Dataset: Measured experimental tank parameters

Name: Experimental tank parameters throughout the life of Clathromorphum compactum and C. nereostratum calcification experiment from from 2015-2016 (CorallineAlgaePaleo-pH project)
Published: 2022-04-04
Keywords: oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.872498.1

Temporal Extent: 2015-10-08 - 2016-02-09

Project:

Collaborative Research: Development and application of a method using coralline algae to reconstruct past changes in pH and impacts on calcification (CorallineAlgaePaleo-pH)

Principal Investigator:

Justin B. Ries (Northeastern University)

Co-Principal Investigator:

Douglas B. Rasher (Bigelow Laboratory for Ocean Sciences)

Branwen Williams (University of California-San Diego, UCSD-SIO)

Scientist:

Isaac Westfield (Northeastern University)

Contact:

Justin B. Ries (Northeastern University)

BCO-DMO Data Manager:

Sawyer Newman (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2022-04-04

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Experimental tank parameters throughout the life of Clathromorphum compactum and C. nereostratum calcification experiment from from 2015-2016 (CorallineAlgaePaleo-pH project)

Abstract:

Regularly measured tank conditions (pH, Temperature, Salinity). measured three times per week throughout the life of Clathromorphum compactum and C. nereostratum calcification experiment (https://www.bco-dmo.org/dataset/871633). pCO2 was calculated every ~14 days from water samples.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2022-04-04	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.872498.1	2022-04-19

Methods & Sampling

Methodology:

Sampling and analytical procedures:

C. compactum and C. nereostratum cultured for 4 months under 12 pCO2/T treatments in thirty-six, 42-liter aquaria.

Seawater samples were obtained every ~14 days using 250 mL ground-glass-stoppered borosilicate glass bottles for measurement of total alkalinity (TA) and dissolved inorganic carbon (DIC) and other carbonate parameters.

Temp, salinity, pH measured three times weekly.

For buoyant weight, specimens were suspended beneath a balance in an aquarium at 4 cm depth in seawater of constant temperature and salinity.

Specimens were dosed with calcein before the experiment and the vertical growth was determined by measuring the vertical growth from the resulting calcein line to the surface.

Data Processing Description

CO2Sys used to calculate all carbonate parameters, including pCO2.

Data Files

File
Salinity_Temp_PCO2_tank_parameters.csv (Comma Separated Values (.csv), 97.79 KB) MD5:1c9da6167e9082edb27b2b9393c81089 Primary data file for dataset ID 872498

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1459706
NSF Division of Ocean Sciences	OCE-1459827
NSF Arctic Sciences	PLR-1316141

Deployments

None available yet

Instruments

MARIANDA VINDTA 3C total inorganic carbon and titration alkalinity analyser

Supplied Name: VINDTA 3C (Marianda Corporation, Kiel, Germany)

Supplied Description:

Measures total alkalinity and DIC using closed-cell potentiometric Gran titration and coulometry (UIC 5400), with both methods calibrated using certified Dickson DIC/TA standards.

Instrument Type

Generic Name: MARIANDA VINDTA 3C total inorganic carbon and titration alkalinity analyser

Acronym: inorganic carbon and alkalinity analyser

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0481/

Generic Description:

The Versatile INstrument for the Determination of Total inorganic carbon and titration Alkalinity (VINDTA) 3C is a laboratory alkalinity titration system combined with an extraction unit for coulometric titration, which simultaneously determines the alkalinity and dissolved inorganic carbon content of a sample. The sample transport is performed with peristaltic pumps and acid is added to the sample using a membrane pump. No pressurizing system is required and only one gas supply (nitrogen or dry and CO2-free air) is necessary. The system uses a Metrohm Titrino 719S, an ORION-Ross pH electrode and a Metrohm reference electrode. The burette, the pipette and the analysis cell have a water jacket around them. Precision is typically +/- 1 umol/kg for TA and/or DIC in open ocean water.

pH Sensor

Supplied Name: AccuFet™ Solid-State pH probe

Supplied Description:

AccuFet™ Solid-State pH probe, calibrated with 7.00 and 10.01 NBS buffers (for slope of calibration) and a Dickson Lab DIC/TA standard (for y-intercept of calibration).

Instrument Type

Generic Name: pH Sensor

Generic Description:

An instrument that measures the hydrogen ion activity in solutions.

The overall concentration of hydrogen ions is inversely related to its pH. The pH scale ranges from 0 to 14 and indicates whether acidic (more H+) or basic (less H+).

Thermometer

Supplied Name: High precision partial-immersion glass thermometer

Supplied Description:

High precision partial-immersion glass thermometer (precision ±0.3%; accuracy ±0.4%).

Instrument Type

Generic Name: Thermometer

Generic Description:

A device designed to measure temperature.

YSI Professional Plus Multi-Parameter Probe

Supplied Name: YSI 3200 (Yellow Springs, Ohio, USA)

Supplied Description:

YSI 3200 (Yellow Springs, Ohio, USA) conductivity probe with a cell constant of k = 10.

Instrument Type

Generic Name: YSI Professional Plus Multi-Parameter Probe

Acronym: YSI ProPlus

Generic Description:

The YSI Professional Plus handheld multiparameter meter provides for the measurement of a variety of combinations for dissolved oxygen, conductivity, specific conductance, salinity, resistivity, total dissolved solids (TDS), pH, ORP, pH/ORP combination, ammonium (ammonia), nitrate, chloride and temperature. More information from the manufacturer.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Date	Date of sampling measurement Format: %Y-%m-%d	unitless	date
Group_ID	Cominations of experimental conditions and Tank number used to create a unique identifier for groups within the experiment	unitless	exp_id
Mean_pCO2	Mean pCO2 of experimental tank	uatm	pCO2
pCO2	pCO2 of the experimental tank	uatm	pCO2
pH	pH of the experimental tank	unitless	pH
Mean_pH	Mean pH of experimental tank	unitless	pH
Salinity	Salinity of the experimental tank	psu	sal
Tank	Tank number within a temperature/pCO2 combination	unitless	tank
Mean_Temp	Mean temperature of experimental tank	degrees Celsius	temp
Temp	Temperature of the experimental tank	degrees Celsius	temp

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Database

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Dataset: Measured experimental tank parameters