Dataset: EN584 LV incubation, peptidase and glucosidase activities
Deployment: EN584

Large volume incubation, peptidase and glucosidase activities (plate reader), EN584
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Principal Investigator: 
Carol Arnosti (University of North Carolina at Chapel Hill, UNC-Chapel Hill)
BCO-DMO Data Manager: 
Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Fluorescence was measured over 24-48 hours incubation time with a plate reader (TECAN spectrafluor plus; 360 nm excitation, 460 emission), with time points taken every 4-6 hours. Hydrolysis rates were calculated from the rate of increase of fluorescence in the incubation over time relative to a set of standards of known concentration of fluorophore. Scripts to calculate hydrolysis rates and produce the figures shown here are available in the associated Github repository [Hoarfrost, 2017].

See Niskin Bottle and Cast List EN584 to link specific casts and bottles to each experiment:

More information about this dataset deployment