{"@context":{"content":"http:\/\/purl.org\/rss\/1.0\/modules\/content\/","dc":"http:\/\/purl.org\/dc\/terms\/","foaf":"http:\/\/xmlns.com\/foaf\/0.1\/","og":"http:\/\/ogp.me\/ns#","rdfs":"http:\/\/www.w3.org\/2000\/01\/rdf-schema#","sioc":"http:\/\/rdfs.org\/sioc\/ns#","sioct":"http:\/\/rdfs.org\/sioc\/types#","skos":"http:\/\/www.w3.org\/2004\/02\/skos\/core#","xsd":"http:\/\/www.w3.org\/2001\/XMLSchema#","owl":"http:\/\/www.w3.org\/2002\/07\/owl#","rdf":"http:\/\/www.w3.org\/1999\/02\/22-rdf-syntax-ns#","rss":"http:\/\/purl.org\/rss\/1.0\/","site":"https:\/\/www.bco-dmo.org\/ns#","odo":"http:\/\/ocean-data.org\/schema\/","emo":"http:\/\/ocean-data.org\/schema\/entity-matching#","bibo":"http:\/\/purl.org\/ontology\/bibo\/","crypto":"http:\/\/id.loc.gov\/vocabulary\/preservation\/cryptographicHashFunctions\/","bcodmo":"http:\/\/lod.bco-dmo.org\/id\/","tw":"http:\/\/tw.rpi.edu\/schema\/","dcat":"http:\/\/www.w3.org\/ns\/dcat#","time":"http:\/\/www.w3.org\/2006\/time#","geo":"http:\/\/www.w3.org\/2003\/01\/geo\/wgs84_pos#","geosparql":"http:\/\/www.opengis.net\/ont\/geosparql#","sf":"http:\/\/www.opengis.net\/ont\/sf#","void":"http:\/\/rdfs.org\/ns\/void#","sd":"http:\/\/www.w3.org\/ns\/sparql-service-description#","dctype":"http:\/\/purl.org\/dc\/dcmitype\/","prov":"http:\/\/www.w3.org\/ns\/prov#","schema":"http:\/\/schema.org\/","geolink":"http:\/\/schema.geolink.org\/1.0\/base\/main#","spdx":"http:\/\/spdx.org\/rdf\/terms#","bcodmo_vocab":"http:\/\/schema.bco-dmo.org\/"},"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset\/3251#graph","@graph":[{"http:\/\/lod.bco-dmo.org\/id\/dataset\/3251":{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset\/3251","@type":["http:\/\/ocean-data.org\/schema\/DeploymentDatasetCollection","http:\/\/www.w3.org\/ns\/dcat#Dataset","http:\/\/ocean-data.org\/schema\/Dataset"],"http:\/\/ocean-data.org\/schema\/hasAcquisitionDescription":[{"@value":"
Methods for Phototrophic and Heterotrophic Plankton Taxa Sampling, RISE - Biomass of phytoplankton and microzooplankton taxa groups BCO-DMO Processing Notes<\/b> BCO-DMO Edits<\/b>
\nPreservation, Enumeration and Biomass Estimation
\n(Modified from Frame and Lessard, in press)<\/b>
\nBiomass of phytoplankton and microzooplankton taxa groups were measured
\nin the initial samples for dilution experiments off the Washington and
\nOregon coasts taken during four RISE cruises 2004-2006.
\nReplicate samples for phytoplankton and microzooplankton identification,
\nenumeration, and sizing were preserved with either acid Lugol's (5% final
\nconc.) or gluteraldehyde (0.5% final conc.). Gluteraldehyde-fixed samples
\nwere stained with DAPI and proflavin, then filtered onto 0.8\u00b5m and 0.2\u00b5m
\nblack polycarbonate membranes (Lessard and Murrell 1996). Slides were
\nstored frozen until analysis onshore.
