Dataset: Trichodesmiium field metaproteomes - sample provenance

Name: Trichodesmium sample provenance from samples collected in North Atlantic surface waters, station BATS, and station ALOHA between 2000 and 2018
Published: 2020-01-10
Keywords: Trichodesmium, metaproteomics, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.787093.1

Spatial Extent: N:31.4 E:-21.59 S:0.17976 W:-158

Temporal Extent: 2000-07-27 - 2018-03-11

Project:

Collaborative Research: Iron and phosphorus balanced limitation of nitrogen fixation in the oligotrophic ocean (TriCoLim)

Collaborative Research: Evolutionary, biochemical and biogeochemical responses of marine cyanobacteria to warming and iron limitation interactions (Cyanobacteria Warming Responses)

Marine Microbial Investigator Award: Investigator Mak Saito (MM Saito)

New technology for high resolution analysis of proteins and other organic materials produced by marine microorganisms (MM Proteins and Organics Tech)

Program:

Marine Microbiology Initiative (MMI)

Principal Investigator:

Mak A. Saito (Woods Hole Oceanographic Institution, WHOI)

Student:

Noelle Held (Woods Hole Oceanographic Institution, WHOI)

BCO-DMO Data Manager:

Amber D. York (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2020-01-10

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Trichodesmium sample provenance from samples collected in North Atlantic surface waters, station BATS, and station ALOHA between 2000 and 2018

Abstract:

Trichodesmium sample provenance collected in North Atlantic surface waters, at station BATS (Bermuda Atlantic Time-series Study), and station ALOHA (A Long-Term Oligotrophic Habitat Assessment) between 2000 and 2018.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2020-01-10	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.787093.1	2020-04-02

Description

Related Datasets:
Trichodesmium Metaproteomes: https://www.bco-dmo.org/dataset/787078
Trichodesmium single colonies metaproteomes: https://www.bco-dmo.org/dataset/786694
Trichodesmium field metaproteomes - peptide spectral counts: https://www.bco-dmo.org/dataset/787168
Trichodesmium field metaproteomes - protein spectral counts: https://www.bco-dmo.org/dataset/787147
Trichodesmium field metaproteomes - sequence fasta: https://www.bco-dmo.org/dataset/787181

Methods & Sampling

A 200mm plankton net was deployed to ~20m depth, then recovered to just below surface, repeating five times. Trichodesmium colonies were hand-picked into 0.2mm filtered surface seawater, rinsed twice in 0.2mm filtered surface seawater, and decanted onto a 0.2-4mm Supor filter.

Data Processing Description

BCO-DMO Data Manager Processing Notes:

* added a conventional header with dataset name, PI name, version date

* modified parameter names to conform with BCO-DMO naming conventions (spaces, +, and - changed to underscores). Units in parentheses removed and added to Parameter Description metadata section.

* blank values in this dataset are displayed as "nd" for "no data." nd is the default missing data identifier in the BCO-DMO system.

* micron unit characters "uM" removed from filter size values in the data table and added to the units in parameter description section.

* Date format changed to ISO 8601 format yyyy-mm-dd
* As discussed with PI, if latitude and longitude are integers, use one decimal place of precision. So .0 added to integer lat/lons. Locations were intentionally selected to fall on integer degrees in some cases.

Data Files

File
samples.csv (Comma Separated Values (.csv), 1.39 KB) MD5:efa6081141eb7146c5376c1fdf4b5773 Primary data file for dataset ID 787093

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1657766
NSF Division of Ocean Sciences	OCE-1850719
Gordon and Betty Moore Foundation: Marine Microbiology Initiative	GBMF3934
Gordon and Betty Moore Foundation: Marine Microbiology Initiative	GBMF3782

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
AT39-05	TriCoLim	2018-02-11	R/V Atlantis	David A. Hutchins (Chief Scientist)	data info
JC150	Ziploc	2017-06-26	RRS James Cook	Claire Mahaffey (Chief Scientist)	data info

Instruments

None available yet

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Longitude	Longitude	decimal degrees	lon
Latitude	Latitude	decimal degrees	lat
Net_size	Plankton net mesh size	microns (um)	net_mesh
Filter_size	Supor filter size	microns (um)	filter_size
time_zone	Local time zone used for columns Date_sampled and Time_sampled.	unitless	time_zone
Name	Sample name	unitless	sample
Replication	Replication (singlicate\|duplicate\|triplicate)	unitless	sample_descrip
Cruise	Cruise identifier (or station BATS or HOT)	unitless	cruise_id
Cruise_station_num	Station number	unitless	station
Time_sampled	Description of time sampled	unitless	sample_descrip
Date_sampled	Date of sample	unitless	date