Dataset: Series 1: Abiotic TEP Formation - DIC Perturbation

Name: The effect of altered carbonate system chemistry on the abiotic formation of Transparent Exopolymer Particles (TEP) from UCSB MSI Passow Laboratory from 2009 to 2010 (OA - Ocean Acidification and Aggregation project)
Published: 2012-03-20
Keywords: oceans

Cite This Dataset

Spatial Extent: N:34.4126 E:-119.842 S:34.4126 W:-119.842

Project:

Will Ocean Acidification Diminish Particle Aggregation and Mineral Scavenging, Thus Weakening the Biological Pump? (OA - Ocean Acidification and Aggregation)

Program:

Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA) (SEES-OA)

Ocean Carbon and Biogeochemistry (OCB)

Principal Investigator:

Uta Passow (University of California-Santa Barbara, UCSB-MSI)

Co-Principal Investigator:

Dr Alice L. Alldredge (University of California-Santa Barbara, UCSB-MSI)

Contact:

Uta Passow (University of California-Santa Barbara, UCSB-MSI)

BCO-DMO Data Manager:

Stephen R. Gegg (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

20 March 2012

Version Date:

2012-03-20

Validated:

Current State:

Final no updates expected

The effect of altered carbonate system chemistry on the abiotic formation of Transparent Exopolymer Particles (TEP) from UCSB MSI Passow Laboratory from 2009 to 2010 (OA - Ocean Acidification and Aggregation project)

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Description

Series 1: Abiotic TEP Formation - DIC Perturbation

Results form a series of controlled laboratory experiments investigating the effect of altered carbonate system chemistry on the abiotic formation of Transparent Exopolymer Particles (TEP).

The over determination columns (pCO2_pH_TA, pCO2_DIC_pH, pCO2_DIC_TA) give pCO2 (calculated using CO2Sys) for samples for which we measured three parameters, pH, total alkalinity (TA) and dissolved inorganic carbon (DIC). In all others we only measured pH and TA. These columns each give the calculated pCO2 in uatm, using pH and TA; DIC and pH and the DIC and TA respectively for calculations.

Methods & Sampling

See: Passow U (2012) The Abiotic Formation of TEP under Ocean Acidification Scenarios. Marine Chemistry 128-129:72-80

Data Processing Description

See: Passow U (2012) The Abiotic Formation of TEP under Ocean Acidification Scenarios. Marine Chemistry 128-129:72-80

Co2Sys was used to calculate the carbonate system parameter pCO2.

pCO2 calculated from total alkalinity, DIC and pH (total scale) using Co2Sys

BCO-DMO Processing Notes
Original files: "Data.xls, Sheet: DICperturbation" and "Overcompensation.xls" contributed by Uta Passow
- Approx Lat/Lon of Passow Lab appended to enable data discovery in MapServer
- "nd" (no data) inserted into blank cells
- Parameter names edited to conform to BCO-DMO parameter naming conventions

Data Files

File
Series1_AbioticTEP_DICpert.csv (Comma Separated Values (.csv), 7.23 KB) MD5:6579273c69d682a2c17182583c575785 Primary data file for dataset ID 3623

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-0926711

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
lab_UCSB_MSI_Passow	Passow Lab	2009-09-01	UCSB MSI Passow	Uta Passow (Principal Investigator)	data info

Instruments

Automatic titrator

Supplied Name: Automatic titrator - T50, Mettler Toledo

Supplied Description:

In brief, TA (μmol kg− 1) was measured within 4 hours after sampling by potentiometric titration (T50, Mettler Toledo) using certified acid titrant.

Instrument Type

Generic Name: Automatic titrator

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/

Generic Description:

Instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached.

Bottle

Supplied Name: bottle

Supplied Description:

Experiments 3 and 4, respectively, were conducted with 10 μm pre-filtered natural seawater and 10 μm pre-filtered sea water first incubated in 20 L bottles (bottle blooms).

Instrument Type

Generic Name: Bottle

Generic Description:

A container, typically made of glass or plastic and with a narrow neck, used for storing drinks or other liquids.

Conductivity Meter

Supplied Name: Conductivity Meter - 3100 Yellow Springs Instruments

Supplied Description:

Salinity was determined from TA samples using a conductivity instrument (3100 Yellow Springs Instruments).

