Dataset: Continuous culture studies of Synechococcus elongatus CCMP1629 in nitrate-limited and nutrient-replete cultures plus DNA results

This dataset has not been validatedUnder revisionDOI: 10.26008/1912/bco-dmo.811093.1Version 2 (2023-04-28)Dataset Type:experimental

Principal Investigator: Edward Laws (Louisiana State University College of the Coast and Environment)

Co-Principal Investigator: Uta Passow (University of California-Santa Barbara)

Scientist: Stuart Alexander McClellan (Louisiana State University College of the Coast and Environment)

BCO-DMO Data Manager: Dana Stuart Gerlach (Woods Hole Oceanographic Institution)

Project: Collaborative Research: Effects of multiple stressors on Marine Phytoplankton (Stressors on Marine Phytoplankton)

Abstract

This dataset summarizes the results of continuous culture studies of the marine cyanobacterium Synechococcus CCMP 1629 grown under controlled conditions at temperatures from 20 to 45 degrees Celsius, nutrient-replete and nitrate-limited conditions, high and low CO2 partial pressures (1000 and 400 ppm CO2), and high and low irradiance (300 and 50 micro mol quanta per square meter per second). The dataset covers a total of 48 steady states (6 temperatures x 2 CO2 partial pressures x 2 irradiances ...

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Data File(s)TypeDescriptionAction

Supplemental File(s)TypeDescriptionAction
Fig1_chemostat_diagram.pdf (239.11 KB)
Portable Document Format (.pdf)Diagram of chemostat with three-way valve for bubbling air and collecting samples.
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FigS2_photosynthetic_rate.pdf (340.56 KB)
Portable Document Format (.pdf)Figure S2. Estimated photosynthetic rate from 5-minute uptake of 14C at 10C and 50 mol photons m–2 s–1. The smooth curve is a hyperbolic tangent fit to the data by least squares.
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Table1_seawater_characteristics.pdf (243.05 KB)
Portable Document Format (.pdf)Table 1. Characteristics of seawater with a total alkalinity of 2365 meq L–1 as a function of pCO2 and temperature based on equations in Zeebe and Wolf-Gladrow, CO2 in Seawater: Equilibrium, Kinetics, Isotopes (2001)
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Supplemental Files
Filename: Fig1_chemostat_diagram.pdf (239.11 KB)
Type: Portable Document Format (.pdf)
Description: Diagram of chemostat with three-way valve for bubbling air and collecting samples.
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Filename: FigS2_photosynthetic_rate.pdf (340.56 KB)
Type: Portable Document Format (.pdf)
Description: Figure S2. Estimated photosynthetic rate from 5-minute uptake of 14C at 10C and 50 mol photons m–2 s–1. The smooth curve is a hyperbolic tangent fit to the data by least squares.
Download
Filename: Table1_seawater_characteristics.pdf (243.05 KB)
Type: Portable Document Format (.pdf)
Description: Table 1. Characteristics of seawater with a total alkalinity of 2365 meq L–1 as a function of pCO2 and temperature based on equations in Zeebe and Wolf-Gladrow, CO2 in Seawater: Equilibrium, Kinetics, Isotopes (2001)
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The marine cyanobacterium Synechococcus elongatus clone CCMP1629 was grown in a continuous culture system on a 14:10 light-dark cycle under either nitrate-limited or nutrient-replete conditions, a photoperiod irradiance of either 50 or 300 micro-mol photons per square meter per second, partial pressures of either 400 or 1000 ppm CO2, and temperatures ranging from 20 to 45 degrees Celsius. Growth rates, photosynthetic rates, respiration rates, C:N ratios, C:Chlorophyll-a ratios, productivity indices, Fv/Fm ratios, and the initial slope and light-saturated asymptote of short-term photosynthesis-irradiance curves are reported.

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Methods
Dickson, A.G., Sabine, C.L. and Christian, J.R. (Eds.) 2007. Guide to best practices for ocean CO2 measurements. PICES Special Publication 3, 191 pp. ISBN: 1-897176-07-4. URL: https://www.nodc.noaa.gov/ocads/oceans/Handbook_2007.html

Laws, E., Passow, U. (2020) Continuous culture studies of possible climate change effects: Synechococcus elongatus CCMP1629 growth in nitrate-limited and nutrient-replete cultures. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-05-11 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.811093.1 [access date]

Terms of Use

This dataset is licensed under Creative Commons Attribution 4.0.


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Funded by the U.S. National Science Foundation