Dataset: algae_calcification

Name: Experimental results of calcification rates of algae at variable pCO2 concentrations from the Richard B Gump Research Station, Moorea, French Polynesia in 2011 (MCR LTER project, OA_Corals project)
Published: 2015-03-21
Keywords: oceans

Cite This Dataset

Spatial Extent: N:-17.4907 E:-149.826 S:-17.4907 W:-149.826

Project:

Moorea Coral Reef Long-Term Ecological Research site (MCR LTER)

RUI: Ocean Acidification- Category 1- The effects of ocean acidification on the organismic biology and community ecology of corals, calcified algae, and coral reefs (OA_Corals)

Program:

Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA) (SEES-OA)

Long Term Ecological Research network (LTER)

Lead Principal Investigator:

Steeve Comeau (California State University Northridge, CSUN)

Co-Principal Investigator:

Robert Carpenter (California State University Northridge, CSUN)

Peter J. Edmunds (California State University Northridge, CSUN)

BCO-DMO Data Manager:

Danie Kinkade (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version Date:

2015-03-21

Restricted:

Current State:

Preliminary and in progress

Experimental results of calcification rates of algae at variable pCO2 concentrations from the Richard B Gump Research Station, Moorea, French Polynesia in 2011 (MCR LTER project, OA_Corals project)

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Description

These data were obtained during an experiment performed in August-September-October 2011 and 2012 in French Polynesia. Calcification rates were measured in 8 algae (Porolithon onkodes, Lithophyllum flavescens, Halimeda macroloba, Halimeda minima, Hydrolithon reinboldii, Lithophyllum kotschyanum, Amphiroa fragilissima, Neogoniolithon frutescens) at 6 pCO2.

Related References:

Comeau, S., Edmunds, P. J., Spindel, N. B., Carpenter, R. C. (2014). Fast coral reef calcifiers are more sensitive to ocean acidification in short-term laboratory incubations. Limnology and Oceanography, 59(3), 1081–1091. doi:10.4319/lo.2014.59.3.1081

Comeau, S., Edmunds, P. J., Spindel, N. B., Carpenter, R. C. (2013) The responses of eight coral reef calcifiers to increasing partial pressure of CO2 do not exhibit a tipping point. Limnol. Oceanogr. 58, 388–398.

Methods & Sampling

For the carbonate chemistry:

Seawater pH was measured twice a day (08:00 hrs and 18:00 hrs) in each incubation tank, using a pH meter (Orion, 3-stars mobile) calibrated every 2-3 d on the total scale using Tris/HCl buffers (Dickson, San Diego, USA) with a salinity of 34.5. Total alkalinity of the seawater in the tanks was measured every 2 d, using single samples drawn from each tank in glass-stoppered bottles (250 mL). Samples were analyzed for total alkalinity within 1 d using open cell, potentiometric titration and an automatic titrator (T50, Mettler-Toledo). Titrations were conducted on 50-mL samples at ~ 24 °C, and total alkalinity calculated as described by Dickson et al. [2007]. Titrations of certified reference materials (CRM) provided by A. G. Dickson (batch 105) yielded total alkalinity values within 3.8 umol kg-1 of the nominal value (SD = 3.1 umol kg-1; n = 19). Salinity in the experimental tanks was measured every 2 d using a conductivity meter (YSI 3100). Total alkalinity, pH on the total scale (pHT), temperature, and salinity were used to calculate the carbonate chemsitry using the seacarb package (Lavigne and Gattuso 2012) running in R software (R Foundation for Statistical Computing).

Calcification:

Buoyant weight (± 1 mg) was recorded before and after the 15 d of incubation (following 15 d of acclimation), and the difference between the two was converted to dry weight using an aragonite density of 2.93 g cm^-3 for Halimeda, and a calcite density of 2.71 g cm^-3 for coralline algae. Calcification was normalized by biomass for algae.

Data Processing Description

Parameters of the carbonate system in seawater were calculated from salinity, temperature, A_T and pH_T using the R package seacarb (Lavigne and Gattuso 2011).

Data Files

File
algae_calc.csv (Comma Separated Values (.csv), 44.35 KB) MD5:33c92e914e0bdfbe900e15880e9419b9 Primary data file for dataset ID 518491

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1041270

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
lab_Carpenter_Moorea		2011-07-17	Richard B Gump Research Station - Moorea LTER	Peter J. Edmunds (Chief Scientist) Robert Carpenter (Co-Chief Scientist) Steeve Comeau (Contact)	data info

Instruments

Automatic titrator

Supplied Description:

Instrument Type

Generic Name: Automatic titrator

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/

Generic Description:

Instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached.

pH Sensor

Supplied Description:

Instrument Type

Generic Name: pH Sensor

Generic Description:

An instrument that measures the hydrogen ion activity in solutions.

The overall concentration of hydrogen ions is inversely related to its pH. The pH scale ranges from 0 to 14 and indicates whether acidic (more H+) or basic (less H+).

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
species_alga	Algal species used during experiment.	dimensionless	species
treatment_pCO2	pCO2 concentration for each species treatment.	microatmospheres	treatment
rate_calcification	Calcification of algae normalized by biomass (tissue dry weight).	mg CaCO3 g-1 DW d-1	no_bcodmo_term
lab	Laboratory where experiments were conducted.		laboratory
lat	Latitude component of geographic location.	decimal degrees	lat
lon	Longitude component of geographic location.	decimal degrees	lon

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Dataset: algae_calcification