Microscope counts<\/i>
\nKnown volumes of the Lugols-preserved samples were settled in counting
\nchambers for enumeration of ciliates (including mixotrophs), diatoms and
\nlarger dinoflagellates. Counts were performed on a Zeiss inverted microscope
\nat 250x magnification. Glutarldehyde-preserved samples on 0.8\u00b5m filters
\nwere examined with a Zeiss standard epifluorescence microscope at 400x
\nmagnification for enumeration of microphytoplankton including photosynthetic
\ndinoflagellates (typically
\nraphidophyte. Samples on the 0.2\u00b5m filters were examined at 1000x
\nmagnification for cyanobacteria, picoeukaryotes (
\nand heterotrophic nanoflagellates (>2-10\u00b5m).
Biomass estimates<\/i>
\nPicoplankton (cyanobacteria and picoeukaryotes) were sized using images
\ntaken with a QImaging Retiga EX charged coupled device (CCD) camera and
\nImage Pro Plus software. Dimensions of all other cells were measured
\nusing a computer-aided digitizing system (Roff and Hopcroft 1986).
\nCell volumes were calculated using appropriate geometric formulas and
\ncarbon was calculated using the carbon to volume equations of Menden-Deuer
\nand Lessard (2000) for diatoms, nanoplankton and dinoflagellates, and of
\nWorden et al. (2004) for picoplankton. Ciliate carbon was estimated using
\nthe conversion from Putt and Stoecker (1989).
References for further details:<\/b>
\nLessard, E.J. and M.C. Murrell (1996), Distribution, abundance and size composition
\nof heterotrophic dinoflagellates and ciliates in the Sargasso Sea near Bermuda,
\nDeep Sea Research Part I, 43(7), 1045-1065.
\nFrame, E.R. Frame and E.J. Lessard. Does the Columbia River Plume influence phytoplankton
\ncommunity structure along the Washington and Oregon coasts? In press. J. Geophysical Res.
\nMenden-Deuer, S., and E. J. Lessard (2000), Carbon to volume relationships for
\ndinoflagellates, diatoms, and other protist plankton. Limnol. Oceanogr., 45, 569-579.
\nPutt, M., and D. K. Stoecker. 1989. An experimentally determined carbon:volume ratio
\nfor marine \"oligotrichous\" ciliates from estuarine and coastal waters.
\nLimnol. Oceanogr. 34: 1097-1103.
\nRoff, J. C. and R. R. Hopcroft (1986), High precision microcomputer based measuring
\nsystem for ecological research, Can. J. Fish. Aquat. Sci., 43, 2044-2048.
\nWorden, A.Z., J.K. Nolan, and B. Palenik (2004), Assessing the dynamics and ecology
\nof marine picophytoplankton: the importance of the eukaryotic component,
\nLimnol. Oceanogr., 49(1), 168-179.
<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/ocean-data.org\/schema\/hasBriefDescription":[{"@value":"Biomass of phytoplankton and microzooplankton taxa groups","@language":"en-US"}],"http:\/\/purl.org\/dc\/terms\/description":[{"@value":"
<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/www.w3.org\/2000\/01\/rdf-schema#label":[{"@value":"Biomass","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/hasProcessingDescription":[{"@value":"
\nGenerated from original single sheet xls file contributed to BCO-DMO by Evelyn Lessard
<\/p>\n
\n - spaces in Cruise text field converted to \"_\"
\n - Date formatted to YYYYMMDD
\n - decimal data values padded to consistent decimal places
\n - Cruise changed from \"RISE_1,2,3,4\" to RISE04W1, etc for consistency with other data sets
<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/purl.org\/dc\/terms\/identifier":[{"@value":"3251","@type":"xsd:int"}],"http:\/\/purl.org\/dc\/terms\/title":[{"@value":"Biomass"}],"http:\/\/purl.org\/dc\/terms\/date":[{"@value":"2010-08-23T10:07:03-04:00","@type":"xsd:dateTime"}],"http:\/\/purl.org\/dc\/terms\/created":[{"@value":"2010-08-23T10:07:03-04:00","@type":"xsd:dateTime"}],"http:\/\/purl.org\/dc\/terms\/modified":[{"@value":"2023-07-07T16:10:26-04:00","@type":"xsd:dateTime"}],"http:\/\/rdfs.org\/ns\/void#inDataset":[{"@id":"http:\/\/www.bco-dmo.org\/"}],"http:\/\/ocean-data.org\/schema\/namedGraph":[{"@value":"urn:bcodmo:dataset:3251","@type":"xsd:token"}],"http:\/\/ocean-data.org\/schema\/osprey_page":[{"@id":"https:\/\/www.bco-dmo.org\/dataset\/3251"}],"http:\/\/ocean-data.org\/schema\/identifier":[{"@value":"_:Identifier3251"}],"http:\/\/ocean-data.org\/schema\/datasetTitle":[{"@value":"Phytoplankton