Dataset: Red Sea Gradient Fv/Fm

Name: Coral physiology data (Fv/Fm) on four collected coral species from six sites in the Red Sea from 2018 to 2020
Published: 2021-11-03
Keywords: ocean warming, Red Sea, thermal stress, coral thermotolerance, standardized acute assays, Coral Bleaching Automated Stress System (CBASS), oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.863771.1

Spatial Extent: N:29.5018 E:43.3131 S:11.9586 W:34.9179

Temporal Extent: 2018-07-30 - 2020-03-02

Project:

EAGER: Collaborative Research: Bleaching phenotypes of acute vs. chronic coral bleaching susceptibility and resilience: towards a standardized coral resilience diagnostic (EAGER-CBASS)

Principal Investigator:

Daniel J. Barshis (Old Dominion University, ODU)

Co-Principal Investigator:

Christian R. Voolstra (University of Konstanz, UK)

Scientist:

Nicolas R. Evensen (Old Dominion University, ODU)

Contact:

Nicolas R. Evensen (Old Dominion University, ODU)

BCO-DMO Data Manager:

Taylor Heyl (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2021-11-03

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Coral physiology data (Fv/Fm) on four collected coral species from six sites in the Red Sea from 2018 to 2020

Abstract:

This dataset contains measurements of coral physiology (Fv/Fm) during 18-hour acute thermal stress experiments, where temperatures were ramped up to 30, 33, 36, and 39 degrees Celsius. Four coral species (Acropora hemprichii, Pocillopora verrucosa, Porites lobata, and Stylophora pistillata) were sampled from six sites along the length of the Red Sea, down to Djibouti in the Gulf of Aden.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2021-11-03	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.863771.1	2021-11-08

Methods & Sampling

Data are from experiments performed across multiple sites in Israel, Saudi Arabia, and Djibouti. Multiple ramets from seven genets of Acropora hemprichii, Pocillopora verrucosa, Porites lobata, and Stylophora pistillata were collected from six sites along the Red Sea and used in an 18-hour acute thermal stress assay using the Coral Bleaching Automated Stress System (CBASS).

Corals were subjected to 18-hour acute thermal profiles with four peak target temperatures (30°C, 33°C, 36°C, and 39°C). Experimental tanks were ramped up from the 30 degrees Celsius control treatment to temperature treatments reaching 33°C, 36°C, and 36.°C in the prolonged experiment at rates of 0.5°C and 1.5°C per day. Each temperature treatment contained two replicate tanks (A and B).

Physiological responses (Fv/Fm) of the coral host and associated endosymbionts were measured during the experiments. "Fv/Fm" refers to measurements of dark-adapted maximum quantum yield of photosystem II of the algal symbionts (Fv/Fm) for each ramet. Fv/Fm was measured using a Pulse amplitude-modulated (PAM) fluorometer (Diving PAM or Imaging PAM, WALZ).

Problems/Issues:
There are some missing data due to sample loss or mortality.

Data Processing Description

Data Processing:
Data were organized using Microsoft Excel and R statistical software (version 4.0.3).

BCO-DMO Processing:
- Adjusted field/parameter names to comply with BCO-DMO naming conventions;
- Added a conventional header with dataset name, PI names, version date;
- Converted Collection_Date to YYYY-MM-DD format.

Originally submitted GitHub repository https://github.com/BarshisLab/Gradient-physiology was forked to https://github.com/BCODMO/Gradient-physiology/tree/v1.0 for curation purposes and tagged with release 1.0, which corresponds with this dataset submission. The code is attached to this record as a Supplemental File. Note the original repository may have continued updates.

Data Files

File
Red_Sea_FvFm.csv (Comma Separated Values (.csv), 71.01 KB) MD5:0b97e512e1c76e09d7972ca2f2f73942 Primary data file for dataset ID 863771

Supplemental Files

File
Gradient-physiology-1.0.zip (ZIP Archive (ZIP), 24.45 MB) MD5:23bd53d1798c0cce2c4822f0c7543bcb Code used to analyze and plot data; associated with BCO-DMO datasets 863771, 863786, 863800. These files are also available in the following GitHub repository: https://github.com/BCODMO/Gradient-physiology/releases/tag/v1.0

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1833201

Deployments

None available yet

Instruments

Coral Bleaching Automated Stress System

Supplied Name: CBASS

Supplied Description:

Instrument Type

Generic Name: Coral Bleaching Automated Stress System

Acronym: CBASS

Generic Description:

CBASS, which stands for "Coral Bleaching Automated Stress System", are portable, field-deployable experimental tanks used to apply rapid, acute heat stress challenges. This system is described in:

Voolstra, C. R., Buitrago‐López, C., Perna, G., Cárdenas, A., Hume, B. C. C., Rädecker, N., & Barshis, D. J. (2020). Standardized short‐term acute heat stress assays resolve historical differences in coral thermotolerance across microhabitat reef sites. Global Change Biology, 26(8), 4328-4343. Portico. https://doi.org/10.1111/gcb.15148

Fluorometer

Supplied Name: Pulse amplitude-modulated (PAM) fluorometer (Diving PAM or Imaging PAM, WALZ)

Supplied Description:

Instrument Type

Generic Name: Fluorometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/113/

Generic Description:

A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Collection_Date	date of collection; format: YYYY-MM-DD Format: %Y-%m-%d	unitless	date
Site_Latitude	latitude North	decimal degrees	lat
Site_Longitude	longitude East (West is negative)	decimal degrees	lon
Sample	sample identifier	unitless	sample
Site	sampling site	unitless	site
Species	species name	unitless	species
Genotype	genotype identifier	unitless	no_bcodmo_term
Temperature	temperature in tank	degrees Celsius	temp
Replicate	replicate identifier (A or B)	unitless	replicate
FvFm	measurement of dark-adapted maximum quantum yield of photosystem II of the algal symbionts (Fv/Fm) for each ramet	unitless	Fv_Fm

Status message

Database

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Dataset: Red Sea Gradient Fv/Fm