Dataset: Ocean Crust Microbiome Amplicon Metadata
Data Citation:
Orcutt, B. N., D'Angelo, T. (2020) Synthesis of publicly-available sequence datasets of the 16S rRNA gene in environmental DNA extracted from seafloor and subseafloor samples from the Dorado outcrop, Lō'ihi Seamount, North Pond, and Juan de Fuca Ridge flank. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-02-04 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.789136.1 [access date]
Terms of Use
This dataset is licensed under Creative Commons Attribution 4.0.
If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.
DOI:10.1575/1912/bco-dmo.789136.1
Spatial Extent: N:45.62 E:-46 S:10 W:-155.27
Temporal Extent: 2014 - 2019
Project:
Microbial activity in the crustal deep biosphere
(Slow Life in Crust)
Principal Investigator:
Beth N. Orcutt (Bigelow Laboratory for Ocean Sciences)
Co-Principal Investigator:
Timothy D'Angelo (Bigelow Laboratory for Ocean Sciences)
BCO-DMO Data Manager:
Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)
Version:
1
Version Date:
2020-02-04
Restricted:
No
Validated:
Yes
Current State:
Final no updates expected
Synthesis of publicly-available sequence datasets of the 16S rRNA gene in environmental DNA extracted from seafloor and subseafloor samples from the Dorado outcrop, Lō'ihi Seamount, North Pond, and Juan de Fuca Ridge flank
Abstract:
To summarize crustal bacterial and archaeal taxa for this review, we synthesized publicly-available sequence datasets of the 16S rRNA gene in environmental DNA extracted from seafloor and subseafloor basalts generated using 454, Illumina and Ion Torrent amplicon platforms. These include seafloor basalts from the Dorado Outcrop and the Lō'ihi Seamount in the Pacific Ocean and subseafloor basalts from North Pond on the western flank of the Mid-Atlantic Ridge and the Juan de Fuca Ridge flank in the northeastern Pacific Ocean. Datasets from rock colonization experiments conducted in the subseafloor at the Juan de Fuca Ridge flank site were also included, as well as microbial community surveys of the subseafloor crustal fluids from the anoxic Juan de Fuca site and the oxic North Pond site.