and microzooplankton taxonomy biomass from R\/V Wecoma multiple cruises in the Northeast Pacific coastal waters off states of Washington and Oregon from 2004 to 2006 (RISE project)","@language":"en-US"}],"http:\/\/ocean-data.org\/schema\/abstract":[{"@value":"","@language":"en-US"}],"http:\/\/purl.org\/dc\/terms\/rights":[{"@id":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"http:\/\/ocean-data.org\/schema\/deprecated":[{"@value":"false","@type":"xsd:boolean"}],"http:\/\/ocean-data.org\/schema\/spatialCoverage":[{"@value":"_:spatialCoverage3251"}],"http:\/\/purl.org\/dc\/terms\/bibliographicCitation":[{"@value":"Lessard, E. J. (2009) Phytoplankton and microzooplankton taxonomy biomass from R\/V Wecoma multiple cruises in the Northeast Pacific coastal waters off states of Washington and Oregon from 2004 to 2006 (RISE project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 20 March 2009) Version Date 2009-03-20 [if applicable, indicate subset used]. http:\/\/lod.bco-dmo.org\/id\/dataset\/3251 [access date]","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/validated":[{"@value":"true","@type":"xsd:boolean"}],"http:\/\/ocean-data.org\/schema\/versionLabel":[{"@value":"20 March 2009","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/currentState":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-current-state\/7"}],"http:\/\/ocean-data.org\/schema\/nodcTopic":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/nodc-dataset-topic\/156"}],"http:\/\/ocean-data.org\/schema\/restricted":[{"@value":"false","@type":"xsd:boolean"}],"http:\/\/ocean-data.org\/schema\/hasAward":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/award\/54893"}],"http:\/\/ocean-data.org\/schema\/storesValuesFor":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-parameter\/19920"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-parameter\/19921"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-parameter\/19922"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-parameter\/19923"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-parameter\/19924"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-parameter\/19925"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-parameter\/19926"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-parameter\/19927"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-parameter\/19928"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-parameter\/19929"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-parameter\/19930"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-parameter\/19931"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-parameter\/19932"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-parameter\/19933"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-parameter\/19934"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-parameter\/19935"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset-parameter\/19936"}],"http:\/\/ocean-data.org\/schema\/hasAgentWithRole":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/person-role\/464272"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/person-role\/464273"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/person-role\/464274"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/person-role\/464271"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/person-role\/462075"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/person-role\/462077"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/person-role\/462079"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/person-role\/462081"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/person-role\/462076"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/person-role\/462078"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/person-role\/462080"},{"@id