNH4
Specific_NO3_uptake_rate	Specific nitrate uptake rate value independent from particulate nitrogen concentration	per hour (h^-1)	pNO3
Specific_NO2_uptake_rate	Specific nitrite value independent from particulate nitrogen concentration	per hour (h^-1)	NO2
Specific_urea_uptake_rate	Specific urea uptake rate value independent from particulate nitrogen concentration	per hour (h^-1)	pUrea
Specific_AAs_uptake_rate	Specific amino acid uptake rate independent from particulate nitrogen concentration	per hour (h^-1)	pAmino
Specific_cyanate_uptake_rate	Specific cyanate uptake rate independent from particulate nitrogen contentration	per hour (h^-1)	cyanate

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Dataset: PNPC and Nitrogen Uptake Rates