Dataset: Coral iCA Activity

Name: Internal carbonic anhydrase activity in three species of coral collected from the Florida Keys in August 2013
Published: 2020-02-26
Keywords: oceans

Cite This Dataset

DOI:10.1575/1912/bco-dmo.794342.1

Spatial Extent: N:25.1193 E:-80.3008 S:25.1017 W:-80.4391

Temporal Extent: 2013-08 - 2013-08

Project:

Ocean Acidification: Coral Inorganic Carbon Processing in Response to Ocean Acidification (OA_coral_Ci_acquisition)

Program:

Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA) (SEES-OA)

Ocean Carbon and Biogeochemistry (OCB)

Principal Investigator:

Brian M. Hopkinson (University of Georgia, UGA)

BCO-DMO Data Manager:

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2020-02-26

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Internal carbonic anhydrase activity in three species of coral collected from the Florida Keys in August 2013

Abstract:

Internal carbonic anhydrase activity in three species of coral collected from the Florida Keys in August 2013.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2020-02-26	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.1575/1912/bco-dmo.794342.1	2020-03-04

Methods & Sampling

Fragments of Orbicella faveolata, Porites astreoides were collected from Little Grecian reef (25.1193 N 80.3008 W) and Siderastrea radians was collected from Florida Bay (25.1017 N, 80.4391 W) in Key Largo, FL, USA, in August of 2013 as permitted by the Florida Keys National Marine Sanctuary (FKNMS-2011-093, FKNMS-2014-015). Exposed skeleton was covered with modeling clay, and the colonies were maintained in closed circulation tanks filled with reef seawater, allowing at least 2 days of recovery after collection prior to experimentation. The tank was exposed to a natural light regime, with shading added at midday to keep solar irradiance below 600 umol photons m−2 s−1.

Data Processing Description

Coral external carbonic anhydrase (CA) activity was measured on each fragment using an 18O-exchange technique described in (Tansik et al. 2015). Coral tissue was then removed using an air-brush filled with artificial seawater. The coral-algal slurry was homogenized and then centrifuged to remove symbiotic algae. A portion of the purified homogenate was then assessed for total CA activity using an 18O-exchange approach. Internal CA activity was determined by subtracting external CA activity from total CA activity. All 18O-exchange data was processed using MATLAB code, which is publicly available at https://github.com/bmhopkinson/18O_processing. Methodological details are described in Hopkinson et al. (2015).

Note:
Brian Hopkinson's github repo has been forked by BCO-DMO to ensure a copy of the code is available. This can be accessed at https://github.com/BCODMO/18O_processing. A .zip file of the code is also attached to this dataset as a Supplemental File.

BCO-DMO Processing:
- modified columnn names;
- added columns for: Latitude, Longitude, Collection_Date;
- forked original github repo and downloaded a .zip file of the code.

Data Files

File
coral_iCA_activity.csv (Comma Separated Values (.csv), 1.21 KB) MD5:8f168bb3568005b41ff7520b22b806a1 Primary data file for dataset ID 794342

Supplemental Files

File
18O processing repo filename: 18O_processing-master.zip (ZIP Archive (ZIP), 67.46 KB) MD5:4b74426c332fba1e35acdc0d481fa6d9 MATLAB code for processing 18O-exchange data; associated with dataset 794342. PI: Brian Hopkinson. Also available from BCO-DMO github at https://github.com/BCODMO/18O_processing

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Emerging Frontiers Division	EF-1315944

Deployments

None available yet

Instruments

Mass Spectrometer

Supplied Name: Pfeiffer QMS220M2

Supplied Description:

18O-exchange was measured using a Pfeiffer QMS220M2 mass spectrometer.

Instrument Type

Generic Name: Mass Spectrometer

Acronym: Mass Spec

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/

Generic Description:

General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Species	Species name	unitless	species
Sample	Sample ID	unitless	sample
Longitude	Longitude of sample collection	decimal degrees	lon
Latitude	Latitude of sample collection	decimal degrees	lat
kif	Internal carbonic anhydrase (CA) activity	/s	no_bcodmo_term
Collection_Date	Year and month of sample collection; format: yyyy-mm	unitless	date

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Dataset: Coral iCA Activity

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Dataset: Coral iCA Activity

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