":"http:\/\/lod.bco-dmo.org\/id\/person-role\/462082"}],"http:\/\/purl.org\/dc\/terms\/language":[{"@value":"http:\/\/id.loc.gov\/vocabulary\/iso639-1\/en","@type":"xsd:anyURI"}],"http:\/\/xmlns.com\/foaf\/0.1\/homepage":[{"@id":"https:\/\/www.bco-dmo.org\/dataset\/3251"}],"http:\/\/purl.org\/dc\/terms\/issued":[{"@value":"2009-03-20","@type":"xsd:date"}],"http:\/\/purl.org\/dc\/terms\/publisher":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/affiliation\/191"}],"http:\/\/www.w3.org\/ns\/dcat#contactPoint":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/person\/50463"}]},"_:html3251":{"@id":"_:html3251","@type":["http:\/\/ocean-data.org\/schema\/MetadataViewAffordance"],"http:\/\/schema.org\/subjectOf":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset\/3251"}],"http:\/\/schema.org\/name":[{"@value":"HTML","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/affordedBy":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/affiliation\/191"}],"http:\/\/schema.org\/target":[{"@value":"_:html3251entryPoint"}]},"_:html3251entryPoint":{"@id":"_:html3251entryPoint","@type":["http:\/\/schema.org\/EntryPoint","http:\/\/ocean-data.org\/schema\/HtmlLandingPage"],"http:\/\/schema.org\/url":[{"@value":"https:\/\/www.bco-dmo.org\/dataset\/3251","@type":"xsd:anyURI"}],"http:\/\/schema.org\/contentType":[{"@value":"text\/html","@type":"xsd:token"}]},"_:frictionlessdata3251":{"@id":"_:frictionlessdata3251","@type":["http:\/\/ocean-data.org\/schema\/MetadataDownloadAffordance"],"http:\/\/schema.org\/subjectOf":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset\/3251"}],"http:\/\/schema.org\/name":[{"@value":"Datapackage.json","@type":"xsd:string"}],"http:\/\/schema.org\/alternateName":[{"@value":"Frictionless Data Package","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/affordedBy":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/affiliation\/191"}],"http:\/\/schema.org\/target":[{"@value":"_:frictionlessdata3251entryPoint"}]},"_:frictionlessdata3251entryPoint":{"@id":"_:frictionlessdata3251entryPoint","@type":["http:\/\/schema.org\/EntryPoint"],"http:\/\/schema.org\/url":[{"@value":"https:\/\/www.bco-dmo.org\/dataset\/3251\/datapackage.json","@type":"xsd:anyURI"}],"http:\/\/schema.org\/contentType":[{"@value":"application\/vnd.datapackage+json","@type":"xsd:token"}]},"_:datasetdescription3251":{"@id":"_:datasetdescription3251","@type":["http:\/\/ocean-data.org\/schema\/MetadataDownloadAffordance"],"http:\/\/schema.org\/subjectOf":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset\/3251"}],"http:\/\/schema.org\/name":[{"@value":"PDF","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/affordedBy":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/affiliation\/191"}],"http:\/\/schema.org\/target":[{"@value":"_:datasetdescription3251entryPoint"}]},"_:datasetdescription3251entryPoint":{"@id":"_:datasetdescription3251entryPoint","@type":["http:\/\/schema.org\/EntryPoint","http:\/\/ocean-data.org\/schema\/PdfDatasetDescription"],"http:\/\/schema.org\/url":[{"@value":"https:\/\/www.bco-dmo.org\/dataset\/3251\/Dataset_description.pdf","@type":"xsd:anyURI"}],"http:\/\/schema.org\/contentType":[{"@value":"application\/pdf","@type":"xsd:token"}]},"_:json3251":{"@id":"_:json3251","@type":["http:\/\/ocean-data.org\/schema\/MetadataDownloadAffordance"],"http:\/\/schema.org\/subjectOf":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset\/3251"}],"http:\/\/schema.org\/name":[{"@value":"JSON-LD","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/affordedBy":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/affiliation\/191"}],"http:\/\/schema.org\/target":[{"@value":"_:json3251entryPoint"}]},"_:json3251entryPoint":{"@id":"_:json3251entryPoint","@type":["http:\/\/schema.org\/EntryPoint"],"http:\/\/schema.org\/url":[{"@value":"https:\/\/www.bco-dmo.org\/dataset\/3251.json","@type":"xsd:anyURI"}],"http:\/\/schema.org\/contentType":[{"@value":"application\/ld+json","@type":"xsd:token"}]},"_:ttl3251":{"@id":"_:ttl3251","@type":["http