From the subsamples, all zooplankton were counted and identifed. Counts were then converted into number per cubic meter as follows: Number counted \/ (Subsample size X Amount filtered).<\/p>\n
It is worth noting that the frequency of repeated densities is an artifact of the small subsample volumes screened in order to process a large number of samples. The small sample volumes did not give us the ability to resolve small differences in abundance at individual species and stages (e.g., Cal fin Nauplius I). At this level of sorting many counts were reported as zeros, ones, twos or threes. These counts are at the threshold for detection, and the results give the false impression of many identical densities (concentrations). This also shows up in gaps in the life histories as well. For example, a single depth\/station might have NI and NIII and NIV stages, but not II or V. As a result, samples were often recombined to give the population characteristics of an integrated water column, or a group of samples. The approach worked well for our research objectives and we are well aware of its limitations. 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