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Water samples for chlorophyll extraction were collected from the mesocosms via a 5 L Niskin. Chlorophyll samples were filtered in triplicate through a 25mm Glass Fiber Filter (GFF), and immediately extracted in 6 mL of ethanol for 12-18 hours in the dark at room temperature. After extraction, filters were removed from the sample, and the fluorescence of the sample was read on a Turner AU10. The sample was then acidified with 1 drop of 10% HCL and re-read on the same instrument. The fluorometer was calibrated prior to using with a chlorophyll standard purchased from Sigma.<\/p>\n
Mesocosm treatments are as follows:
\nP-limited: N:P added in a 60:1 ratio during the first 3 days of the experiment, no shading
\nRedfield: N:P added in a 16:1 ratio during the first 3 days of the experiment, no shading
\nShaded: N:P added in a 16:1 ratio during the first 3 days of the experiment, top shaded of the mesocosm added on May 20, 2017
\nAmbient: no nutrients added, no shading
\nSamples were taken from either 1m, 5m, or from sediment from settling cones. Samples were noted in the Sample Depth (m) column.<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/www.w3.org\/2000\/01\/rdf-schema#label":[{"@value":"MesoHux Chlorophyll","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/hasProcessingDescription":[{"@value":"
BCO-DMO Processing Notes:<\/p>\n