Dataset: bacteria_water

DOI:10.1575/1912/bco-dmo.2350.1
Spatial Extent: N:-65.965 E:-67.049 S:-69.888 W:-72.427
Temporal Extent: 2001-04-29 - 2001-08-20
Project: 
Program: 
Principal Investigator: 
Chris H. Fritsen (Desert Research Institute, DRI)
BCO-DMO Data Manager: 
Ms Dicky Allison (Woods Hole Oceanographic Institution, WHOI BCO-DMO)
Version: 
1
Version Date: 
2002-11-15
Restricted: 
No
Validated: 
Yes
Current State: 
Final no updates expected
Data URL:
 https://www.bco-dmo.org/dataset/2350/data

Water column bacteria data from ARSV Laurence M. Gould cruises LMG0104 and LMG0106 in the Southern Ocean in 2001 (SOGLOBEC project; Sea Ice Microbes project)
Abstract: 
Water column bacteria data from ARSV Laurence M. Gould cruises LMG0104 and LMG0106 in the Southern Ocean in 2001 (SOGLOBEC project; Sea Ice Microbes project)

Expand/Collapse All
Description

Bacteria Abundance, Biomass and Chlorophyll: Water Column Samplesa

BG 235 - Methods used for chlorophyll a (chla) analysis and bacteria biomass determination

Core Sampling techniques:

Sampling methods for recovery of chlorophyll a and bacteria from sea ice cores follows those described in 
Garrison and Buck (1986)

Recommendations for reporting were used as outlined by: Horner, R. et al.,(1992)

Analytic Techniques:

Chla (mg m-3):

  • determined fluorometrically (Turner Designs 10AU Fluorometer) following extraction in 90% acetone (Parsons et al., 1984)
  • ice core chla corrected to account for chla in filtered sea water (FSW) added to core sections during melting

Bacteria cell abundance (cells m-3) and biomass (mg C m-3):

LMG 0106

  • preserved (0.5% glutaraldehyde) samples stained with 4',6-diamidino-2-phenylindole (DAPI; 0.1 to 0.3% final concentration), filtered through 0.2 mm black, polycarbonate membrane filters, and mounted onto glass microscope slides on the ship (within 24 hours following collection)
  • bacteria enumerated using epifluorescence microscopy and sized using digital images taken with Image Pro Plus
  • bacteria biomass determined using cell abundance, cell biovolume (BV; mm3; as determined from mean length and width measurements), and an allometric conversion factor for bacterial carbon per volume specific for DAPI-stained bacteria (cellular carbon = 218 X BV0.86; Loferer-Krossbacher et al., 1998).
  • ice core samples corrected for FSW dilution

NBP 0104

  • preserved (0.5% glutaraldehyde) samples stained with SYBR Gold (0.01% final concentration), filtered through 0.2 mm Anodisc filters (Whatman), and mounted onto glass microscope slides at home institution (~1-2 months following collection)
  • bacteria enumerated using epifluorescence microscopy and sized using digital images taken with Image Pro Plus
  • bacteria biomass determined using cell abundance, cell biovolume (BV; mm3), and an allometric conversion factor for bacterial carbon per volume specific for Acridine Orange-stained bacteria (cellular carbon = 89.9 X BV0.59; Simon and Azam, 1989). Note: an AO-specific carbon per volume conversion factor was used in calculating biomass in SYBR Gold-stained samples because both AO and SYBR Gold stain bacteria cells similarly relative to DAPI (unpublished data).
  • ice core samples corrected for FSW dilution

Data from LMG0106 (July-August, 2001) added in June 2002.

Updated: April 21, 2006

More Information about this dataset