Dataset: Gulf of Mexico in situ nitrate uptake

Name: Nitrate uptake from in-situ incubations conducted during R/V Nancy Foster cruises NF1704 and NF1802 in the Gulf of Mexico in May of 2017 and 2018
Published: 2021-01-14
Keywords: oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.836202.1

Spatial Extent: N:28.3358 E:-87.3032 S:25.7211 W:-90.1779

Temporal Extent: 2017-05-11 - 2018-05-18

Project:

Collaborative Research: Mesoscale variability in nitrogen sources and food-web dynamics supporting larval southern bluefin tuna in the eastern Indian Ocean (BLOOFINZ-IO)

Effects of Nitrogen Sources and Plankton Food-Web Dynamics on Habitat Quality for the Larvae of Atlantic Bluefin Tuna in the Gulf of Mexico (GoMex Tuna Foodweb B)

Program:

Second International Indian Ocean Expedition (IIOE-2)

Principal Investigator:

Michael Stukel (Florida State University, FSU)

Co-Principal Investigator:

Thomas Kelly (Florida State University, FSU)

Version:

Version Date:

2021-01-14

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Nitrate uptake from in-situ incubations conducted during R/V Nancy Foster cruises NF1704 and NF1802 in the Gulf of Mexico in May of 2017 and 2018

Abstract:

Nitrate uptake from in-situ incubations conducted during R/V Nancy Foster cruises NF1704 and NF1802 in the Gulf of Mexico in May of 2017 and 2018.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2021-01-14	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.836202.1	2021-04-06

Description

In addition to the funding sources listed in the "Funding Source" section, this dataset was partially funded by:

National Oceanic and Atmospheric Administration's RESTORE Program Grant (Project Title: Effects of nitrogen sources and plankton food-web dynamics on habitat quality for the larvae of Atlantic bluefin tuna in the Gulf of Mexico) under federal funding opportunity NOAA-NOS-NCCOS-2017-2004875, including NOAA JIMAR Cooperative Agreement, award #NA16NMF4320058, NOAA CIMAS Cooperative Agreement, award #NA15OAR4320064, and NOAA CIMEAS Cooperative Agreement, award #NA15OAR4320071.

Methods & Sampling

Nitrate uptake rates were measured at 6 depths spanning the euphotic zone (surface to deep chlorophyll maximum). Two incubation bottles (2.7 L) per depth were filled from Niskin rosettes and spiked with 15NO3- (final concentration of 10 or 8 nmol L-1 on NF17 and NF18, respectively). Bottles were then incubated for 24 h on an in situ incubation array (Landry et al. 2009). Upon recovery, incubations were immediately vacuum filtered onto pre-combusted 25-mm GF/F filters in the dark. Filters were rinsed with filtered seawater, wrapped in foil and stored at 80°C. On land, samples were fumigated with HCl vapor to remove inorganic carbon, dried, and placed inside a tin cup for C/N and isotopic analysis at the UC Davis stable isotope facility. Nitrate uptake was computed following recommendations in Stukel (2020). For additional details, see Yingling et al. (submitted).

Data Processing Description

In situ nitrate uptake rates were estimated using either equation ρkan,is (Eq. 6) or ρreg,is (Eq. 14) from Stukel (2020). ρkan,is was used if there was not evidence for high recycling within the incubation bottle (Φ<0.6). ρreg,is was used if there was evidence for high recycling within the incubation bottle (Φ>0.6). See Fig. 4 of Stukel (2020).

BCO-DMO Data Manager Processing Notes:
* Data in original excel file "In situ nitrate uptake.xlsx" exported as csv with the formatting that was set in Excel.
* modified parameter names to conform with BCO-DMO naming conventions: only A-Za-z0-9 and underscore allowed. Can not start with a number. (spaces, +, and - changed to underscores).
* Converted Date format to ISO 8601 format yyyy-mm-dd

Data Files

File
insitu_nitrate_uptake.csv (Comma Separated Values (.csv), 48.05 KB) MD5:2e02616604e14ae880349bb3b52b1d30 Primary data file for dataset ID 836202

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1851347
National Oceanic and Atmospheric Administration	NA16NMF4320058

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
NF1704	BLOOFINZ-GoM	2017-05-07	R/V Nancy Foster	Estrella Malca (Chief Scientist)	data info
NF1802	BLOOFINZ-GoM	2018-04-27	R/V Nancy Foster	John Lamkin (Chief Scientist)	data info

Instruments

CTD - profiler

Supplied Name:

Supplied Description:

Instrument Type

Generic Name: CTD - profiler

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/130/

Generic Description:

The Conductivity, Temperature, Depth (CTD) unit is an integrated instrument package designed to measure the conductivity, temperature, and pressure (depth) of the water column. The instrument is lowered via cable through the water column. It permits scientists to observe the physical properties in real-time via a conducting cable, which is typically connected to a CTD to a deck unit and computer on a ship. The CTD is often configured with additional optional sensors including fluorometers, transmissometers and/or radiometers. It is often combined with a Rosette of water sampling bottles (e.g. Niskin, GO-FLO) for collecting discrete water samples during the cast.

