Dataset: recruit_growth_weight

Name: Coral recruit growth measured by weight change from calcification during CO2 experiments collected from the Natl Museum Mar. Bio. and Aquar., Taiwan in 2010 (MCR LTER project, Climate_Coral_Larvae project)
Published: 2014-04-09
Keywords: oceans

Cite This Dataset

Spatial Extent: N:22.0497 E:120.6985 S:22.0497 W:120.6985

Project:

Moorea Coral Reef Long-Term Ecological Research site (MCR LTER)

The ecophysiological basis of the response of coral larvae and early life history stages to global climate change (Climate_Coral_Larvae)

Program:

Long Term Ecological Research network (LTER)

Lead Principal Investigator:

Peter J. Edmunds (California State University Northridge, CSUN)

Student:

Aaron M. Dufault (California State University Northridge, CSUN)

BCO-DMO Data Manager:

Danie Kinkade (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

2014-03-07

Version Date:

2014-04-09

Restricted:

Validated:

Current State:

Preliminary and in progress

Coral recruit growth measured by weight change from calcification during CO2 experiments collected from the Natl Museum Mar. Bio. and Aquar., Taiwan in 2010 (MCR LTER project, Climate_Coral_Larvae project)

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Description

Manipulative studies have demonstrated that ocean acidification (OA) is a threat to coral reefs, yet no experiments have employed diurnal variations in pCO2 that are ecologically relevant to many shallow reefs. Two experiments were conducted to test the response of coral recruits (less than 6 days old) to diurnally oscillating pCO2; one exposing recruits for 3 days to ambient (440 uatm), high (663 uatm) and diurnally oscillating pCO2 on a natural phase (420–596 uatm), and another exposing recruits for 6 days to ambient (456 uatm), high (837 uatm) and diurnally oscillating pCO2 on either a natural or a reverse phase (448–845 uatm).

These data are published in Dufault et al. (2012), Proc. R. Soc. B. doi:10.1098/rspb.2011.2545

Related Datasets:

recruit_seawater_chemistry
recruit_growth_area
recruit_suvival

Methods & Sampling

Larvae were obtained from brooding colonies coral S. caliendrum collected from 5 to 7 m deep on Hobihu Reef, Nanwan Bay, in March and June of 2010, placed into individual flow-through seawater tanks. Overflow water from each tank passed through mesh-lined (110 mm) cups that captured larvae. Following collection, larvae were settled onto clean pre-weighed glass microscope coverslips. Coverslips with coral recruits (n=18: experiment I; n=36: experiment II) were assigned randomly to the pCO2 treatments.

In experiment I, treatments consisted of steady ambient pCO2, steady high pCO2 and diurnally oscillating pCO2 on a natural phase; this design was augmented in experiment II by including a diurnally oscillating pCO2 on a reverse phase.

Upon completion of the experiments, coverslips with coral recruits were placed in bleach (6% NaOCl) for 8 h to dissolve the tissue on the small corals and leave behind the CaCO3 skeleton. Coverslips were then rinsed with deionized water to remove the bleach and air-dried for 24 h at approximately 278C. Calcification was measured using the summed weight of the CaCO3 deposited by recruits on each coverslip and also as the planar area of the basal plate of each recruit. Coverslips without recruits but subjected to identical treatments served as procedural controls, and these did not change in weight in either experiment. In experiment I, the change in weight of each coverslip was divided by the number of corallites to provide a mean weight that was used as a statistical replicate. As some (approx. 5%) recruits died during the experiment, this technique slightly underestimated calcification.To remove this bias in experiment II, only recruits alive at the end of the experiment were used for growth measurements.

For detailed description of methods, see Dufault et al. (2012), Proc. R. Soc. B. doi:10.1098/rspb.2011.2545

Data Processing Description

For detailed description of processing see Dufault et al. (2012), Proc. R. Soc. B. doi:10.1098/rspb.2011.2545

Data Files

File
CoralRecruit_growth_weight.csv (Comma Separated Values (.csv), 5.29 KB) MD5:29ae36ffb1d84f40108d9d31dd8f82ec Primary data file for dataset ID 506245

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-0844785

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
lab_Edmunds_NMMBA		2010-03-18	Natl Museum Mar. Bio. and Aquar. Taiwan	Peter J. Edmunds (Lead Principal Investigator)	data info

Instruments

Gas Analyzer

Supplied Name: infrared gas analyzer

Supplied Description:

A gas analyzer S151, Qubit Systems (Ontario Canada) was used during this experiment.

Instrument Type

Generic Name: Gas Analyzer

Generic Description:

Gas Analyzers - Instruments for determining the qualitative and quantitative composition of gas mixtures.

Microscope - Optical

Supplied Name: compound microscope

Supplied Description:

A Zeiss Axiolab E compound microscope with 40X magnification was used during this experiment.

Instrument Type

Generic Name: Microscope - Optical

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/

Generic Description:

Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
laboratory	Laboratory where experiments were conducted.	unitless	laboratory
experiment	Experiment identifier: Experiment I treatments consisted of steady ambient pCO2, steady high pCO2 and diurnally oscillating pCO2 on a natural phase. Experiment II treatments consisted of a diurnally oscillating pCO2 on a reverse phase.	dimensionless	exp_id
lat	Latitude component of geographic position where experiments were conducted.	decimal degrees	lat
lon	Longitude component of geographic position where experiments were conducted.	decimal degrees	lon
duration	Experiment duration: 3 (Exp I) or 6 days (Exp. II).	days	time_inc
coverslip	Unique coverslip the coral was settled upon.	dim	sample
number_polyps	Number of polyps at the end of the experiment on the coverslip.	dimensionless	number
treatment_CO2	Individual CO2 treatment (A=ambient, H=High, D=Diurnal, DX=Reverse-phase Diurnal). Originally reported as "CO2".	dimensionless	treatment
tank	Unique tank the coverslips with settled corals were placed in (a decimal number represents 2 tanks used for the diurnal treatments - corals were placed in the first tank during the day (1st number) and into the second tank at night (2nd number), eg Tank 1.8= tank 1 during day, tank 8 at night).	dimensionless	tank
month	Month of coral colony collection.	dimensionless	month
year	Year of coral colonly collection.		year
growth	The total change in weight (mg) formed by the deposition of CaCO3 by the growing corals.	milligrams	growth
growth_polyp	The total weight of deposited CaCO3 (mg) normalized to the number of polyps.	milligrams polyp-1	growth
growth_polyp_day	The growth per polyp (mg) normalized to the duration of the study.	milligrams polyp-1 day-1	growth
transformation	The 4th root transformation of data to address positive skewing in Experiment I.	unknown	no_bcodmo_term

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Dataset: recruit_growth_weight