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1. Sampling:<\/strong><\/p>\n Size-fractionated particles were collected using McLane Research in-situ pumps (WTS-LV) that had been modified to accommodate two flowpaths (Ohnemus and Lam, 2015). \u00a0Typically, two casts of 8 pumps each and two filter holders per pump were deployed to collect a 16-depth profile. \u00a0At superstations, three casts were deployed to collect a 24-depth profile. \u00a0The wire-out was used to target nominal depths during deployment. A self-recording Seabird 19plus CTD was deployed at the end of the line and used to verify target depths.\u00a0<\/p>\n Filter holders used were 142 mm-diameter \u201cmini-MULVFS\u201d style filter holders with two stages for two size fractions and multiple baffle systems designed to ensure even particle distribution and prevent particle loss (Bishop et al., 2012). \u00a0One filter holder\/flowpath was loaded with a 51um Sefar polyester mesh prefilter followed by paired Whatman QMA quartz fiber filters. The other filter holder\/flowpath was also loaded with a 51um prefilter, but followed by paired 0.8um Pall Supor800 polyethersulfone filters. All filters and filter holders were acid leached prior to use according to methods recommended in the GEOTRACES sample and sample-handing Protocols (GEOTRACES, 2010). \u00a0QMA filters were additionally precombusted at 450 deg C for four hours.<\/p>\n Each cast also had a full set of \u201cdipped blank\u201d filters deployed. \u00a0In the first half of the cruise, these were the full filters sets (51 um prefilter followed by paired QMA or paired Supor filters) loaded in mini-MULVFS filter holders that were mounted on a pump with a specially designed dipped blank plate to accommodate two extra filter holders not connected to the pump. \u00a0A 0.2 um Supor prefilter above the filter set excluded any particles that may have been present in seawater in the filter holder headspace. \u00a0At station 21, the specially designed plate (along with the filter holders) was lost at sea when the ship lost power, so subsequent dipped blank filters were the full filter sets sandwiched within a 1um polyester mesh filter, loaded into acid-leached perforated polypropylene containers, and attached with plastic cable ties to a pump frame, and deployed. \u00a0All dipped blank filters were exposed to seawater for the length of the deployment and processed and analyzed as regular samples, and thus functioned as full seawater process blanks. \u00a0A total of 58 dipped blank filter sets were collected and used for blank subtraction and determination of uncertainty and detection limit (Table 1).<\/p>\n In this dataset, data reported from the 51um prefilter are referred to with a LPT suffix to indicate large particulate total concentrations (>51um); data reported from the main filters (QMA\u20141-51um\u2014or Supor\u20140.8 um-51um) are from the top filter of the pair only, and are referred to with a SPT suffix to indicate the small particulate total concentrations.<\/p>\n 2. Analytical Methodology and Data Processing:\u00a0<\/strong><\/p>\n For all parameters described below, the median dipped blank value (Table 1) was subtracted. \u00a0For most parameters, we could not routinely run replicates, so the reported error is determined from the standard deviation of dipped blank filters (Table 1), converted to concentrations using volume filtered. \u00a0This assumes that the blank subtraction is the largest source of error. \u00a0The detection limit was defined as three times the standard deviation of the dipped blank filters. \u00a0Values below the detection limit were flagged as QF=4. The percentage of all samples that fell below the detection limit is listed in Table 1 for each parameter and size fraction. \u00a0Any variations on the methods for blank subtraction or determination of error are further described for each parameter, as necessary.