Dataset: Nitrification Rates
Deployment: KM1128

Rates of water column nitrification determined from bottle incubations with 15N tracers (15NH4Cl).

Principal Investigator:

Alyson E. Santoro (University of California-Santa Barbara, UCSB)

BCO-DMO Data Manager:

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Connecting Trace Elements and Metalloenzymes Across Marine Biogeochemical Gradients (GPc03) (MetZyme)

Gene content, gene expression, and physiology in mesopelagic ammonia-oxidizing archaea (AmoA Archaea)

Current State:

Final no updates expected

Version:

27 May 2016

Deployment Synonyms:

GPc03, MetZyme

Version Date:

2016-05-27

Data URL:

https://www.bco-dmo.org/dataset-deployment/647797/data

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Description

Rates of water column nitrification determined from bottle incubations with 15N tracers (15NH4Cl); sample collected on METZYME cruise (KM1128).

Related references:
Santoro, A.E., Casciotti, K.L., and C.A. Francis. 2010. Activity, abundance,and diversity of nitrifying archaea and bacteria in the central California Current. Environmental Microbiology 12: 1989-2006. doi:10.1111/j.1462-2920.2010.02205.x

Santoro, A.E., Saito, M.A., Goepfert, T.J., Lamborg, C.H., Dupont, C.L., G.R. DiTullio. Thaumarchaeal ecotype distributions across the equatorial Pacific and their potential roles in nitrification and sinking flux attenuation. Submitted to Limnology & Oceanography, April 2016.

Methods & Sampling

Dataset acquisition description

Nitrification rate incubations were conducted at Stations 1, 3, and 5 at four depths and followed procedures described in Santoro et al. 2010, Environ. Micro. Timecourse samples of d15NNOx were analyzed by isotope ratio mass spectrometer using the ‘denitrifier method.’ Complete methods specific to the MetZyme cruise dataset deposited here are described in Santoro et al., submitted to L&O.

Rates for Station 1 were initiated from the ship’s rosette; Stations 3 and 5 used a trace metal clean rosette.

Data Processing Description

Dataset Processing Description

The MATLAB script ‘metzyme_rates.m’ was used with the raw data files ('initials...' and ‘data...'). Compiled rates were deposited in a tab-delimited text file.

MATLAB scripts: (click to view files or right-click to save)
metzyme_rates.m
nitr_rate_rev.m

Raw data: (click to view files or right-click to save)
initials_mzst1.csv
initials_mzst3.csv
initials_mzst5.csv
data_mzst1.csv
data_mzst3.csv
data_mzst5.csv

BCO-DMO Processing:
- Modified paramter names to conform with BCO-DMO naming conventions;
- Added cruise ID.

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1031271	NSF Division of Ocean Sciences
OCE-1260006	NSF Division of Ocean Sciences

Instruments

None available yet

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
cruise	Cruise identifier.	unitless	cruise_id
cast	Cast number. TMR = Trace Metal Rosette. CTD = Ship's CTD.	unitless	cast
station	Station number.	unitless	station
lon	Longitude east.	decimal degrees	lon
lat	Latitude north.	decimal degrees	lat
depth	Sample depth.	meters (m)	depth
bottle	Incubation bottle number (1,2 = experimental bottles; 3 = no addition control)	unitless	bottle
nitr_rate	Nitrification rate.	nanomoles per liter per day (nmol L-1 d-1)	no_bcodmo_term
nitr_rate_SE	Standard error of the rate fit.	nanomoles per liter per day (nmol L-1 d-1)	std_err

Database

Contribute Data

Dataset: Nitrification Rates
Deployment: KM1128

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Nitrification RatesDeployment: KM1128

Dataset acquisition description

Dataset Processing Description

Dataset: Nitrification Rates
Deployment: KM1128