Dataset: Chemical and biological data collected for surface and bottom samples from all five stations along the transect from Charlotte Harbor to offshore in southwest Florida over the four sampling periods post-hurricane Ian during 2023-2024

Sampling Plan: Four sampling efforts were conducted (May 2023, August 2023, September 2023, October 2024), focusing on five stations located from Charlotte Harbor to the adjacent inner West Florida Shelf (WFS) system directly impacted by Hurricane Ian and the resulting plumes using Mote's R/V Mote (42 ft). At each station, water column CTD profiles of temperature, salinity, dissolved oxygen, and pH were made, and discrete surface and bottom water samples were collected by rosette-mounted Niskin bottles and analyzed for the parameters and rates listed in Table 1. Sample processing occurred on board or immediately after return to the laboratory. Net community production and oxygen utilization rates were calculated using observed O2 differences at hour intervals after air-sea O2 corrections (e.g., Wang et al. 2017). The balance between ecosystem respiration and production, the net community production, yielded a measure of the net autotrophic or heterotrophic ecosystem state (Hopkinson and Smith 2005). The carbonate and nutrient suite and rate process measurements (including uptake and regeneration rates) and direct measurements of organic matter respiration allowed an assessment of how nutrient availability and forms influence hypoxia and how hurricane inputs influence carbonate chemistry in this system, specifically the connection between acidification and hypoxia (e.g. O2 vs. pH relationships).

Analytical Methods: See Table 1 (supplemental file).