<\/p>\n 2.1. Biogenic Silica and Opal<\/p>\n 2.1.1 Analytical methodology for biogenic silica<\/p>\n A 1\/16 subsample of the top 0.8um Supor filter, equivalent to ~30L, or of the 51um polyester prefilter above the QMA filter, equivalent to ~60L, was analyzed for amorphous\/biogenic Si concentrations using spectrophotometric detection of the blue silico-molydate complex following an alkaline (0.2M NaOH) leach at 85 deg C. \u00a0Some initial stations were run manually on a Shimadzu UV-2550 UV-VIS Spectrophotometer at WHOI, but most samples were run on a Lachat QuikChem 8000 Flow Injection Analyzer at UCSC. \u00a0The principle of both methods is the same, except that formation of the silico-molybdate complex reaction is allowed to run to completion before spectrophotometric detection in the manual method (WHOI), whereas the reaction occurs online in the flow injection method (UCSC).<\/p>\n A time-series approach was tested for the WHOI leaches, with four timepoints taken after 1, 2, 3, and 4 hours of leaching. \u00a0There was little evidence of a systematic lithogenic contribution, so the mean of the first three timepoints from the WHOI leaches was taken as the most representative value, and the standard deviation used in the error estimate. \u00a0Subsequent samples at UCSC were leached for 1 hr only.<\/p>\n 2.1.2 Data Processing<\/p>\n Intercomparability of the WHOI and UCSC methods was assessed by checking that the bSi profiles of stations with samples analyzed at both places were oceanographically consistent.<\/p>\n The median of Supor dipped blank filters were used to correct the SPT data. \u00a0For >51 um LPT samples on polyester prefilters, blank corrections were made using the median failed pump values (pumps that never turned on, or that shut off after <5% of programmed water volume was filtered) because of anomalously high prefilter dipped blank values. Anomalously high prefilter dipped blank bSi values was also observed for the GA03 NAZT section. \u00a0<\/p>\n The reported error was determined as the standard deviation of bSi on the dipped blank filters, converted to concentrations using volume filtered. For samples run at WHOI only, the standard deviation of the three timepoints was also propagated into the error estimate.<\/p>\n 2.1.3 Derived parameters: OPAL<\/p>\n The mass of opal was calculated assuming a hydrated form of silica: SiO2.(0.4 H2O) (Mortlock and Froelich, 1989), or 67.2 g opal\/mol bSi:<\/p>\n OPAL [ug\/L] = bSi [uM] *67.2 [ug opal\/umol bSi]<\/p>\n 2.2 Particulate Organic Carbon (POC), Particulate Nitrogen (PN), POM, \u03b413C, and d15N<\/p>\n 2.2.1 Analytical methodology for POC, PN, \u03b413C, and d15N<\/p>\n Particulate organic carbon and particulate nitrogen were measured by combustion on an elemental analyzer after fuming with concentrated HCl to remove particulate inorganic carbon. \u00a0Briefly, dried filter samples were exposed to HCl fumes in a dessicator for ~16 hours. After fuming, filters were dried at 60 deg C in an oven for another ~16 hrs before being pelletized in tin discs. POC from suspended particles (1-51um; SPT) were measured using a Flash EA1112 Carbon\/Nitrogen Analyzer using a Dynamic Flash Combustion technique at the WHOI Nutrient Analytical Facility on two 12 mm-diameter punches from the top QMA filter, representing the equivalent of ~20L of material. For the >51um size fraction (LPT), particles from the 51um polyester prefilter above the Supor filter were rinsed at sea with 1um-filtered seawater onto a 25mm 1.2 um Sterlitech Ag filter and dried at 60 deg C. \u00a0Half of the Ag filter containing rinsed LPT particles, typically representing ~200L of material, was analyzed for POC and PN using either a Carlo Erba 1108 or a CE Instruments NC2500 elemental analyzer interfaced to a ThermoFinnigan Delta Plus XP isotope ratio mass spectrometer at the Stable Isotope Laboratory at UCSC, which also gave the C and N stable isotope composition of the LPT particles.<\/p>\n 2.2.2 Data Processing<\/p>\n Intercomparability of the WHOI and UCSC datasets was assessed by checking that a POC_SPT profile that had samples analyzed at both places was oceanographically consistent.