10.1111\/gcb.12658<\/a><\/p>\nA brief description of the analytical methods follows.<\/p>\n
Calcification, endosymbiotic algae concentration, total energy reserves, and Symbiodinium identification<\/em><\/strong>.\u00a0 Calcification rates were calculated from the buoyant weight data (Jokiel et al.<\/em>, 1978) and standardized to surface area.\u00a0 Endosymbiont cell concentration (Warner et al.<\/em>, 2006), and total soluble lipid, soluble animal protein, and soluble animal carbohydrates (Levas et al.<\/em>, 2013, Rodrigues &\u00a0 Grottoli, 2007) were measured on all frozen fragments. Total energy reserves were calculated as the sum of total lipids, protein, and carbohydrates and reported in Joules (Gnaiger &\u00a0 Bitterlich, 1984) per gram ash free dry weight of coral tissue. Since polyp structure and the coral tissue thickness of each species are different, this normalization facilitates inter-species comparisons (Edmunds &\u00a0 Gates, 2002). Genetic characterizations of Symbiodinium<\/em> were determined by amplification of the internal-transcribed spacer 2 region (ITS2), followed by denaturing gradient gel electrophoresis and cycle-sequencing (Warner et al.<\/em>, 2006). This method reliably identifies the dominant symbiont type in healthy and bleached corals (LaJeunesse et al.<\/em>, 2004, LaJeunesse et al.<\/em>, 2009, Warner et al.<\/em>, 2006) and provides qualitative identification of other background Symbiodinium<\/em>, either within different clades (e.g., endosymbionts A3 vs B1) or at the intra-cladal scale (e.g., endosymbionts A3 and A13) within the same coral fragment.\u00a0 The dominant ITS2-types (intra-cladal designations) are listed for each coral species in the text, while for statistical analyses (described below), the dominant symbiont for each coral fragment treatment-1 was grouped by clade. Specific quantitative PCR for all clades confirmed the accuracy of scoring dominant bands by DGGE analysis (data not shown, McGinley, 2012).<\/p>\nPhotosynthesis, respiration, and feeding<\/em>.<\/strong>\u00a0 Maximal photosynthesis and respiration rates were measured via changes in dissolved oxygen on each individual coral fragment immediately following their respective thermal stress then standardized to ash free dry weight (Rodrigues &\u00a0 Grottoli, 2007). All fragments were placed back on the reef and then feeding rates of each coral fragment were determined using methods in Palardy et al. (2008). Photosynthesis and respiration were used to calculate the percent Contribution of Zooxanthellae (Symbiodinium <\/em>spp.) to Animal Respiration (CZAR) (Muscatine et al.<\/em>, 1981), while respiration and feeding rates were used to calculate the percent Contribution of Heterotrophy to Animal Respiration CHAR (Grottoli et al.<\/em>, 2006, Palardy et al.<\/em>, 2008). The Contribution of the Total acquired fixed carbon relative to Animal Respiration (CTAR) was calculated as the sum of CZAR and CHAR.<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/ocean-data.org\/schema\/hasBriefDescription":[{"@value":"Coral metabolism, energy reserves, calcification, algal density and type","@language":"en-US"}],"http:\/\/purl.org\/dc\/terms\/description":[{"@value":"Coral metabolism, energy reserves, calcification, algal density and type.<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/www.w3.org\/2000\/01\/rdf-schema#label":[{"@value":"coral physiology and algal type","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/hasProcessingDescription":[{"@value":"
Three statistical outliers were removed in order to meet assumptions of normality and homogeneity prior to ANOVA analyses.\u00a0 These data are flagged in the excel file and the removed value is included as a comment.\u00a0 Details of the statistical analysis methods are in Grottoli et al.\u00a0<\/em>(in press).<\/p>\nBCO-DMO Data Processing:
\n- Added BCO-DMO header info to include brf dscrptn, PI and version
\n- Edited parameter headers to comply with BCO-DMO convention:<\/p>\n
- Total EnRes\u00a0-->\u00a0energy_rsrv_total<\/li>\n
- 'number endosymbiont cells\/cm2' --> count_endosymbiont<\/li>\n
- 1o Symbiodinium type --> symbiodinium_primary_type<\/li>\n
- 2o Symbiodinium type --> symbiodinium_secondary_type<\/li>\n
- 3o Symbiodinium type --> symbiodinium_tertiary_type<\/li>\n
- year\u00a0-->\u00a0treatment_year<\/li>\n
- time --> treatment_recovery_time<\/li>\n<\/ul>
- Species was decoded and genus species names were included in data, replacing\u00a0codes of 1,2,3
\n- Replaced all \".\" with \"nd\"
\n- Added lat\/lon of the collection site of coral fragments (Puerto Morelos 20.8333, 86.8666)
\n- All precisions were edited to two decimals after consultation with PI.
\n- Corrected longitude values to negative degrees. 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