Dataset: Fe_dissolved_Ant1

Name: Dissolved iron from phyto and microzooplankton experiments from the RVIB Nathaniel B. Palmer NBP0601 cruise in the Ross Sea, Southern Ocean from 2005-2006 (CORSACS project, Antarctic microzooplankton project)
Published: 2014-02-06
Keywords: oceans

Cite This Dataset

Spatial Extent: N:-75 E:177.36 S:-75 W:177.36

Project:

Controls of Ross Sea Algal Community Structure (CORSACS)

Rising climatic temperatures impact on antarctic microzooplankton growth and grazing (Antarctic microzooplankton )

Program:

Ocean Carbon and Biogeochemistry (OCB)

Principal Investigator:

Dr Julie Rose (National Oceanic and Atmospheric Administration , NOAA-Milford)

Co-Principal Investigator:

David A. Hutchins (University of Southern California, USC)

BCO-DMO Data Manager:

Danie Kinkade (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

2014-02-06

Version Date:

2014-02-06

Validated:

Yes

Current State:

Final no updates expected

Dissolved iron from phyto and microzooplankton experiments from the RVIB Nathaniel B. Palmer NBP0601 cruise in the Ross Sea, Southern Ocean from 2005-2006 (CORSACS project, Antarctic microzooplankton project)

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Description

Experimental Design

Experiments were conducted during the CORSACS (Controls On Ross Sea Algal Community Structure) expedition in January 2006 to the Ross Sea, Antarctica, onboard the RVIB Nathaniel B. Palmer (cruise NBP-0601). Water was collected at 75.00S, 177.36E using a trace metal clean towed-intake surface water Teflon diaphragm pumping system (Bruland et al., 2005). Water was prescreened through acid-washed 200μm Nitex mesh to eliminate large zooplankton and collected into a 50-L mixing carboy. Collected water was gently mixed and dispensed into 12 4.5-L and 12 2.7-L acid washed trace metal clean clear polycarbonate bottles for incubation. Half of the bottles were spiked with 1.0nM FeCl3 (final concentration) at the beginning of the experiment. Bottles were incubated in two temperature controlled deck-board incubators (Feng et al., 2009; Hare et al., 2007). Incubators were screened to 18% of Io using two layers of neutral density filter. One incubator was kept at ambient temperature (0 deg C), while the temperature in the other was gradually increased to 4 deg C over the course of 24 h. Bottles were incubated for seven days. The 4.5-L bottles were sampled daily and the 2.7-L bottles were only sampled on the final day of the experiment. All sampling occurred under a laminar flow hood using trace metal clean techniques.

References

Bruland, K.W., E.L. Rue, G.J. Smith, and G.R. DiTullio. 2005. Iron, macronutrients and diatom blooms in the Peru upwelling regime: brown and blue waters of Peru. Marine Chemistry 93: 81-103.

Feng, Y., C.E. Hare, K. Leblanc, G.R. DiTullio, P.A. Lee, S.W. Wilhelm, J. Sun, J.M. Rose, N. Nemcek, I. Benner, and D.A. Hutchins. 2009. The effects of increased pCO2 and temperature on the North Atlantic Spring Bloom: I. The phytoplankton community and biogeochemical response. Marine Ecology Progress Series 388: 13-25.

Hare, C.E., K. Leblanc, G.R. DiTullio, R.M. Kudela, Y. Zhang, P.A. Lee, S.F. Riseman, and D.A. Hutchins. 2007. Consequences of increased temperature and CO2 for phytoplankton community structure in the Bering Sea. Marine Ecology Progress Series 352: 9-16.

Methods & Sampling

Total dissolved Fe concentrations were filtered through 0.4um track-etched polycarbonate filters (Nuclepore Whatman) and acidified to pH 1.7 with 4 mL -1 sub-boiled distilled HCl. Total dissolved Fe was measured using adsorptive cathodic stripping voltammetry (ACSV) (Buck et al., 2007). A 5mM salicylaldoxime (SA: Aldrich, ≥ 98%) solution was first prepared in quartz-distilled methanol (Q-MeOH) and stored in the refrigerator. A final concentration of 25uM SA was used for total dissolved Fe measurements. A 1.5 M borate buffer was made in 0.4 mol L quartz-distilled ammonium hydroxide (Q-NH4OH) as previously described (Ellwood and Van den Berg, 2000). The voltammetric system consisted of Princeton Applied Research (PAR) 303A interfaced with a computer-controlled AutolabII potentiostat/galvanostat (Eco Chemie). The working electrode was a “large” mercury drop (2.8 mm2), the reference electrode was Ag: saturated AgCl, saturated KCl, and the counterelectrode was a platinum wire. Acidified samples were microwaved 2×15 s at 1100W to release dissolved Fe from ambient organic ligands (Bruland et al., 2005), neutralized once with cool 1 mol L -1 QNH4OH, and buffered to pH 8.2 with the borate buffer. Once buffered, Fe and SA additions were made and following ACSV analysis Fe concentrations were determined from a linear regression of the standard addition curve. The detection limit for the ACSV method is 0.02 nmol L -1, calculated from three times the standard deviation of a 0.05 nmol L -1 Fe addition, as no peak is observed in either Milli-Q or ultraviolet (UV)-oxidized seawater (from which trace metals and metal-chelating organic ligands are removed from seawater; (Donat and Bruland, 1988) at deposition times of up to 600 s. Deposition times for sample analyses here were between 60 and 400 s, depending on ambient Fe concentrations. The precision of this technique for replicate samples is less than 4%.

Data Processing Description

BCO-DMO Processing Notes:

- File was sorted by treatment
- Added lat,lon values of original water sampling location to file
- Added BCO-DMO header lines
- Parameter names were edited to conform with BCO-DMO convention
- Added 'nd' for missing data

Data Files

File
disslvd_Fe_ant1.csv (Comma Separated Values (.csv), 244 bytes) MD5:e1be48d0dff48ab7b51510b30d791c80 Primary data file for dataset ID 490492

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Antarctic Sciences	PLR-0528715

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
NBP0601	CORSACS I	2005-12-17	RVIB Nathaniel B. Palmer	Giacomo DiTullio (Chief Scientist)	data info

Instruments

Voltammetry Analyzers

Supplied Description:

The voltammetric system consisted of Princeton Applied Research (PAR) 303A interfaced with a computer-controlled AutolabII potentiostat/galvanostat (Eco Chemie). The working electrode was a “large” mercury drop (2.8mm2), the reference electrode was Ag: saturated AgCl, saturated KCl, and the counterelectrode was a platinum wire.

Instrument Type

Generic Name: Voltammetry Analyzers

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB35/

Generic Description:

Instruments that obtain information about an analyte by applying a potential and measuring the current produced in the analyte.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Fe	Dissolved iron concentration.	nM	Fe
lat	Latitude component of geographic position where water was sampled.	decimal degrees	lat
lon	Longitude component of geographic position where water was sampled.	decimal degrees	lon
error_analytic	Analytical error in iron measurement	nM	no_bcodmo_term
bottle	Experimental bottle number.	dimensionless	no_bcodmo_term
day	Sampling day during experiment. The experiment was conducted during January, 2006.	dimensionless	time_inc
treatment	Experimental conditions varied during the experiment. Four treatments were used: Low temperature, low iron (LTLF); low temperature, high iron (LTHF); high temperature, low iron (HTLF); high temperature, high iron(HTHF)	dimensionless	treatment

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Database

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Dataset: Fe_dissolved_Ant1