Dataset: CO2-stressed diatom proteome
Deployment: lab_Kustka

Raw mass spec data from a diatom (T. pseudonana) grown under CO2-replete & CO2-stressed conditions. Data will be made available upon request.
View Data: For data, See Dataset Metadata Page:
Principal Investigator: 
Adam Kustka (Rutgers University)
Co-Principal Investigator: 
Kay D. Bidle (Rutgers University, Rutgers IMCS)
Adam Kustka (Rutgers University)
BCO-DMO Data Manager: 
Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)
Current State: 
Final no updates expected
14 Jan 2014
Version Date: 

These data consist of the raw mass spectrometry files collected as part of an effort to understand the proteomic response of the marine diatom Thalassiosira pseudonana to varied CO2 concentration. High and low CO2 acclimated cells were grown with 15N-nitrate or natural abundance nitrate and harvested for proteomic analysis.

Data consist of 37 gigabytes of .raw files produced by the Thermo Scientific mass spectrometer. To obtain the data, please contact BCO-DMO.

Description of the data files:
The data files (37 GB in total size) are in .raw format, as produced by the Thermo Scientific mass spectrometer. The investigators used Proteome Discoverer software to analyze the .raw files. The mass spectrometry files are stored under in folders named MS3027 and MS3184, which were collected for the first and second biological replicates, respectively. In cases where individual LC fractions were subject to mass spectrometry multiple times, file names for the repeated analyses are the VLS number followed by "_<n>", where n represents the replicate analysis.  Further details for data acquisition and filtering can be found at Kustka et al. 2014 (in second review; New Phytologist).

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