:\/\/ocean-data.org\/schema\/MetadataDownloadAffordance"],"http:\/\/schema.org\/subjectOf":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset\/3251"}],"http:\/\/schema.org\/name":[{"@value":"Turtle","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/affordedBy":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/affiliation\/191"}],"http:\/\/schema.org\/target":[{"@value":"_:ttl3251entryPoint"}]},"_:ttl3251entryPoint":{"@id":"_:ttl3251entryPoint","@type":["http:\/\/schema.org\/EntryPoint"],"http:\/\/schema.org\/url":[{"@value":"https:\/\/www.bco-dmo.org\/dataset\/3251.ttl","@type":"xsd:anyURI"}],"http:\/\/schema.org\/contentType":[{"@value":"text\/turtle","@type":"xsd:token"}]},"_:rdf3251":{"@id":"_:rdf3251","@type":["http:\/\/ocean-data.org\/schema\/MetadataDownloadAffordance"],"http:\/\/schema.org\/subjectOf":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset\/3251"}],"http:\/\/schema.org\/name":[{"@value":"RDF\/XML","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/affordedBy":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/affiliation\/191"}],"http:\/\/schema.org\/target":[{"@value":"_:rdf3251entryPoint"}]},"_:rdf3251entryPoint":{"@id":"_:rdf3251entryPoint","@type":["http:\/\/schema.org\/EntryPoint"],"http:\/\/schema.org\/url":[{"@value":"https:\/\/www.bco-dmo.org\/dataset\/3251.rdf","@type":"xsd:anyURI"}],"http:\/\/schema.org\/contentType":[{"@value":"application\/rdf+xml","@type":"xsd:token"}]},"_:iso3251":{"@id":"_:iso3251","@type":["http:\/\/ocean-data.org\/schema\/MetadataDownloadAffordance"],"http:\/\/schema.org\/subjectOf":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset\/3251"}],"http:\/\/schema.org\/name":[{"@value":"ISO 19115-2 (NOAA Profile)","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/affordedBy":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/affiliation\/191"}],"http:\/\/schema.org\/target":[{"@value":"_:iso3251entryPoint"}]},"_:iso3251entryPoint":{"@id":"_:iso3251entryPoint","@type":["http:\/\/schema.org\/EntryPoint","http:\/\/ocean-data.org\/schema\/ISOMetadata"],"http:\/\/schema.org\/url":[{"@value":"https:\/\/www.bco-dmo.org\/dataset\/3251\/iso","@type":"xsd:anyURI"}],"http:\/\/schema.org\/contentType":[{"@value":"application\/xml","@type":"xsd:token"}],"http:\/\/purl.org\/dc\/terms\/conformsTo":[{"@value":"http:\/\/www.isotc211.org\/2005\/gmd-noaa","@type":"xsd:anyURI"}]},"_:dublincore3251":{"@id":"_:dublincore3251","@type":["http:\/\/ocean-data.org\/schema\/MetadataDownloadAffordance"],"http:\/\/schema.org\/subjectOf":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/dataset\/3251"}],"http:\/\/schema.org\/name":[{"@value":"Dublin Core","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/affordedBy":[{"@id":"http:\/\/lod.bco-dmo.org\/id\/affiliation\/191"}],"http:\/\/schema.org\/target":[{"@value":"_:dublincore3251entryPoint"}]},"_:dublincore3251entryPoint":{"@id":"_:dublincore3251entryPoint","@type":["http:\/\/schema.org\/EntryPoint"],"http:\/\/schema.org\/url":[{"@value":"https:\/\/www.bco-dmo.org\/dataset\/3251\/dublin-core","@type":"xsd:anyURI"}],"http:\/\/schema.org\/contentType":[{"@value":"application\/xml","@type":"xsd:token"}],"http:\/\/purl.org\/dc\/terms\/conformsTo":[{"@value":"http:\/\/purl.org\/dc\/elements\/1.1\/","@type":"xsd:anyURI"}]},"_:Identifier3251":{"@id":"_:Identifier3251","@type":["http:\/\/ocean-data.org\/schema\/Identifier","http:\/\/ocean-data.org\/schema\/BCODMOIdentifier","http:\/\/ocean-data.org\/schema\/OSPREY_v2_Node_dataset"],"http:\/\/ocean-data.org\/schema\/identifierScheme":[{"@id":"http:\/\/ocean-data.org\/schema\/IdentifierScheme_BCODMO_Version_2"}],"http:\/\/ocean-data.org\/schema\/identifierValue":[{"@value":"3251","@type":"xsd:token"}],"http:\/\/ocean-data.org\/schema\/resolvableURL":[{"@value":"http:\/\/lod.bco-dmo.org\/id\/dataset\/3251","@type":"xsd:anyURI"}]},"_:spatialCoverage3251":{"@id":"_:spatialCoverage3251","@type":["http:\/\/www.opengis.net\/ont\/sf#Polygon"],"http:\/\/www.opengis.net\/ont\/geosparql#asWKT":[{"@value":"