Dataset: Mesocosm study: seawater chemistry

Name: Water chemistry during mesocosm study of trophic interactions under ocean acidification in Bodega Bay, CA.
Published: 2022-03-09
Keywords: carbon dioxide, Tidepool, Predator-prey interaction, benthic invertebrates, behavior, non-consumptive effects, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.869110.1

Spatial Extent: N:38.33325 E:-123.04805 S:38.33325 W:-123.04805

Temporal Extent: 2015-07-11 - 2015-07-21

Project:

Trophic consequences of ocean acidification: Intertidal sea star predators and their grazer prey (BOAR Trophic)

Principal Investigator:

Brian Gaylord (University of California-Davis, UC Davis-BML)

Student:

Brittany Jellison (University of New Hampshire, UNH)

Contact:

Brittany Jellison (University of New Hampshire, UNH)

BCO-DMO Data Manager:

Taylor Heyl (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2022-03-09

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Water chemistry during mesocosm study of trophic interactions under ocean acidification in Bodega Bay, CA.

Abstract:

This dataset provides water chemistry data during a mesocosm study of trophic interactions among intertidal sea stars (Leptasterias hexactis), snails (Tegula funebralis), and macroalgae (Mazzaella flaccida) under ocean acidification in Bodega Bay, CA.

Expand/Collapse All

Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2022-03-09	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.869110.1	2022-03-16

Methods & Sampling

This dataset is part of a larger experiment to investigate how pH influences trophic links between intertidal sea stars (Leptasterias hexactis), snails (Tegula funebralis), and macroalgae (Mazzaella flaccida). Organisms were placed for 7 days in mesocosms containing seawater at either ambient (~7.9) or low pH (~7.0). The pH was modified using equimolar additions of sodium bicarbonate (NaHCO3) and hydrochloric acid (HCl). The water in each container was changed daily. The mesocosm array consisted of 40, 13-liter (L) circular plastic containers with a mesh barrier down the center to separate predator, prey, and/or basal resource but allowing for passage of waterborne cue. Mesocosms were filled halfway with seawater, allowing 10 centimeter (cm) of refuge space for snails above the waterline. Mesocosms were held within a seawater table under constant flow to maintain consistent temperatures.

Temperature, salinity, dissolved oxygen, and pH were measured using a YSI ProPlus Sensor.

Total pH was measured using a Sunburst SAMI spectrophotometric unit modified for benchtop use.

Total alkalinity was measured via Gran titration (Riebesell et al. 2010) standardized using certified reference material (A. Dickson, Scripps Institution of Oceanography) using a Metrohm 855 autotitrator.

Values of total pH for each container were measured using a YSI ProPlus Sensor and calibrated to the total scale using daily samples run on a Sunburst SAMI spectrophotometric unit modified for benchtop use.

Data Processing Description

pCO2 was calculated from daily alkalinity and pH using the seacarb package in R assuming the default dissociation constants.

BCO-DMO processing description:
- Adjusted field/parameter names to comply with BCO-DMO naming conventions
- Replaced blank values with "nd" (no data)
- Added a conventional header with dataset name, PI names, version date

Data Files

File
mesocosm_study_-_seawater_chemistry.csv (Comma Separated Values (.csv), 86.86 KB) MD5:6514617f46d4d8c9314bf6ac61840470 Primary data file for dataset ID 869110

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1636191

Deployments

None available yet

Instruments

Automatic titrator

Supplied Name: Metrohm 855 autotitrator

Supplied Description:

Instrument Type

Generic Name: Automatic titrator

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/

Generic Description:

Instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached.

Benchtop pH Meter

Supplied Name: Sunburst SAMI spectrophotometric unit

Supplied Description:

Instrument Type

Generic Name: Benchtop pH Meter

Generic Description:

An instrument consisting of an electronic voltmeter and pH-responsive electrode that gives a direct conversion of voltage differences to differences of pH at the measurement temperature. (McGraw-Hill Dictionary of Scientific and Technical Terms)

This instrument does not map to the NERC instrument vocabulary term for 'pH Sensor' which measures values in the water column. Benchtop models are typically employed for stationary lab applications.

YSI Professional Plus Multi-Parameter Probe

Supplied Name: YSI ProPlus Sensor

Supplied Description:

Instrument Type

Generic Name: YSI Professional Plus Multi-Parameter Probe

Acronym: YSI ProPlus

Generic Description:

The YSI Professional Plus handheld multiparameter meter provides for the measurement of a variety of combinations for dissolved oxygen, conductivity, specific conductance, salinity, resistivity, total dissolved solids (TDS), pH, ORP, pH/ORP combination, ammonium (ammonia), nitrate, chloride and temperature. More information from the manufacturer.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Container	Container number	unitless	number
Phase	pH treatment level of the container; Low pH ~ 7.0 total pH, Ambient pH ~7.9 total pH	unitless	pH
Trophic_Treatment	Trophic treatment condition; “no-predator” = four snails and four circular pieces of macroalgae on one side of the barrier, “cue only" = one sea star was housed on one side of the barrier with four snails and macroalgae on the other side, "complete interaction" = one sea star, four snails, and macroalgae all placed on one side of the barrier together, “no prey/no grazing” = one sea star was placed on one side of the barrier with the macroalgae on the other.	unitless	treatment
pH	pH Exposure = 5 day exposure phase before the mesocosm component in which animals were held separately and exposed to ambient and low pH, mesocosm = 7 day mesocosm component of the experimnet in which sea stars, snails and macroalgae were held together under 4 trophic treatments and exposed to the same pH level as they were during the pH exposure phase of the experiment.	unitless	pH
Day	Day number within the experiment	day number	day
Water_Change	Whether water quality measurements were taken before or after daily complete water changes	unitless	no_bcodmo_term
Conductivity	Conductivity measured by a YSI ProPlus sensor	mS/cm	cond
Salinity	Salinity measured by a YSI ProPlus sensor	ppt	sal_ss
Dissolved_Oxygen	Dissolved Oxygen measured by a YSI ProPlus sensor	mg/L	O2
pH_mV	pH millivolts measured by a YSI ProPlus sensor	mV	pH
Temperature	Temperature measured by a YSI ProPlus sensor	degrees Celsius	temp
Total_pH	Total pH values for each container were processed using measurements from a YSI ProPlus Sensor and calibrated to the total scale using daily samples run on a Sunburst SAMI	pH units	pH
Alkalinity	Total alkalinity was measured via Gran titration (Riebesell et al.2010) standardized using certified reference material (A. Dickson, Scripps Institution of Oceanography) using a Metrohm 855 autotitrator	µmol/kgSW	TALK
pCO2	pCO2 was calculated from daily alkalinity and pH using the seacarb package in R assuming the default dissociation constants.	ppm	pCO2
SAMI_total_pH	Total pH was measured using a Sunburst SAMI spectrophotometric unit modified for benchtop use	pH units	pH

Database

Contribute Data

Dataset: Mesocosm study: seawater chemistry