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Measurements of photosynthesis and respiration of Ulva lactuca occurred at the end of 3 weeks of acclimation to various levels of ocean acidification at 15\u00b0C. Small circular sections of U. lactuca thalli used for measurements were sampled with a cork borer the evening before measurement and returned to culture conditions overnight to allow for wound healing. Plant sections used to estimate rates of photosynthesis and respiration from oxygen exchange measurements were placed in small white, mesh threaded bags designed to fit the diameter of the cuvette chamber so that the plant material was oriented perpendicularly to the light source provided in the Qubit Systems Dissolved Oxygen package. Prior to introducing plants to the cuvette chamber, oxygen exchange between the stirred seawater and electrode system (i.e., oxygen consumption by the electrode) was monitored to adjust for its effect on subsequent measurements in the presence of plant material. Sections were transferred to the cuvette containing a magnetic stirring bar and 3 ml of seawater corresponding to the average pCO2 level in their particular culture pot and placed in darkness for 10-15 minutes prior to beginning measurement. Once measurements began, output of oxygen exchange was monitored until a steady state of oxygen exchange was attained (typically within 10 minutes) in either darkness or light. Photosynthesis and respiration were measured at 15\u00b0C and ~550, and 0, \u00b5mole photons m-2 s-1, respectively; the same temperature and light conditions as in growth culture. Respiration was measured both following a \u226510 minute acclimation period in darkness prior to light exposure (dark respiration) and again in darkness immediately following exposure to the saturating light intensity (as an estimate of respiration that occurs in the light). Following these measurements in seawater at their culture pCO2 level, the seawater in the cuvette was replaced with standard air-saturated seawater at 15\u00b0C and the measurement process described above was repeated for measurements of respiration in the dark and \u2018light\u2019 and photosynthesis. After measurements of gas exchange, the mass of plant tissue was measured on an analytical balance.<\/p>\n
Note: Trial 1 was a pilot test of culture system and methodological procedures so was not used for data collection.<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/ocean-data.org\/schema\/hasBriefDescription":[{"@value":"Ulva photosynthesis and respiration","@language":"en-US"}],"http:\/\/purl.org\/dc\/terms\/description":[{"@value":"
This dataset includes rates of photosynthesis and respiration by Ulva during culture experiments grown at 15 degrees C under various pCO2 levels, during May through July 2015.<\/p>\n
Related Datasets:<\/strong> BCO-DMO Processing Notes:<\/strong>
Ulva: Carbonate chemistry pCO2<\/a>: Carbonate chemistry of Ulva lactuca culture pots testing the effects of pCO2 variability (Seaweed OA Resilience project)
Ulva: Chl a<\/a>: Chlorophyll a per unit biomass in Ulva lactuca under ocean acidification (OA) conditions (Seaweed OA Resilience project)
Ulva: CHN and stable isotopes<\/a>: Stable isotope ratios and mass of carbon and nitrogen in Ulva cells under ocean acidification conditions (Seaweed OA Resilience project)
Ulva: Growth<\/a>: Growth rates of Ulva exposed to different average and variability of pCO2 (Seaweed OA Resilience project)
Ulva: pH and temperature time-series<\/a>: Time-series at 10 minute sampling interval of pH and temperature in Ulva culture pots (Seaweed OA Resilience project)
Ulva: pH Drift<\/a>: Carbonate chemistry over a time course with Ulva in pH drift experiments (Seaweed OA Resilience project)
Ulva: seawater delta13C<\/a>: Stable isotope ratio and concentration of carbon in seawater from Ulva OA experiments (Seaweed OA Resilience project)<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/www.w3.org\/2000\/01\/rdf-schema#label":[{"@value":"Ulva: Photosynthesis and respiration","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/hasProcessingDescription":[{"@value":"
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