Dataset: Multiplexed Microfluidic Screening of Bacterial Chemotaxis Modeling Results from 2019-2022 (VIC project)

Primary data file for dataset ID 885701, version 1

Maximum accumulation responses for each tested organism (V. alginolyticus, P. haloplanktis, E. coli) across a range of concentrations and chemicals. Corresponds to Fig. 4 in Stehnach et al. (Multiplexed microfluidic screening of bacterial chemotaxis).

MATLAB codes used to process and characterise the performance of the MCD channels. Includes a brief summary file.

Evolution of the cell positions of V. alginolyticus in a microfluidic channel with an evolving chemical gradient of a model chemoattractant (serine). Corresponds to Fig2d in Stehnach et al (Multiplexed microfluidic screening of bacterial chemotaxis)

Accumulation plots for chemotaxis experiments (MCD) for the following organism/chemical pairings: V. alginolyticus (VA) and phenol VA and leucine P. haloplanktis (PH) and serine E. coli (EC) and serine). Corresponds to Figs 3bdfh in Stehnach et al (Multiplexed microfluidic screening of bacterial chemotaxis

Channel masks to reproduce the three wafer molds in order to fabricate the MCD (multiplexed chemotaxis device).

Measured chemical concentrations generated from the dilution microchannels for various driving pressures in each observation region

The uniformity of the chemostimulus gradient evolution across the various observation regions (C0-C5) was determined through fluorescent dye (fluorescein) visualization of stop-flow experiments with pressure 100 mbar (dilution layer) and 70 mbar (cell injection layer)

Conditional probability plots for chemotaxis experiments (MCD) for the following organism/chemical pairings: V. alginolyticus (VA) and phenol VA and leucine P. haloplanktis (PH) and serine E. coli (EC) and serine). C0 = 200uM, C1 = 49uM, C2 = 3.6uM, C3 = 0.12uM, C4 = 0.012uM, C5 = 0uM (Control) Corresponds to Figs 3aceg in Stehnach et al (Multiplexed microfluidic screening of bacterial chemotaxis

Mixing performance: The degree of mixing (DOM) was quantified for a long test channel having 29 herringbone cycles for two different flow rates: 22 uL\min and 222 uL\min using fluorescein dye

Control chemotaxis experiments with V. alginolyticus to test reproducability between observation channels. Tested: no gradient (ASW for all inputs) and a fixed gradient of 200uM (serine) for all channels. Includes kymographs and accumulation curves, Fig. 9 and Fig. 2kl respectively of Stehnach et al (Multiplexed microfluidics of bacterial screening)

Chemostimulus distribution: The normalized measured widths of the chemical, cell, and buffer streams illustrate their high degree of symmetry across all observation channels

Comparison of the chemotactic responses of V. alginolyticus between the single assays (SA) and the MCD for the model chemoattract serine at 6 concentrations: 200, 49, 3.6, 0.12, 0.012,0uM. Includes: Accumulation index plots for both conditions (Fig f,h) Sorenson similarity measurement between the conditional probability plots of the two devices (Fig 2i) Comparison of the peak chemotactic response...

Conditional probability distributions of the chemotactic responses of V. alginolyticus in MCD for the model chemoattract serine at 6 concentrations: 200, 49, 3.6, 0.12, 0.012,0uM. Includes: Corresponds to Fig 2g of Stehnach et al (Multiplexed microfluidic screening of bacterial chemotaxis)

Conditional probability distributions of the chemotactic responses of V. alginolyticus in single assays (SA) for the model chemoattract serine at 6 concentrations: 200, 49, 3.6, 0.12, 0.012,0uM. Corresponds to Fig 2e of Stehnach et al (Multiplexed microfluidic screening of bacterial chemotaxis)