<\/p>\n For POC and PN in the small size fraction, the standard deviation of 47 dipped blank QMA filters used for blank subtraction. \u00a0For POC and PN in the large size fractions \u00a0(>51 um; POC_LPT, PN_LPT), we found that the variability in the dipped blank increased significantly after station 21 (i.e., after the loss of the dipped blank plate and switching to the perforated plastic holder for the dipped blanks), especially for the deeper casts. \u00a0We therefore use the median and standard deviation of 25 dipped blanks from before station 21 for blank subtraction and error estimation (Table 1).\u00a0<\/p>\n The stable isotopic composition of C (\u03b413C) and N (d15N ) for the large size fraction LPT particles are given as raw values (*_raw), and corrected for the dipped blank (*_dbcorr) using the median isotopic composition of the dipped blanks (Table 1). Measurement precision is 0.2 permil for samples greater than 20 mg C (1.7 mmol C) or 20 mg N (1.4 mmol N). \u00a0Only three samples did not meet this threshold for LPT C, but 77% of LPT samples did not meet this threshold for N. \u00a0d15N values for samples below the 20 mg N threshold were assigned a QF=4.<\/p>\n 2.2.3 Derived parameters: POM<\/p>\n Particulate organic matter (POM) is calculated from POC using a weight ratio of 1.88 g POM\/g POC (Lam et al., 2011).<\/p>\n POM [mg\/L] = POC [mM] * 12 [mg POC\/mmol POC] * 1.88 [g POM\/g POC]<\/p>\n 2.3 Particulate Inorganic Carbon (PIC) and CaCO3<\/p>\n 2.3.1 Analytical methodology for PIC<\/p>\n PIC was measured directly by coulometry (measurement of CO2 following closed-system conversion of PIC to CO2 upon addition of 1N phosphoric acid (WHOI) or 2 N sulfuric acid (UCSC) to a QMA punch or 1\/16 polyester prefilter) (Honjo et al., 1995)<\/p>\n 2.3.2 Data Processing<\/p>\n Intercomparability of the WHOI and UCSC datasets was assessed by checking that a PIC_LPT profile that had samples analyzed at both places was oceanographically consistent. \u00a0 Blank subtraction, error calculation, and detection limit determined as described at beginning of section 2.<\/p>\n 2.3.3 Derived parameters: CaCO3<\/p>\n The mass of CaCO3 is calculated stoichiometrically from the mass of PIC:<\/p>\n CaCO3 [ug\/L] = PIC [umol\/L] * 100.08 [mg CaCO3\/mmol PIC]<\/p>\n 2.4 Particulate trace metals (pTM)<\/p>\n 2.4.1 Analytical methodology for pTMs<\/p>\n Total pTM concentrations in the SPT were analyzed from either 1\/16 or 1\/8 subsamples of the top Supor (0.8um) filter. \u00a0pTM totals in the LPT were analyzed from 1\/8 subsamples (typically ~150L) of the QMA-side 51um pre-filter, which were rinsed at sea onto 25mm Supor (0.8um) filter discs using 0.2um-filtered surface seawater collected using clean techniques from an underway Fish system (Bruland et al., 2005). \u00a0Methods for particulate trace metal (pTM) digestion and analysis followed those described in (Ohnemus and Lam, 2015). \u00a0Briefly, the Supor filter matrix was first completely digested with the strongly oxidizing Piranha solution, a 3:1 mixture of sulfuric acid and hydrogen peroxide with 1 ppb Rh as a recovery monitor. \u00a0After drying down, remaining particles were digested in a mixture of HCl\/HNO3\/HF acids (all acids at 4 M) at 135\u02daC for 4 hrs, then dried down. Remaining pellet was reacted with 50% HNO3\/15% H2O2 to remove any remaining organics, dried, and resuspended in 5% HNO3\u00ad with 1 ppb In as an internal standard for analysis via high-resolution Inductively Coupled Mass Spectrometetry (HR-ICP-MS) using an Element-XR ICP-MS (Thermo Scientific) with SC-FAST (Elemental Scientific) sample introduction system and PC3 Peltier cooled quartz cyclonic spray chamber in the UCSC Plasma Analytical Facility. ICP-MS (Element XR, Thermo-Finnigan). \u00a0Elemental concentrations were standardized using multi-element, external standard curves prepared from NIST atomic absorption-standards in 5% HNO3\u00ad. \u00a0Instrument drift and matrix effects were corrected using the internal 1ppb In standard and monitored using a mixed element run standard. \u00a0Concentrations were determined using external standard curves of mixed trace elements standards.