Dataset: Laboratory results: Raw mass spectrometer data from a diatom (Thalassiosira pseudonana) grown under CO2-replete and CO2-stressed conditions; conducted in the Kustka lab at Rutgers from 2007-2013

ValidatedFinal no updates expectedVersion 25 March 2014 (2014-03-25)Dataset Type:Unknown

Principal Investigator, Contact: Adam Kustka (Rutgers University)

Co-Principal Investigator: Kay D. Bidle (Rutgers University)

BCO-DMO Data Manager: Shannon Rauch (Woods Hole Oceanographic Institution)


Project: A Matter of Life or Death? Assessing the physiological roles of PCD-related genes to stress adaptation in diatoms (Diatom PCD genes)

These data consist of the raw mass spectrometry files collected as part of an effort to understand the proteomic response of the marine diatom Thalassiosira pseudonana to varied CO2 concentration. High and low CO2 acclimated cells were grown with 15N-nitrate or natural abundance nitrate and harvested for proteomic analysis.

Data consist of 37 gigabytes of .raw files produced by the Thermo Scientific mass spectrometer. To obtain the data, please contact BCO-DMO.

Description of the data files:
The data files (37 GB in total size) are in .raw format, as produced by the Thermo Scientific mass spectrometer. The investigators used Proteome Discoverer software to analyze the .raw files. The mass spectrometry files are stored under in folders named MS3027 and MS3184, which were collected for the first and second biological replicates, respectively. In cases where individual LC fractions were subject to mass spectrometry multiple times, file names for the repeated analyses are the VLS number followed by "_<n>", where n represents the replicate analysis.  Further details for data acquisition and filtering can be found at Kustka et al. 2014 (in second review; New Phytologist).


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