Instrument Type

Generic Name: Conductivity Meter

Generic Description:

Conductivity Meter - An electrical conductivity meter (EC meter) measures the electrical conductivity in a solution. Commonly used in hydroponics, aquaculture and freshwater systems to monitor the amount of nutrients, salts or impurities in the water.

In-situ incubator

Supplied Name: in-situ incubator

Supplied Description:

Instrument Type

Generic Name: In-situ incubator

Acronym: in-situ incubator

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/82/

Generic Description:

A device on a ship or in the laboratory that holds water samples under controlled conditions of temperature and possibly illumination.

Pump

Supplied Name: Aquarium Pump

Supplied Description:

Bottle blooms were incubated in clear 20 L bottles at 14 °C, at 15–30 μE s − 1 for 12:12 hours and bubbled with an aquarium pump during the light hours to keep the carbonate system stable.

Instrument Type

Generic Name: Pump

Generic Description:

A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps

Roller Tank

Supplied Description:

All experiments were conducted in rolling tanks at 14 °C in the dark.

Instrument Type

Generic Name: Roller Tank

Generic Description:

Rolling tanks, which keep particles in suspension, thus simulating aggregate formation in situ.

Marine snow experiments are conducted in roller tanks, which turn continuously, keeping marine snow in suspension. It is important for marine snow not to touch surfaces. The rolling tanks, which keep particles in suspension, thus simulate aggregate formation in situ. Marine snow formation due to different types of oil was tested. Some treatments are easily identifiable as containing oil by their color (middle). UCSB, CA 2012.

Spectrophotometer

Supplied Name: spectrophotometer - Shimadzu Biospec 16-1

Supplied Description:

The pHT (total scale) was measured with a spectrophotometer (Shimadzu Biospec 16-1) using the indicator dye mcresol purple (Sigma-Aldrich) within 1–2 hours after sampling.

Instrument Type

Generic Name: Spectrophotometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/

Generic Description:

An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples.

Turner Designs 700 Laboratory Fluorometer

Supplied Name: TD-700

Supplied Description:

Chl. a was determined during growth of cultures and bottle blooms using a fluorometer (Turner 700) by filtration of samples onto 0.4 μm PC filters and soaking the filters in 90% acetone over night in the freezer (Strickland, 1972).

Instrument Type

Generic Name: Turner Designs 700 Laboratory Fluorometer

Acronym: TD-700

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0510/

Generic Description:

The TD-700 Laboratory Fluorometer is a benchtop fluorometer designed to detect fluorescence over the UV to red range. The instrument can measure concentrations of a variety of compounds, including chlorophyll-a and fluorescent dyes, and is thus suitable for a range of applications, including chlorophyll, water quality monitoring and fluorescent tracer studies. Data can be output as concentrations or raw fluorescence measurements.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Lab_Id	Lab Id – Lab identifier where experiments were conducted	text	laboratory
Lat	Approximate Latitude Position of Lab; South is negative	decimal degrees	lat
Lon	Approximate Longitude Position of Lab; West is negative	decimal degrees	lon
Experiment_Number	Experiment Number	integer	exp_id
Date_Range	Experiment Date Range	MM/DD/YR – MM/DD/YR	date_range
Substrate	Substrate	text	no_bcodmo_term
Treatment	Treatments	text	treatment
Replicates	Replicates	text	replicate
pCO2_T0_pH_TA	pCO2 at T0	micro atmospheres	pCO2
pCO2_T24_pH_TA	pCO2 at T24	micro atmospheres	pCO2
pCO2_pH_TA	pCO2 pH TA	micro atmospheres	pCO2
pCO2_DIC_pH	pCO2 DIC pH	micro atmospheres	pCO2
pCO2_DIC_TA	pCO2 DIC TA	micro atmospheres	pCO2
pH_T0	pH at T0	sea water scale	pH
pH_T24	pH at T24	sea water scale	pH
TEP_T0	TEP at T0 in ug of Gxeq (xanthan gum weight equivalent) per liter	ug Gxeq/L	TEP
TEP_T24	TEP at T24 in ug of Gxeq (xanthan gum weight equivalent) per liter	ug Gxeq/L	TEP

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Dataset: Series 1: Abiotic TEP Formation - DIC Perturbation