Dataset: Internal carbonic anhydrase activity in three species of coral collected from the Florida Keys in August 2013

ValidatedFinal no updates expectedDOI: 10.1575/1912/bco-dmo.794342.1Version 1 (2020-02-26)Dataset Type:Other Field Results

Principal Investigator: Brian M. Hopkinson (University of Georgia)

BCO-DMO Data Manager: Shannon Rauch (Woods Hole Oceanographic Institution)


Program: Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA) (SEES-OA)

Program: Ocean Carbon and Biogeochemistry (OCB)

Project: Ocean Acidification: Coral Inorganic Carbon Processing in Response to Ocean Acidification (OA_coral_Ci_acquisition)


Abstract

Internal carbonic anhydrase activity in three species of coral collected from the Florida Keys in August 2013.

Fragments of Orbicella faveolata, Porites astreoides were collected from Little Grecian reef (25.1193 N 80.3008 W) and Siderastrea radians was collected from Florida Bay (25.1017 N, 80.4391 W) in Key Largo, FL, USA, in August of 2013 as permitted by the Florida Keys National Marine Sanctuary (FKNMS-2011-093, FKNMS-2014-015). Exposed skeleton was covered with modeling clay, and the colonies were maintained in closed circulation tanks filled with reef seawater, allowing at least 2 days of recovery after collection prior to experimentation. The tank was exposed to a natural light regime, with shading added at midday to keep solar irradiance below 600 umol photons m−2 s−1.


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Results

Hopkinson, B. M., Tansik, A. L., & Fitt, W. K. (2015). Internal carbonic anhydrase activity in the tissue of scleractinian corals is sufficient to support proposed roles in photosynthesis and calcification. Journal of Experimental Biology, 218(13), 2039–2048. doi:10.1242/jeb.118182
Methods

Tansik, A. L., Fitt, W. K., & Hopkinson, B. M. (2015). External carbonic anhydrase in three Caribbean corals: quantification of activity and role in CO2 uptake. Coral Reefs, 34(3), 703–713. doi:10.1007/s00338-015-1289-8