Dataset: Nitrifier CN

Name: Cellular carbon (C) and nitrogen (N) measurements of marine nitrifiers
Published: 2022-03-11
Keywords: oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.871075.1

Spatial Extent: N:34.413962 E:-119.84912 S:34.413962 W:-119.84912

Temporal Extent: 2018-03-12 - 2021-08-20

Project:

Collaborative Research: Underexplored Connections between Nitrogen and Trace Metal Cycling in Oxygen Minimum Zones Mediated by Metalloenzyme Inventories (CliOMZ)

Principal Investigator:

Alyson E. Santoro (University of California-Santa Barbara, UCSB)

Contact:

Barbara Bayer

BCO-DMO Data Manager:

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2022-03-11

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Cellular carbon (C) and nitrogen (N) measurements of marine nitrifiers

Abstract:

This dataset contains cellular carbon (C) and nitrogen (N) measurements of marine nitrifiers. These data were generated through experiments conducted at the University of California, Santa Barbara over a period of time from March 2018 to August 2021.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2022-03-11	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.871075.1	2022-03-17

Methods & Sampling

100-500 mL of culture was filtered onto combusted (450°C, 4h) glass fiber filters (Advantec, GF-75, 25mm). Filters were acidified with HCl (10% v/v), dried (60°C, 24 h), and packed into tin capsules prior to being analyzed on a CHN elemental analyzer (Exeter Analytical, CEC 440HA). The instrument was calibrated with acetanilide following manufacturer protocols.

A detailed description of materials and methods can be found in Bayer et al. 2022.

Data Processing Description

Data Processing:
Cellular carbon (C) content was calculated by dividing total particulate organic C by the number of newly produced cells.

BCO-DMO Processing:
- renamed fields to conform with BCO-DMO naming conventions;
- replaced "NA" with "nd" (no data).

Data Files

File
nitrifier_cn.csv (Comma Separated Values (.csv), 1.29 KB) MD5:b3e362853af0a2c0ee86293fec8108b9 Primary data file for dataset ID 871075

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1924512

Deployments

None available yet

Instruments

CHN Elemental Analyzer

Supplied Name: CHN elemental analyzer (Exeter Analytical, CEC 440HA)

Supplied Description:

Instrument Type

Generic Name: CHN Elemental Analyzer

Acronym: CHN_EA

Generic Description:

A CHN Elemental Analyzer is used for the determination of carbon, hydrogen, and nitrogen content in organic and other types of materials, including solids, liquids, volatile, and viscous samples.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Nitrifier_strain	Name of cultured nitrifier	unitless	taxon
Growth_phase	Growth phase of the culture when the measurement was taken (EEXP, early exponential growth; LEXP, late exponential growth; STAT, stationary phase)	unitless	growth
Filtered_volume	Culture volume filtered onto glass fiber filter	liters (L)	volume
Blank_corrected_C	Amount of organic carbon corrected for blank (culture medium filtered onto glass fiber filter)	microgram carbon (ug C)	C
Blank_corrected_N	Amount of nitrogen corrected for blank (culture medium filtered onto glass fiber filter)	microgram nitrogen (ug N)	N
C_to_N_molar	Molar C:N ratio of biomass	moles of carbon per mole of nitrogen (mol C/mol N)	mole_ratio
Cell_abundance	Cell numbers of cultured nitrifiers	cells per liter (cells/L)	abundance
C_content	Cellular carbon content	femtogram per cell (fg/cell)	C

Database

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Dataset: Nitrifier CN