<\/p>\n A subset of samples were repeated in which samples were redigested and\/or rerun.<\/p>\n 2.4.2 Data processing<\/p>\n The reported error is calculated by propagating the two main sources of uncertainty: uncertainty in the dipped blank subtraction, and the combined uncertainty due to heterogeneity in particle distribution and variations in digestion. \u00a0The former is determined as the standard deviation of the dipped blank filters. The latter is determined from the maximum of the mean relative standard deviation of all repeat samples for that element if no repeats were run, and the actual standard deviation if repeats were run. \u00a0For most elements, the uncertainty in the blank subtraction was the largest source of uncertainty (Figure 1).<\/p>\n 2.4.3 Derived parameters<\/p>\n 2.4.3.1 Lithogenic material<\/p>\n Al is usually used as a tracer of lithogenic material since it is the third most abundant element in Earth\u2019s crust after Si and O. \u00a0Al has the added advantage that its concentration does not vary much between upper continental crust (UCC Al = 8.04% by weight) and bulk continental crust (BCC Al = 8.41wt%), so the estimate of lithogenic mass is not very sensitive to lithogenic source regions (Taylor and McLennan, 1995). \u00a0Slopes of Fe vs Al (10.3 mol Fe\/mol Al) and Fe vs Ti (0.23 mol Fe\/mol Ti) for samples dominated by lithogenic material (Station 1 trench) gave slopes that were very similar to UCC crustal ratios (Fe\/AlUCC=10.0 and Fe\/TiUCC-0.21; compare to Fe\/AlBCC=11.2 and Fe\/TiBCC-0.41). \u00a0We therefore use the UCC Al concentration of 8.04% to calculate lithogenic mass.<\/p>\n LITHO \u00a0[ug\/L] = Al [pM] * 27 [pg\/pmol] \/ 0.0804 [ug Al\/mg UCC] * [1ug\/1e6 pg]<\/p>\n 2.4.3.2 Fe and Mn oxyhydroxides<\/p>\n Fe and Mn in oxyhydroxides were calculated by subtracting Fe and Mn associated with lithogenic material. \u00a0Although Ti may be a better tracer for lithogenic Fe and Mn than Al as the ratios of Fe and Mn to Ti are less sensitive to source than ratios to Al (Lam et al., 2015), Ti concentrations were very low and often below the detection limit for this transect. \u00a0We verified that the UCC Fe:Al=0.210 mol\/mol and Mn:Al=0.00355 mol\/mol ratios (Taylor and McLennan, 1995) compared well with Fe:Al in the most lithogenic-rich sample in our dataset\u2014the deepest samples in the Peru Trench, which had Fe:Al=0.227. \u00a0We thus used the UCC ratios of Fe and Mn to Al to remove lithogenic Fe and Mn, We approximate the formulae for Fe and Mn oxyhydroxides to be Fe(OH)3 (ferrihydrite approximation) and MnO2 (birnessite approximation), with formula weights 106.9 g Fe(OH)3\/mol Fe and 86.9 g MnO2\/mol Mn, respectively. Negative numbers were set to 0.<\/p>\n FeOH3 [ug\/L] = (Fe [pM] \u2013 (Al [pM] * 0.210 [pmol Fe\/pmol Al]) ) * 106.9 [pg Fe(OH)3\/ pmol \u00a0Fe] * [1ug\/1e6 pg])<\/p>\n MnO2 [ug\/L] = (Mn [pM] \u2013 (Al [pM] * 0.00367 [pmol Mn\/pmol Al]) ) *<\/p>\n 86.9 [pg MnO2\/pmol Mn] * [1ug\/1e6 pg])<\/p>\n 2.5 Suspended particulate mass (SPM)<\/p>\n Suspended particulate mass in the sinking (>51um) and suspended (1-51um) size fractions was estimated as the chemical dry weight of the major particulate phases, which is the sum of POM, opal, CaCO3, lithogenic material, and Fe and Mn oxyhydroxides, and is calculated as:<\/p>\n SPM [mg\/L] = POM [ug\/L] + opal [ug\/L] + CaCO3 [ug\/L] + Litho [ug\/L] + Fe(OH)3 [ug\/L] + MnO2 [ug\/L]<\/p>\n Note that the resolution of this data is dictated by the lowest resolution of the component parts.<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/ocean-data.org\/schema\/hasBriefDescription":[{"@value":"Size-fractionated major and minor particle composition and concentration.","@language":"en-US"}],"http:\/\/purl.org\/dc\/terms\/description":[{"@value":" Size-fractionated major and minor particle composition and concentration.