This term applies to profiling CTDs. For fixed CTDs, see https://www.bco-dmo.org/instrument/869934.

Elemental Analyzer

Supplied Name: PDZ Europa ANCA-GSL elemental analyzer

Supplied Description:

PDZ Europa ANCA-GSL elemental analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer (Sercon Ltd., Cheshire, UK). C/N and isotopic analysis at the UC Davis stable isotope facility.

Instrument Type

Generic Name: Elemental Analyzer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/

Generic Description:

Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material.

Isotope-ratio Mass Spectrometer

Supplied Name: PDZ Europa 20-20 isotope ratio mass spectrometer (Sercon Ltd., Cheshire, UK)

Supplied Description:

PDZ Europa ANCA-GSL elemental analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer (Sercon Ltd., Cheshire, UK). C/N and isotopic analysis at the UC Davis stable isotope facility.

Instrument Type

Generic Name: Isotope-ratio Mass Spectrometer

Acronym: IR Mass Spec; IRMS

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/

Generic Description:

The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer).

Niskin bottle

Supplied Name:

Supplied Description:

Instrument Type

Generic Name: Niskin bottle

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/

Generic Description:

A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Sigma_a	Estimated measurement uncertainty (sigma, σ) for "a" (Assumed ratio of regeneration to uptake).	dimensionless	no_bcodmo_term
T	Duration of Incubation in decimal hours.	hours	time_elapsed
Sigma_Ip_0	Estimated measurement uncertainty (sigma, σ) for Ip(0)	atom percent (%)	no_bcodmo_term
Sigma_I_amb	Estimated measurement uncertainty (sigma, σ) for I_amb	atom percent (%)	NH4
Sigma_I_spk	Estimated measurement uncertainty (sigma, σ) for I_spk	atom percent (%)	no_bcodmo_term
Sigma_L10Ks	Estimated measurement uncertainty (sigma, σ) for L10KS	micromoles of nitrogen per liter (µmol N L-1)	no_bcodmo_term
Sigma_N_amb	Estimated measurement uncertainty (sigma, σ) for [N]amb	micromoles of nitrogen per liter (µmol N L-1)	no_bcodmo_term
Sigma_N_spk	Estimated measurement uncertainty (sigma, σ) for [N]spk	micromoles of nitrogen per liter (µmol N L-1)	no_bcodmo_term
Sigma_T	Estimated measurement uncertainty (sigma, σ) for duration	hours	time_elapsed
Sigma_Ip_T	Estimated measurement uncertainty (sigma, σ) for Ip(T)	atom percent (%)	no_bcodmo_term
Sigma_P	Estimated measurement uncertainty (sigma, σ) for PON	micromoles of nitrogen per liter (µmol N L-1)	PON
I_amb	Isotope ratio of ambient ammonium	atom percent (%)	NH4
Sigma_Nitrate_Uptake	Propagated measurement uncertainty (sigma, σ) for nitrate uptake	nanomoles of nitorgen per liter per hour (nmol N L-1 h-1)	no_bcodmo_term
N_amb	Ambient nitrate concentration	micromoles of nitrogen per liter (µmol N L-1)	NO3
L10KS	Assumed log10(half-saturation constant)	micromoles of nitrogen per liter (µmol N L-1)	no_bcodmo_term
a	Assumed ratio of regeneration to uptake	dimensionless	no_bcodmo_term
Cast	CTD-Niskin rosette cast number	unitless	cast
Date	Date of collection and incubation Format: %Y-%m-%d	unitless	date_local
Depth	Depth	meters (m)	depth
N_spk	Final concentration of tracer spike. [N]spk	micromoles of nitrogen per liter (µmol N L-1)	no_bcodmo_term
Ip_0	Ip(0), Isotope ratio of PON (initial)	atom percent (%)	no_bcodmo_term
Ip_T	Ip(T), Isotope ratio of PON (final)	atom percent (%)	no_bcodmo_term
I_spk	Isotope ratio of tracer spike	atom percent (%)	no_bcodmo_term
Cycle	Lagrangian Experiment Number	unitless	exp_id
Lat	Latitude	decimal degrees	lat
Lon	Longitude	decimal degrees	lon
Cruise	Name of cruise	unitless	cruise_id
Nitrate_Uptake	Nitrate Uptake	nanomoles of nitorgen per liter per hour (nmol N L-1 h-1)	no_bcodmo_term
P	PON, Particulate organic nitrogen (final)	micromoles of nitrogen per liter (µmol N L-1)	PON
Sample	Sample name	unitless	sample

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Dataset: Gulf of Mexico in situ nitrate uptake