Dataset: Grazing experiment 2 and 3: cell size

Name: Cell size measurements of low-high pCO2 acclimated E. huxleyi (E Hux Response to pCO2 project)
Published: 2016-10-11
Keywords: biota, oceans

Cite This Dataset

Project:

Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton (E Hux Response to pCO2)

Principal Investigator:

M Brady Olson (Western Washington University, WWU)

Co-Principal Investigator:

Brooke Love (Western Washington University, WWU)

Suzanne Strom (Western Washington University, WWU)

Student:

Kasey Kendall (Western Washington University, WWU)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version Date:

2016-10-11

Restricted:

Release Date:

2017-12-31

Validated:

Yes

Current State:

Final no updates expected

Cell size measurements of low-high pCO2 acclimated E. huxleyi (E Hux Response to pCO2 project)

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Description

These data are unprocessed Emiliania huxleyi cell volumes.

Related Reference:
Kendall, K., Marine Microzooplankton are Indirectly Affected by Ocean Acidification Through Direct Effects on Their Phytoplankton Prey. (Masters Thesis) Western Washington University.
http://cedar.wwu.edu/wwuet/448/

Methods & Sampling

The phytoplankton Emiliania huxleyi CCMP 2668 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv). Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients.

Expt. 2: On day 8, after ~16 generations, E. huxleyi cells from the treatments were mounted live on a microscope slide and 200 cells from each treatment were imaged using RSImage software under 400X magnification on an Olympus CHA microscope.

Expt. 3: On day 10, after ~ 20 generations, E. huxleyi cells from the treatments were mounted live on a microscope slide and 195-204 cells from each treatment were imaged using RSImage software under 400X magnification on an Olympus CHA microscope.

Since E. huxleyi cells are rough spheres, the volume of the spherical was calculated using: V(µm³) = 4/3 πr³. The radius was calculated as: r(µm)=square root of (A/π). The area, A, was determined from the 2-D images using ImageJ software.

Data Processing Description

BCO-DMO Processing:
- added conventional header with dataset name, PI name, version date
- column names reformatted to comply with BCO-DMO standards
- added column 'expt' for experiment number
- experiments 2 and 3 data were concatenated into one data set

Data Files

File
expt2_3_cell_vol.csv (Comma Separated Values (.csv), 39.73 KB) MD5:659408c55c934540fc7bee69479fd8b6 Primary data file for dataset ID 661464

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-0961229

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
Lab_Olson_B		2011-03-31	WWU		data info

Instruments

Microscope - Optical

Supplied Name:

Supplied Description:

Olympus CHA microscope

Instrument Type

Generic Name: Microscope - Optical

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/

Generic Description:

Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
expt	experiment identification	unitless	exp_id
treatment_cell_number	pCO2 level (ambient; moderate; high) and cell id number	unitless	treatment
cell_area_um	area of the cell	micrometers^2	no_bcodmo_term
cell_radius_um	radius of the cell	micrometers	no_bcodmo_term
cell_volume_um	volume of the cell	micrometers^3	volume

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Database

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Dataset: Grazing experiment 2 and 3: cell size