Dataset: 16S rRNA gene from DNA
Deployment: Chikyu-331

16S rRNA gene (from DNA)

Principal Investigator:

Christopher House (Pennsylvania State University, PSU)

Scientist:

Leah Brandt (Pennsylvania State University, PSU)

BCO-DMO Data Manager:

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

An In-Depth analysis of the subvent biosphere within Okinawa Backarc Basin (IODP 331, Iheya North Hydrothermal Field) sediments (Subvent_Biosphere_Sediments)

Current State:

Final no updates expected

Deployment Synonyms:

IODP-331

Data URL:

https://www.bco-dmo.org/dataset-deployment/780935/data

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Description

Next-generation sequencing data across a hydrothermal gradient; Okinawa Backarc Basin IODP 331 – subvent biosphere. The purpose of this dataset was to investigate taxonomic changes via the 16S rRNA gene from extractable DNA across 45 meters of recovered core. The temperature gradient in this section of the dynamic Iheya North Hydrothermal system was estimated to be ~3C/m. The interval transitions sharply from low-temperature marine mud to hydrothermally altered clay.

Methods & Sampling

Dataset acquisition description

Sampling of cores was done in accordance with IODP protocols.

All C0014 samples were cored on 16 September 2010 via via HPCS (hydraulic piston coring system).
All C0015 samples were cored on 18 September 2010 via HPCS.
All C0017 samples were cored on 27 September 2010 via HPCS.

Core sections were kept frozen (-80°C) until DNA extractions. A MoBio® Power Soil kit was used to extract environmental DNA. See publication below for sequencing and analysis methodologies.

Data Processing Description

Dataset Processing Description

Data Processing: Demultiplexing was performed in Mothur (v.1.30.1).

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-0939564	NSF Division of Ocean Sciences

Instruments

Piston Corer

Supplied Name: hydraulic piston coring system (HPCS)

Supplied Description:

Instrument Type

Generic Name: Piston Corer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/51/

Generic Description:

The piston corer is a type of bottom sediment sampling device. A long, heavy tube is plunged into the seafloor to extract samples of mud sediment. A piston corer uses a "free fall" of the coring rig to achieve a greater initial force on impact than gravity coring. A sliding piston inside the core barrel reduces inside wall friction with the sediment and helps to evacuate displaced water from the top of the corer. A piston corer is capable of extracting core samples up to 90 feet in length.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
Cruise_ID	Cruise ID number	unitless	cruise_id
IODP_Sample_Number	IODP sample number	unitless	sample
Latitude	Latitude; positive values = North	decimal degrees	lat
Longitude	Longitude; positive values = East	decimal degrees	lon
Water_depth	Water depth	meters	depth_w
Top_Depth	Top depth	meters below seafloor	depth_bsf
Bottom_Depth	Bottom depth	meters below seafloor	depth_bsf
Sediment_type	Sediment type	unitless	sample_descrip
Estimated_Temp	Estimated temperature; based on 3 degrees C/m	degrees Celsius	temp
MGRAST_Accession_ID	MG-RAST accession ID number	unitless	accession_number
Notes	Notes	unitless	comment

Database

Contribute Data

Dataset: 16S rRNA gene from DNA
Deployment: Chikyu-331

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: 16S rRNA gene from DNADeployment: Chikyu-331

Dataset acquisition description

Dataset Processing Description

Dataset: 16S rRNA gene from DNA
Deployment: Chikyu-331