Dataset: Trichodesmium AHL amendment metatranscriptomic reads accessions and metadata

ValidatedFinal no updates expectedDOI: 10.1575/1912/bco-dmo.746654.1Version 1 (2018-09-21)Dataset Type:Cruise Results

Principal Investigator: Sonya T. Dyhrman (Lamont-Doherty Earth Observatory)

Co-Principal Investigator: Benjamin A.S. Van Mooy (Woods Hole Oceanographic Institution)

Student: Kyle R. Frischkorn (Lamont-Doherty Earth Observatory)

BCO-DMO Data Manager: Nancy Copley (Woods Hole Oceanographic Institution)


Project: Dissolved Phosphorus Processing by Trichodesmium Consortia: Quantitative Partitioning, Role of Microbial Coordination, and Impact on Nitrogen Fixation (P Processing by Tricho)


Abstract

Trichodesmium is a marine, diazotrophic cyanobacterium that plays a central role in the biogeochemical cycling of carbon and nitrogen. Colonies ubiquitously co-occur with a diverse microbiome of heterotrophic bacteria. Here we show that manipulation of the microbiome with quorum sensing acyl homoserine lactone (AHL) molecules significantly modulated rates of N2 fixation by Trichodesmium collected from the western North Atlantic, with both positive and negative effects of varied magnitude. Change...

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The samples are composed of raw metatranscriptomic reads from acyl homoserine lactone (AHL) (quorum sensing) addition incubation experiments performed on Trichodesmium colonies collected in May 2014 on the 'PABST' cruise (R/V Atlantic Explorer AE1409) in the Sargasso Sea. We extracted prokaryotic RNA from triplicate control and +AHL samples, pooling together triplicate samples and sequencing 60 million paired end reads.

Links to the NCBI GenBank BioProjects are provided.

Raw reads can also be found on the NCBI SRA under accession code PRJNA450995.


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Results

Frischkorn, K. R., Rouco, M., Van Mooy, B. A. S., & Dyhrman, S. T. (2018). The Trichodesmium microbiome can modulate host N2 fixation. Limnology and Oceanography Letters, 3(6), 401–408. doi:10.1002/lol2.10092