Dataset: T-RFLP, response of the microbial community to coral spawning, lagoon and reef flats in Kaneohe Bay, Oahu, Hawaii during 2006 - 2007 (MiCoDe project)

ValidatedFinal no updates expectedVersion 2015-03-12 (2015-03-12)Dataset Type:Unknown

Principal Investigator: Michael S. Rappé (University of Hawaiʻi at Mānoa)

Contact: Amy Apprill (University of Hawaiʻi at Mānoa)

BCO-DMO Data Manager: Nancy Copley (Woods Hole Oceanographic Institution)


Project: The Development of Microbial Associations in Major Reef Building Corals of the Pacific Ocean (MiCoDe)

The response of the microbial community to coral spawning was investigated over a period of 18 mo, from January 2006 to July 2007, in reef flat and lagoon environments of a subtropical embayment (Kaneohe Bay, Oahu, Hawaii, USA). The composition of the bacterioplankton community was characterized using terminal restriction fragment length polymorphism (T-RFLP) analysis of bacterial small-subunit (SSU) ribosomal RNA genes in parallel with measurements of microbial cell abundances, bacterial production via 3H-leucine incorporation, and seawater biochemical parameters. Abundance of coral eggs, zooplankton, and bacterial, and chlorophyll were measured along with nutrient analysis and physical parameters.

Related Reference:

These data are published in Apprill, A. M., and M. S. Rappé (2011) Response of the microbial community to coral spawning in lagoon and reef flat environments of Hawaii, USA. Aquatic Microbial Ecology, 62:251-266.


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