Dataset: Nutrients and Targeted Proteomics
Deployment: KM1128

Macronutrients from the trace metal rosette and targeted metaproteomics analyzed by triple quadrupole mass spectrometry calibrated with stable isotope labeled peptides.
Principal Investigator: 
Mak A. Saito (Woods Hole Oceanographic Institution, WHOI)
BCO-DMO Data Manager: 
Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)
Current State: 
Final with updates expected
Deployment Synonyms:
 MetZyme
Version: 
01 August 2017
Version Date: 
2017-08-01
Description

This dataset includes data from 3 sampling systems:
- Macronutrients from the trace metal rosette (TMR); analyzed by Joe Jennings at OSU.
- Targeted metaproteomics from the McLane pumps; analyzed by triple quadrupole mass spectrometry calibrated with stable isotope labeled peptides.
- Pigments from a combination of CTD and TMR samples; determined by HPLC; analyzed by Jack DiTullio, University of Charleston. Note that pigments were collected by both CTD and TMR due to a loss of the CTD as station 2/3.

Samples were collected during the KM1128 METZYME research expedition (Metals and Enzymes in the Pacific) on the R/V Kilo Moana October 1-25, 2011 from Oahu, Hawaii to Apia, Samoa.

01 Aug 2017: dataset was revised to include columns for date/time and cast numbers for each sampling system.

Related publications:
Saito, M. A., M. R. McIlvin, D. M. Moran, T. J. Goepfert, G. R. DiTullio, A. F. Post, and C. H. Lamborg. 2014. Multiple nutrient stresses at intersecting Pacific Ocean biomes detected by protein biomarkers. Science 345:1173-1177. DOI: 10.1126/science.1256450

Saito, M. A.; Dorsk, A.; Post, A. F.; McIlvin, M.; Rappé, M. S.; DiTullio, G.; Moran, D. 2015.  Needles in the Blue Sea: Sub‐Species Specificity in Targeted Protein Biomarker Analyses Within the Vast Oceanic Microbial Metaproteome. Proteomics. DOI: 10.1002/pmic.201400630

More information about this dataset deployment