Dataset: DHAB halocline sediment pyrotags - eukaryotes
Deployment: AT18-14

454 pyrotags for Eukaryote taxa in deep hypersaline anoxic basin (DHAB) halocline sediments
Principal Investigator: 
Joan M. Bernhard (Woods Hole Oceanographic Institution, WHOI)
Virginia P. Edgcomb (Woods Hole Oceanographic Institution, WHOI)
BCO-DMO Data Manager: 
Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)
Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)
Current State: 
Final no updates expected
Version Date: 

Pyrotags of eukaryotes obtained from Urania, Discovery, and L’Atalante DHAB halocline sediments.

Small subunit ribosomal RNA V4 hyper variable region eukaryotic pyrotags prepared from total RNA extracted from sediments below haloclines of 3 deep hypersaline anoxic basins in the Eastern Mediterranean Sea and from nearby control sites (normal saline deep marine sediments in close proximity to deep hypersaline anoxic basins). Total RNA was transcribed to cDNA, and eukaryotic V4 pyrotags amplified from cDNA.

Related References:
Bernhard, J.M., CM Morrison, E Pape, DJ Beaudoin, MA Todaro, MG Pachiadaki, K Ar Kormas, & VP Edgcomb. 2015 (submitted). Metazoans of redoxcline seidments in Mediterranean deep-sea hypersaline anoxic basins.

Bernhard, J.M., Kormas, K.A., Pachiadaki, M.G., Rocke, E., Beaudoin, D.J., Morrison, C., Visscher, P.T., Cobban, A., Starczak, V.R., and Edgcomb, V.P. 2014. Benthic protists and fungi of Mediterranean deep hypersaline anoxic basin redoxcline sediments. Frontiers in Microbiology, doi: 10.3389/fmicb.2014.00605.

More information about this dataset deployment