In these experiments, dissolved Fe(II) concentrations were measured over time for the neutrophilic iron-oxidizing bacterium (FeOB) Mariprofundus ferrooxydans PV-1 as well as the FeOB "TAG-1" (An isolate from hydrothermal vents in the Mid Atlantic ridge that has not yet been formally named. TAG simply refers to the TAG hydrothermal site from which this microbe was isolated.). These experiments measure how Fe(II) concentration changes over time in culture media at 100 µM oxygen at 20 degrees C by sampling daily for 5 days.

Methods & Sampling

These data were collected by placing each strain in a 100 mL serum vial with 6 mL of their standard, published media with 30 mg zero valent iron as a source of Fe(II). The headspace was filled with a gas mix of 8% oxygen/10% carbon dioxide/82% nitrogen by using bottled gas mixes and a regulator to flush the headspace without overpressurization. Each vial was sampled once per day and the Fe(II) concentration was determined by ferrozine assay.

Data Processing Description

BCO-DMO Data Processing Notes:
-Reformatted column names to comply with BCO-DMO standards
-Reformatted dates from mm/dd/yy to yyyy/mm/dd
-Replaced spaces with underscores

Data Files

File
timecourses.csv (Comma Separated Values (.csv), 2.06 KB) MD5:4d3ec3ed77b152ec87fa639fbe098580 Primary data file for dataset ID 709440

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1459252

Deployments

None available yet

Instruments

Spectrophotometer

Supplied Name: Cary 100 UV-Vis Spectrophotometer (Agilent Technologies)

Supplied Description:

Used to measure ferrozine assay

Instrument Type

Generic Name: Spectrophotometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/

Generic Description:

An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
vial	[strain] ASWkan replacement description	unitless	sample
date	Date measurement was taken; YYYY/MM/DD	unitless	date
time	Time since start of experiment	hours	time
Fe_II_concentration	Concentration of Fe(II) in media (average of triplicate samples)	mM	Fe
standard_deviation	Standard deviation of triplicate samples	mM	std_dev