Dataset: Kaneohe Bay microzooplankton growth and mortality

Name: Metadata for field dilution experiments to measure community microzooplankton grazing rates in Kaneohe Bay, HI from 2012-2013 (EAGER: Copepod nauplii project)
Published: 2016-02-04
Keywords: biota, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.637670.1

Spatial Extent: N:21.4322 E:-157.7797 S:21.4322 W:-157.7797

Temporal Extent: 2012-03-16 - 2013-06-05

Project:

EAGER: New molecular methods for studying copepod nauplii in the field (EAGER: Copepod nauplii)

Principal Investigator:

Erica Goetze (University of Hawaiʻi at Mānoa, SOEST)

Co-Principal Investigator:

Petra H. Lenz (University of Hawaiʻi at Mānoa, SOEST)

Karen E. Selph (University of Hawaiʻi at Mānoa, SOEST)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2016-02-04

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Metadata for field dilution experiments to measure community microzooplankton grazing rates in Kaneohe Bay, HI from 2012-2013 (EAGER: Copepod nauplii project)

Abstract:

Overview of community microzooplankton dilution experiments, and Chl-based growth and mortality rates. All experimental water taken from Kaneohe South Bay station and processed on shore within 2 hours of collection on the collection date. Samples for Chl a (fluorometric) filtered and frozen within 5 h of collection, after storage in dark bottles in a cooler. All rate data are adjusted for pigment photoadaptation during the incubation, using red fluorescence from parallel flow cytometry samples (see text for details). *March 2012 chlorophyll data are conservative estimates: most chlorophyll had degraded to phaeopigments when fluorometrically analyzed, so the values represent the sum of Chl a and phaeopigment. No rate data were calculated from these samples.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2016-02-04	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.637670.1	2021-01-19

Methods & Sampling

Phytoplankton Rates: µ0, µN, m (gross growth rate, nutrient-amended gross growth rate, and mortality rate, respectively): Phytoplankton growth and mortality rates were determined with seawater dilution experimental manipulations of freshly collected surface seawater as originally described in Landry & Hassett (1982), with modifications as described by Selph et al. (2005).

Data Processing Description

BCO-DMO Processing:

- added conventional header with dataset name, PI name, version date, reference information
- renamed parameters to BCO-DMO standard
- reformatted date from d-Mon-yy to yyyy-mm-dd
- replaced spaces with underscores
- added lat/lon columns

Data Files

File
growth_KB.csv (Comma Separated Values (.csv), 734 bytes) MD5:6a687ea6221c5e37a0a4f5f9b80c08ba Primary data file for dataset ID 637670

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1255697

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
Goetze_2012-2013		2012-03-16	lab UHawaii_SOEST	Erica Goetze (Principal Investigator)	data info

Instruments

Flow Cytometer

Supplied Name:

Supplied Description:

Instrument Type

Generic Name: Flow Cytometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/

Generic Description:

Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm)

Fluorometer

Supplied Name:

Supplied Description:

Instrument Type

Generic Name: Fluorometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/113/

Generic Description:

A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
KBG_expt	experiment id	unitless	exp_id
date_local	local date	yyyy-mm-dd	date_local
lat	latitude; north is positive	decimal degrees	lat
lon	longitude; east is positive	decimal degrees	lon
chla_init	initial chlorophyll-a concentration	micrograms/liter	chl_a
growth_init	gross growth rate	per day	growth
mortality	mortality rate	per day	no_bcodmo_term
r_squared	the coefficient of determination	unitless	no_bcodmo_term
growth_nuts	nutrient-amended gross growth rate	per day	growth
comment	comments	unitless	comment

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Database

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Dataset: Kaneohe Bay microzooplankton growth and mortality