<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/www.w3.org\/2000\/01\/rdf-schema#label":[{"@value":"GP16 Particle Composition","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/hasProcessingDescription":[{"@value":" Blank corrections, error calculations, and detection limits are described above with analytical methodology.<\/p>\n Lab quality control (QC) included running standard reference materials for pTM (BCR414 and PACS-2\u2014see table 2), participation in intercalibration exercises, comparison of pTM profiles with Go-Flo pTM data (Sherrell\/Fitzsimmons\/Twining\/Ohnemus), and checks for oceanographic consistency.<\/p>\n All data have been assigned quality flags using the ODV convention and interpretation:<\/p>\n 0=good quality\u2014passed lab QC<\/p>\n 1=unknown quality\u2014oceanographically consistent, but no intercalibration possible<\/p>\n 4=questionable quality\u2014below detection limit or anomalously high or low<\/p>\n 8=bad quality \u2013failed lab QC, or known issue with sample<\/p>\n For barium (Ba_SPT_SPT_PUMP_CONC and Ba_LPT_PUMP_CONC), profiles shapes were sometimes strange in the middle water column (~500-2000m), diverging significantly from generally more oceanographically consistent Go-Flo pTM data. We suspect the occasional precipitation of an insoluble Ba mineral in our digestion procedure.\u00a0 For Ba, we\u2019ve threfore assigned QF=4 to all samples, and further assigned QF=8 to samples in which we most strongly suspect a problem.\u00a0<\/p>\n <\/p>\n <\/p>\n \u00a0<\/p>\n BCO-DMO Data Processing Notes:<\/strong><\/p>\n -Reformatted column names to comply with GEOTRACES and BCO-DMO standards.
\n-Replaced all blanks, \"NaN\", \"#N\/A\", and \"#VALUE!\" with \"nd\".
\n-version 12\/06\/2016: original submission
\n-version 01\/03\/2017: error in small size fraction (SPT) particulate trace metals for station 3. Correction affects most SPT parameters for station 3.
\n-version 01\/24\/2017: corrected ISO_DateTime_UTC for events 4013 and 4150.<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/purl.org\/dc\/terms\/identifier":[{"@value":"668083","@type":"xsd:int"}],"http:\/\/purl.org\/dc\/terms\/title":[{"@value":"GP16 Particle Composition"}],"http:\/\/purl.org\/dc\/terms\/date":[{"@value":"2016-12-02T13:02:48-05:00","@type":"xsd:dateTime"}],"http:\/\/purl.org\/dc\/terms\/created":[{"@value":"2016-12-02T13:02:48-05:00","@type":"xsd:dateTime"}],"http:\/\/purl.org\/dc\/terms\/modified":[{"@value":"2023-07-07T16:10:26-04:00","@type":"xsd:dateTime"}],"http:\/\/rdfs.org\/ns\/void#inDataset":[{"@id":"http:\/\/www.bco-dmo.org\/"}],"http:\/\/ocean-data.org\/schema\/namedGraph":[{"@value":"urn:bcodmo:dataset:668083","@type":"xsd:token"}],"http:\/\/ocean-data.org\/schema\/osprey_page":[{"@id":"https:\/\/www.bco-dmo.org\/dataset\/668083"}],"http:\/\/ocean-data.org\/schema\/identifier":[{"@value":"_:Identifier668083"}],"http:\/\/ocean-data.org\/schema\/datasetTitle":[{"@value":"Size-fractionated major and minor particle composition and concentration collected from RV Thompson (TN303) along the US GEOTRACES EPZT transect in the Eastern Tropical Pacific during 2013 (US GEOTRACES EPZT project)","@language":"en-US"}],"http:\/\/ocean-data.org\/schema\/abstract":[{"@value":"Size-fractionated major and minor particle composition and concentration collected from RV Thompson (TN303) along the US GEOTRACES EPZT transect in the Eastern Tropical Pacific during 2013 (US GEOTRACES EPZT project)","@language":"en-US"}],"http:\/\/purl.org\/dc\/terms\/rights":[{"@id":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"http:\/\/ocean-data.org\/schema\/deprecated":[{"@value":"false","@type":"xsd:boolean"}],"http:\/\/ocean-data.org\/schema\/temporalExtent":[{"@value":"_:temporalExtent668083"}],"http:\/\/purl.org\/dc\/terms\/bibliographicCitation":[{"@value":"Lam, P. (2017) Size-fractionated major and minor particle composition and concentration collected from RV Thompson (TN303) along the US GEOTRACES EPZT transect in the Eastern Tropical Pacific during 2013 (US GEOTRACES EPZT project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). 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