Dataset: Meta-analytic data for tolerance plasticity study - literature review from 2019-2020

Studies which measure thermal tolerance plasticity typically collect organisms from nature and acclimate them to different temperatures for defined periods of time in the lab before measuring thermal tolerance. Important methodological considerations for these types of studies include (1) for how long and at what temperature organisms are acclimated, (2) how fast the temperature is changed, and (3) whether measurements are made on field collected or F1 individuals, given that parental conditions are known to influence thermal plasticity. To identify publications for use in our study, we followed PRISMA (Preferred Reporting Items for Systematic reviews and Meta-Analyses) guidelines (supplemental figure S1 in Barley et al. 2021). We searched titles, abstracts, and key words in Web of Science (Clarivate Analytics, Philadelphia, USA) using the following search string: (Thermal OR temperatures) AND (Lethal OR “Thermal tolerance” OR “Thermal limit” OR CTmax OR CTmin OR LT50 OR “freezing tolerance”) AND ("Local* Adapt*" OR "“Latitud* Var” OR Intraspecific). We conducted an initial literature search on 24 August 2019 and updated the search on 28 July 2020. We also added studies that we were aware of but were not returned in the literature search. We used the following criteria for inclusion, where each study must have: (1) reported new results of whole-organism upper thermal limit in degrees Celsius for at least 2 populations of the same species, (2) experimentally measured thermal tolerance after acclimating all individuals to at least two temperatures, (3) reported a measure of error for the thermal tolerance estimate, and (4) not measured tolerance of introduced species, hybrid lines, cultivars, domesticated species, or later generations of experimental laboratory populations (greater than F2). Although we initially included both cold and heat tolerance studies, we later excluded cold tolerance studies from our analysis due to insufficient data. Studies of cold tolerance, and additional studies that met criteria (1) and (2) but were excluded based on (3) and/or (4) are listed in supplemental table S1 in Barley et al. 2021. We screened 400 publications that measured thermal tolerances across populations and identified 20 studies to include in our meta-analysis, representing 19 species. The studies that were accepted after looking through titles and abstracts but were later excluded from our analysis are listed in Table S1 in Barley et al. 2021. We extracted data from each publication using relevant figures, tables, text, or supplementary material. In the case of figures, we used WebPlotDigitizer (version 4.3; Rohatgi 2020) to extract means and error estimates.